This method can help in the start of genetically-engineered mouse models of prostate cancer and investigation of prostate cancer mechanism through in vivo and in vitro analysis. The main advantage of this technique is that it allows for a complete
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Genetically engineered mice are useful models for investigating prostate cancer mechanisms. Here we present a protocol to identify and dissect prostate lobes from a mouse urogenital system, differentiate them based on histology, and isolate and culture the primary prostate cells in vitro as spheroids for downstream analyses.