Deparaffinization, Rehydration, and Heat-Induced Antigen Retrieval
2:29
Immunolabeling and Autofluorescence Quenching
5:13
Neutrophil Extracellular Trap Identification
6:08
Results: Phase Contrast and Immunofluorescence Images
7:20
Conclusion
Transcription
This protocol of the parafinization followed by antigen retrieval of paraffin-embedded aortic sections can be a useful tool to study the role of neutrophilic tricellular traps in feline thrombosis. Detection of neutrophilic tricellular traps by im
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We describe a method to identify neutrophil extracellular traps (NETs) in formaldehyde-fixed and paraffin-embedded feline cardiogenic arterial thrombi using heat-induced antigen retrieval and a double immunolabeling protocol.