Measuring the Prolonged Depolarizing Afterpotential (PDA) and the Early Receptor Potential (ERP) Using the Electroretinogram (ERG)
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\Results: Detecting Abnormalities in Photopigment Biogenesis by Measuring Photopigment Levels in Drosophila Using the Electroretinogram
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Conclusion
Transcription
This protocol is particularly suitable for genetic screen of visually defective Drosophila mutants. This is because they allow the detection for even small abnormalities in Drosophila phototransduction, particularly in photopigment levels and tier
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We present a protocol to electrophysiologically characterize bi-stable photopigments: (i) exploiting the charge displacements within the photopigment molecules following photon-absorption and their huge amount in the photoreceptors, and (ii) exploiting the absorption-spectra differences of rhodopsin and metarhodopsin photopigment states. These protocols are useful to screen for mutations affecting bi-stable photopigment systems.