Cell-Conditioned Medium (CCM) Preparation and Ultracentrifugation and Glucuronidase Encapsulation
3:22
Size-Exclusion Chromatography (SEC) Purification
4:18
Glucuronidase Assay
5:13
Extracellular Vesicle (EV) Lyophilization and Rehydration
6:19
EV Analysis After Storage
9:15
Results: Representative EV Storage Stability Evaluation
10:47
Conclusion
Transcription
The storage stability of extra cellular vesicles is an important parameter for their prospective therapeutic use. Our protocol provides a readily applicable tool for evaluating how storage effects the vesicles. The main advantage of our technique
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Here we present a readily applicable protocol to assess the storage stability of extracellular vesicles, a group of naturally occurring nanoparticles produced by cells. The vesicles are loaded with glucuronidase as a model enzyme and stored under different conditions. After storage, their physicochemical parameters and the activity of the encapsulated enzyme are evaluated.