Results: DRG Culture Neuronal Enrichment by Immunopanning
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Conclusion
Transcription
This protocol allows us to enhance the number of neurons in adult mouse dorsal root ganglia cultures. This may help determine the contribution of neuronal cells to a given response. We have added an immunopanning step to a basic DRG culture protoc
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This paper describes an immunopanning protocol for adult mouse dorsal root ganglia. By adhering antibodies to culture plates, we can negatively select and remove non-neuronal cells. We show that the cultures are enriched for neurons using this protocol, allowing for an in-depth study of neuronal responses to manipulation.