Generation of Induced Neural Stem Cells from Peripheral Mononuclear Cells and Differentiation Toward Dopaminergic Neuron Precursors for Transplantation Studies
Isolation of PBMNCs (Peripheral Blood Mononuclear Cells)
3:00
Expansion of MNCs
4:12
Reprogramming of PBMNCs to iNSCs (Induced Neural Stem Cells) by SeV (Sendai Virus) Infection
7:20
Establishment of Unilateral 6-Hyroxydopamine (6-OHDA)-Lesioned Parkinson s Disease (PD) Mouse Models
9:15
Results: Induced Neural Stem Cells Differentiation Toward Dopaminergic Neuron Precursors and Treatment of Parkinson s Disease Mouse Models
11:00
Conclusion
Transcription
This protocol describes a method to reprogram blood and mononuclear cells to neural stem cells that can be used to treat neurodegenerative diseases, such as Parkinson's disease. It offers an autologous cell source to treat neurodegenerative diseas
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The protocol presents the reprogramming of peripheral blood mononuclear cells to induce neural stem cells by Sendai virus infection, differentiation of iNSCs into dopaminergic neurons, transplantation of DA precursors into the unilaterally-lesioned Parkinson's disease mouse models, and evaluation of the safety and efficacy of iNSC-derived DA precursors for PD treatment.