Measurement of Silicone Stiffness with Sphere Indentation
4:39
Silicone Stiffness Calculation (Young s Modulus)
5:51
Results: Representative Microbead Images and Stiffness Values
6:42
Conclusion
Transcription
This method can be used to categorize substrates used to study key phenomena in mechanobiology, the cell migration and first generation. The main advantage of this technique is that it requires the use of a wide-field microscope, a piece of equipm
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Substrates with stiffness in the kilopascal-range are useful to study the response of cells to physiologically relevant micro-environment stiffness. Using just a widefield fluorescence microscope, the Young's modulus of soft silicone gels can be determined using an indentation with a suitable sphere.