S'identifier

JoVE Journal

Environment

Author Produced

This content is Open Access.

Detection of Tilapia Lake Virus Using Conventional RT-PCR and SYBR Green RT-qPCR

26.4K Views

10:55 min

November 10th, 2018

November 10th, 2018

26,396 Views

0:00

Title

1:06

Sample Preparation

1:43

RNA Extraction

6:08

RNA Concentration Measurement

6:35

cDNA Synthesis

7:09

Real-time Polymerase Chain Reaction (qPCR)

9:27

Representative Results

10:22

Conclusion

Transcription

This protocol diagnoses Tilapia Lake Virus (TiLV) in tilapia tissues using RT-PCR methodologies. The entire method is described from tissue dissection to total RNA extraction, followed by cDNA synthesis and detection of TiLV by either conventional PCR or quantitative PCR using dsDNA binding a fluorescent binding dye.

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