DP (Dental Pulp) Dissection, Dispersion and Plating
3:24
Trigeminal Neuron Dissection, Dispersion and Plating
6:01
Results: Co-culture
7:20
Conclusion
Transcription
This protocol provides detailed outline of how to co-culture dental pulp stem cells with trigeminal neurons. With it, we can investigate multiple responses driven by their crosstalk. The main advantage of this technique is the ability to study and
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We describe the isolation, dispersion and plating of dental pulp (DP) primary cells with trigeminal (TG) neurons cultured atop overlying transwell filters. Cellular responses of DP cells can be analyzed with immunofluorescence or RNA/protein analysis. Immunofluorescence of neuronal markers with confocal microscopy permits the analysis of neurite outgrowth responses.