Flushing Procedure for the Laser Module Prior to and Between Samples
2:28
Focusing and Positioning of the Laser Module
3:34
Loading Standards/Samples/Diluent into the Laser Module for Nanoparticle Tracking Analysis (NTA)
4:28
Sample Nanoparticle Tracking Analysis (NTA)
6:06
Re-Analysis of the Current Sample at Different Detection Threshold
6:40
Results: Effects of Camera Level and Detection Threshold on Measured Particle Size and Concentration of Filtered and Unfiltered Samples
8:02
Conclusion
Transcription
This protocol was developed for novice operators of the NanoSight LM10. By following the step-by-step instructions, accurate and reproducible results can be obtained. This technique helps ensure consistent results across multiple trial runs regard
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Nanoparticle tracking analysis (NTA) is a widely used method to characterize extracellular vesicles. This paper highlights NTA experimental parameters and controls plus a uniform method of analysis and characterization of samples and diluents necessary to supplement the guidelines proposed by MISEV2018 and EV-TRACK for reproducibility between laboratories.