This protocol allows us to monitor endogenous mitophagy complexes in human tissues such as beta cells, facilitating mitophagy research in pivotal translationally-relevant tissues. One advantage of this technique is its ability to visualize importa
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This protocol outlines a method for quantitative analysis of mitophagy protein complex formation specifically in beta cells from primary human islet samples. This technique thus allows analysis of mitophagy from limited biological material, which are crucial in precious human pancreatic beta cell samples.