Determination of RhoA Localization Using Membrane/Cytosol Fractionation
6:05
Measurement of RhoA GTP Load using Small G-protein Activation Assay
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Results
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Conclusion
Transcription
The small Rho GTPase is a protein of a superfamily with diverse cellular function. For example, these proteins are involved in the regulation of cell fate, cellular response to a stress, cellular motility, and cell cycle. The activity of a small R
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Here we describe a protocol to investigate the prenylation and guanosine-5'-triphosphate (GTP)-loading of Rho GTPase. This protocol consists of two detailed methods, namely membrane fractionation and a GTPase-linked immunosorbent assay. The protocol can be used for measuring the prenylation and GTP loading of different other small GTPases.