Cell Spheroplasting, Lysis, and Restriction Site Restoration
3:19
Restriction Enzyme Digest and Intramolecular Ligation
5:15
Results: DLC and DLE Assay Analysis of D-Loops After DSB Induction
7:02
Conclusion
Transcription
The D-loop capture and extension assays are the first to allow unbiased and direct quantitation of the D-loop formation and extension steps during homologous recombination in mitotically growing yeast cells. This viability independent technique al
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The D-loop capture (DLC) and D-loop extension (DLE) assays utilize the principle of proximity ligation together with quantitative PCR to quantify D-loop formation, D-loop extension, and product formation at the site of an inducible double-stranded break in Saccharomyces cerevisiae.