Preparation of Cells for High-resolution Respirometry
3:40
High-resolution Respirometry
10:08
Results: Evaluation of the effects of Monomeric Cocoa Epicatechin or Curcumin on Intact Beta Cell Respiration
11:12
Conclusion
Transcription
The overall goal of this approach is to directly assess the respiratory function of pancreatic beta cells, under insulin stimulatory conditions. This can also be used to observe the effect of different compounds on beta cell respiratory function.
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The goal of this protocol is to measure the effect of glucose-mediated changes to mitochondrial respiration in the presence of natural compounds on intact 832/13 beta cells using high-resolution respirometry.