Coating of Microtiter Plates with α4β1 Integrin for Ex Utero Allantois Explant Culture
1:54
Uterus Dissection from a Pregnent Mouse at DPC 8
3:24
Embryo and Allantois Dissection and Ex Utero Allantois Cultre
6:18
Staining Explanted Allantoides with Endothelial Cell Marker CD31
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Results: Allantois Attachment and Immunohistochemistry
8:54
Conclusion
Transcription
The overall goal of this procedure is the dissection and explant culture of murine allantois for the analysis of allantoic attachment in vitro. This method can help answer key questions in the field of mammalian embryo development, such as the con
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We describe an in vitro assay to model chorioallantoic attachment, the first step in placenta formation. The protocol demonstrates the dissection and explant culture of murine allantoides on immobilized α4β1 integrin. Allantois attachment is evaluated microscopically at pre-determined time points.