Results: Measurement of Fluorogenic RNA Aptamer Kinetics
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Conclusion
Transcription
Studying in Vitro and cellular fluorogenic aptamer kinetics allows the user to evaluate if aptamer dye interactions are fast enough to study a process of interest in real time. The techniques presented allow for quantitative kinetic measurements b
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The protocol presents two methods to determine the kinetics of the fluorogenic RNA aptamers Spinach2 and Broccoli. The first method describes how to measure fluorogenic aptamer kinetics in vitro with a plate reader, while the second method details the measurement of fluorogenic aptamer kinetics in cells by flow cytometry.