Staining and Phosphotungstic Acid (PTA) Preparation and Micro-Computed Tomography (microCT) Scanning
3:26
Data Reconstruction and Optimization
4:21
3D Reconstruction
5:31
Results: Representative 3D Orbicularis Retaining Ligament (ORL) Images
6:23
Conclusion
Transcription
We implement microCT with phosphotungstic acid to enhance the soft tissue contrast in the non-invasive investigation of the human orbicularis retaining ligament, a delicate structure within the orbit area. This protocol has been optimized to aid r
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Micro X-ray computed tomography is effective in obtaining three-dimensional information from undamaged human specimens but has limited success in observing soft tissues. The use of phosphotungstic acid contrast agent can resolve this issue. We implemented this contrast agent to examine human delicate fibromuscular tissues (the orbicularis retaining ligament).