Pulmonary Artery (PA) Catheterization and Blood Perfusion
3:18
Tracheostomy and Lung Inflation
4:59
Casting the Vasculature
6:44
Results: Representative Successful Vascular Casting and Common Errors during Polymer Compound Infusion
8:24
Conclusion
Transcription
This protocol gives precise, step-by-step guidance for casting the pulmonary arterial vasculature in both adult and early postnatal mice. This technique enables users to fully cast the entire vascular network for visualization by a variety of meth
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The aim of this technique is ex vivo visualization of pulmonary arterial networks of early postnatal and adult mice through lung inflation and injection of a radio-opaque polymer-based compound via the pulmonary artery. Potential applications for casted tissues are also discussed.