Creation of the Transposon Mutant Library: Transfer of the Transposon into Leptospira spp. by Conjugation
3:56
Animal Experiment
4:57
Making the Genomic Library
9:24
Results: Challenged Hamsters
10:59
Conclusion
Transcription
The overall goal of this high-throughput transposon sequencing technique is to identify and quantify transposon mutants with in vivo and in vitro fitness defects, and thereby, identify Leptospira genes involved in virulence. This method can help a
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We describe here a technique that combines transposon mutagenesis with high-throughput sequencing to identify and quantify transposon leptospiral mutants in tissues after a challenge of hamsters. This protocol can be used to screen mutants for survival and dissemination in animals and can also be applied to in vitro studies.