עבודה זו נתמכה בחלקו על ידי הפרס קריירה מן הקרן הלאומית למדע (BES-0,447,713) וממענק מאסילור, בינלאומי.

<ol>
	<li>Leigh, R. J., Zee, D. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> The Neurology of Eye Movements. , (2006).</li><li>Pruehsner, W. R., Enderle, J. D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+operating+version+of+the+Eye+Tracker,+a+system+to+measure+saccadic+eye+movements.">The operating version of the Eye Tracker, a system to measure saccadic eye movements.</a> Biomed Sci Instrum. 38, 113-118 (2002).</li><li>Pruehsner, W. R., Liebler, C. M., Rodriguez-Campos, F., Enderle, J. D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+Eye+Tracker+System--a+system+to+measure+and+record+saccadic+eye+movements.">The Eye Tracker System--a system to measure and record saccadic eye movements.</a> Biomed Sci Instrum. 39, 208-213 (2003).</li><li>Rufa, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Video-based+eye+tracking:+our+experience+with+Advanced+Stimuli+Design+for+Eye+Tracking+software.">Video-based eye tracking: our experience with Advanced Stimuli Design for Eye Tracking software.</a> Ann N Y Acad Sci. 1039, 575-579 (2005).</li><li>Cornelissen, F. W., Peters, E. M., Palmer, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+Eyelink+Toolbox:+eye+tracking+with+MATLAB+and+the+Psychophysics+Toolbox.">The Eyelink Toolbox: eye tracking with MATLAB and the Psychophysics Toolbox.</a> Behav Res Methods Instrum Comput. 34, 613-617 (2002).</li><li>Han, S. J., Guo, Y., Granger-Donetti, B., Vicci, V. R., Alvarez, T. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Quantification+of+heterophoria+and+phoria+adaptation+using+an+automated+objective+system+compared+to+clinical+methods.">Quantification of heterophoria and phoria adaptation using an automated objective system compared to clinical methods.</a> Ophthalmic Physiol Opt. 30, 95-107 (2010).</li><li>Kim, E. H., Granger-Donetti, B., Vicci, V. R., Alvarez, T. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+Relationship+between+Phoria+and+the+Ratio+of+Convergence+Peak+Velocity+to+Divergence+Peak+Velocity.">The Relationship between Phoria and the Ratio of Convergence Peak Velocity to Divergence Peak Velocity.</a> Invest Ophthalmol Vis Sci. , (2010).</li><li>Lee, Y. Y., Granger-Donetti, B., Chang, C., Alvarez, T. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Sustained+convergence+induced+changes+in+phoria+and+divergence+dynamics.">Sustained convergence induced changes in phoria and divergence dynamics.</a> Vision Res. 49, 2960-2972 (2009).</li><li>Maruta, J., Suh, M., Niogi, S. 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S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Saccade+and+vestibular+ocular+motor+adaptation.">Saccade and vestibular ocular motor adaptation.</a> Restor Neurol Neurosci. 28, 9-18 (2010).</li><li>Noudoost, B., Chang, M. H., Steinmetz, N. A., Moore, T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Top-down+control+of+visual+attention.">Top-down control of visual attention.</a> Curr Opin Neurobiol. 20, 183-190 (2010).</li><li>Herwig, A., Beisert, M., Schneider, W. X. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=On+the+spatial+interaction+of+visual+working+memory+and+attention:+evidence+for+a+global+effect+from+memory-guided+saccades.">On the spatial interaction of visual working memory and attention: evidence for a global effect from memory-guided saccades.</a> J Vis. 10, (2010).</li><li>McDowell, J. E., Dyckman, K. A., Austin, B. P., Clementz, B. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Neurophysiology+and+neuroanatomy+of+reflexive+and+volitional+saccades:+evidence+from+studies+of+humans.">Neurophysiology and neuroanatomy of reflexive and volitional saccades: evidence from studies of humans.</a> Brain Cogn. 68, 255-270 (2008).</li></ol>

אין ניגודי האינטרסים הכריזו.

צעדים קריטיים: צגי תנועת העין חייבים להיות מותאמים כראוי בנושא. לדוגמא, צגי הקלטת תנועת העין עובדים בטווח וחייבים להיות מותאם לנושא. אם תנועות העיניים של הנבדק חורגת הטווח, אז המערכת הופכת רוויה. על הרוויה, את אות תנועת העין אינה חו?...

A correction was made to <a href="http://www.jove.com/details.php?id=2530">VisualEyes: A Modular Software System for Oculomotor Experimentation</a>. There was an error in the authors, Eun H. Kim and Tara L. Alvarez, names. The author's names have been corrected to: Eun H. Kim and Tara L. Alvarez instead of: Eun Kim and Tara Alvarez

Erratum: VisualEyes: A Modular Software System for Oculomotor Experimentation

visualeyes: a modular software system for oculomotor experimentation

מחקרי תנועות עיניים סיפקו בסיס חזק להרכיב הבנה של איך המוח רוכש מידע חזותי בשני המוח הנורמלי ומתפקד. 1 עם זאת, פיתוח של פלטפורמה לעורר ותנועות עיני חנות יכולות לדרוש תכנות משמעותי, זמן ועלויות. מערכות רבות אינן מציעות את הגמישות לתכנת רבים גירויים עבור מגוון רחב של צרכי ניסוי. עם זאת, יש לו את מערכת VisualEyes ארכיטקטורה גמישה, המאפשרת למפעיל לבחור כל תכנית מסכי אחד או שניים לטנדם או מתנגד תנועות עיני רקע וגירוי חזית, ולעורר את עין השמאל וימין באופן עצמאי. מערכת זו יכולה להפחית באופן משמעותי את זמן פיתוח תכנות הדרוש כדי לערוך מחקר oculomotor. מערכת VisualEyes תידון בשלושה חלקים: 1) מכשיר הקלטת oculomotor לרכוש תנועת העין תגובות, 2) את תוכנת VisualEyes נכתבה בLabView, כדי ליצור מערך של גירוייםותגובות חנות כקבצי טקסט ו 3) ניתוח נתונים לא מקוונים. ניתן להקליט תנועות עיניים על ידי מספר סוגים של מכשור כגון: מערכת תמונת וידאו מערכת ימבוס מעקב, סליל חיפוש בלובן העין, או. גירויי תנועות עיניים אופייניים כגון צעדי saccadic, רמפות vergent וצעדי vergent עם התגובות המקבילה תוכלו לראות. בדו&quot;ח זה וידאו, אנחנו מדגימים את הגמישות של מערכת ליצור גירויים חזותיים רבים ותנועות עיני שיא שיכול להיות מנוצלות על ידי מדענים ורופאים בסיסיים ללמוד בריא כמו גם אוכלוסיות קליניות.

Watch this Scientific Journal Video about VisualEyes: A Modular Software System for Oculomotor Experimentation at JoVE.com

VisualEyes: A Modular Software System for Oculomotor Experimentation

שליטה עצבית ותהליכים קוגניטיביים ניתן ללמוד באמצעות תנועות עיניים. תוכנת VisualEyes מאפשרת למפעיל לתכנת גירויים על שני מסכי מחשב באופן עצמאי תוך שימוש בשפה פשוטה, scripting המותאם אישית. המערכת יכולה לעורר תנועות עיני טנדם (קפיצות האלה ומרדף חלק) או תנועות עיניים יריבה (התמיינות), או כל שילוב.

VisualEyes: מערכת המודולרית תוכנה לoculomotor ניסויים

Eye movement studies have provided a strong foundation forming an understanding of how the brain acquires visual information in both the normal and ...

visualeyes-a-modular-software-system-for-oculomotor-experimentation

Research

JoVE Journal

Neuroscience

12.6K Views.  New Jersey Institute of Technology.  שליטה עצבית ותהליכים קוגניטיביים ניתן ללמוד באמצעות תנועות עיניים. תוכנת VisualEyes מאפשרת למפעיל לתכנת גירויים על שני מסכי מחשב באופן עצמאי תוך שימוש בשפה פשוטה, scripting המותאם אישית. המערכת יכולה לעורר תנועות עיני טנדם (קפיצות האלה ומרדף חלק) א?...

Video: VisualEyes: A Modular Software System for Oculomotor Experimentation

Physiological Experimentation with the Crayfish Hindgut: A Student Laboratory Exercise

The purpose of the report is to describe dissection techniques for preparing the crayfish hindgut and to demonstrate how to make physiological recordings with a force transducer to monitor the strength of contraction. In addition, we demonstrate how to visually monitor peristaltic activity, which can be used as a bioassay for various peptides, biogenic amines and neurotransmitters. This preparation is amenable to student laboratories in physiology and for demonstrating pharmacological concepts to students. This preparation has been in use for over 100 years, and it still offers much as a model for investigating the generation and regulation of peristaltic rhythms and for describing the mechanisms underlying their modulation. The pharmacological assays and receptor sub-typing that were started over 50 years ago on the hindgut still contribute to research today. This robust preparation is well suited to training students in physiology and pharmacology.

In this report we demonstrate techniques that can be used to investigate the biology of the crayfish hindgut. We show how to dissect a crayfish abdomen and study the associated anatomy, physiology and modulation of activity. The peristaltic activity and strength of contractions are measured using a force transducer.

ניסויים פיזיולוגיים עם במעי האחורי סרטן הנהרות: תרגיל מעבדה סטודנטים

The purpose of the report is to describe dissection techniques for preparing the crayfish hindgut and to demonstrate how to make physiological recordings ...

VisioTracker, an Innovative Automated Approach to Oculomotor Analysis

Investigations into the visual system development and function necessitate quantifiable behavioral models of visual performance that are easy to elicit, robust, and simple to manipulate. A suitable model has been found in the optokinetic response (OKR), a reflexive behavior present in all vertebrates due to its high selection value. The OKR involves slow stimulus-following movements of eyes alternated with rapid resetting saccades. The measurement of this behavior is easily carried out in zebrafish larvae, due to its early and stable onset (fully developed after 96 hours post fertilization (hpf)), and benefitting from the thorough knowledge about zebrafish genetics, for decades one of the favored model organisms in this field. Meanwhile the analysis of similar mechanisms in adult fish has gained importance, particularly for pharmacological and toxicological applications.
Here we describe VisioTracker, a fully automated, high-throughput system for quantitative analysis of visual performance. The system is based on research carried out in the group of Prof. Stephan Neuhauss and was re-designed by TSE Systems. It consists of an immobilizing device for small fish monitored by a high-quality video camera equipped with a high-resolution zoom lens. The fish container is surrounded by a drum screen, upon which computer-generated stimulus patterns can be projected. Eye movements are recorded and automatically analyzed by the VisioTracker software package in real time.
Data analysis enables immediate recognition of parameters such as slow and fast phase duration, movement cycle frequency, slow-phase gain, visual acuity, and contrast sensitivity.
Typical results allow for example the rapid identification of visual system mutants that show no apparent alteration in wild type morphology, or the determination of quantitative effects of pharmacological or toxic and mutagenic agents on visual system performance.

The VisioTracker is an automated system for the quantitative analysis of visual performance of larval and small adult fish based on the recording of eye movements. It features full control over visual stimulus properties and real-time analysis, enabling high-throughput research in fields such as visual system development and function, pharmacology, neural circuit studies and sensorimotor integration.

VisioTracker, גישה אוטומטית חדשנית לניתוח oculomotor

Investigations into the visual system development and function necessitate quantifiable behavioral models of visual performance that are easy to elicit, ...

A Lightweight, Headphones-based System for Manipulating Auditory Feedback in Songbirds

Experimental manipulations of sensory feedback during complex behavior have provided valuable insights into the computations underlying motor control and sensorimotor plasticity1. Consistent sensory perturbations result in compensatory changes in motor output, reflecting changes in feedforward motor control that reduce the experienced feedback error. By quantifying how different sensory feedback errors affect human behavior, prior studies have explored how visual signals are used to recalibrate arm movements2,3 and auditory feedback is used to modify speech production4-7. The strength of this approach rests on the ability to mimic naturalistic errors in behavior, allowing the experimenter to observe how experienced errors in production are used to recalibrate motor output.
Songbirds provide an excellent animal model for investigating the neural basis of sensorimotor control and plasticity8,9. The songbird brain provides a well-defined circuit in which the areas necessary for song learning are spatially separated from those required for song production, and neural recording and lesion studies have made significant advances in understanding how different brain areas contribute to vocal behavior9-12. However, the lack of a naturalistic error-correction paradigm - in which a known acoustic parameter is perturbed by the experimenter and then corrected by the songbird - has made it difficult to understand the computations underlying vocal learning or how different elements of the neural circuit contribute to the correction of vocal errors13.
The technique described here gives the experimenter precise control over auditory feedback errors in singing birds, allowing the introduction of arbitrary sensory errors that can be used to drive vocal learning. Online sound-processing equipment is used to introduce a known perturbation to the acoustics of song, and a miniaturized headphones apparatus is used to replace a songbird's natural auditory feedback with the perturbed signal in real time. We have used this paradigm to perturb the fundamental frequency (pitch) of auditory feedback in adult songbirds, providing the first demonstration that adult birds maintain vocal performance using error correction14. The present protocol can be used to implement a wide range of sensory feedback perturbations (including but not limited to pitch shifts) to investigate the computational and neurophysiological basis of vocal learning.

We describe the design and assembly of miniaturized headphones suitable for replacing a songbird&#x2019;s natural auditory feedback with a manipulated acoustic signal. Online sound processing hardware is used to manipulate song output, introduce real-time errors in auditory feedback via the headphones, and drive vocal motor learning.

, מערכת אוזניות קלה משקל מבוססת על מניפולצית משוב שמיעתי בציפורי שיר

Experimental manipulations of sensory feedback during complex behavior have provided valuable insights into the computations underlying motor control and ...

A High Content Imaging Assay for Identification of Botulinum Neurotoxin Inhibitors

Synaptosomal-associated protein-25 (SNAP-25) is a component of the soluble NSF attachment protein receptor (SNARE) complex that is essential for synaptic neurotransmitter release. Botulinum neurotoxin serotype A (BoNT/A) is a zinc metalloprotease that blocks exocytosis of neurotransmitter by cleaving the SNAP-25 component of the SNARE complex. Currently there are no licensed medicines to treat BoNT/A poisoning after internalization of the toxin by motor neurons. The development of effective therapeutic measures to counter BoNT/A intoxication has been limited, due in part to the lack of robust high-throughput assays for screening small molecule libraries. Here we describe a high content imaging (HCI) assay with utility for identification of BoNT/A inhibitors. Initial optimization efforts focused on improving the reproducibility of inter-plate results across multiple, independent experiments. Automation of immunostaining, image acquisition, and image analysis were found to increase assay consistency and minimize variability while enabling the multiparameter evaluation of experimental compounds in a murine motor neuron system.

Botulinum neurotoxin is one of the most potent toxins among Category-A biothreat agents, yet a post-exposure therapeutic is not available. The high content imaging approach is a powerful methodology for identifying novel inhibitors as it enables multiparameter screening using biologically relevant motor neurons, the primary target of this toxin.

Assay הדמיה תוכן גבוה לזיהוי של טוקסין עצבי המעכבים

Synaptosomal-associated protein-25 (SNAP-25) is a component of the soluble NSF attachment protein receptor (SNARE) complex that is essential for synaptic ...

Design, Surface Treatment, Cellular Plating, and Culturing of Modular Neuronal Networks Composed of Functionally Inter-connected Circuits

The brain operates through the coordinated activation and the dynamic communication of neuronal assemblies. A major open question is how a vast repertoire of dynamical motifs, which underlie most diverse brain functions, can emerge out of a fixed topological and modular organization of brain circuits. Compared to in vivo studies of neuronal circuits which present intrinsic experimental difficulties, in vitro preparations offer a much larger possibility to manipulate and probe the structural, dynamical and chemical properties of experimental neuronal systems. This work describes an in vitro experimental methodology which allows growing of modular networks composed by spatially distinct, functionally interconnected neuronal assemblies. The protocol allows controlling the two-dimensional (2D) architecture of the neuronal network at different levels of topological complexity.
A desired network patterning can be achieved both on regular cover slips and substrate embedded micro electrode arrays. Micromachined structures are embossed on a silicon wafer and used to create biocompatible polymeric stencils, which incorporate the negative features of the desired network architecture. The stencils are placed on the culturing substrates during the surface coating procedure with a molecular layer for promoting cellular adhesion. After removal of the stencils, neurons are plated and they spontaneously redirected to the coated areas. By decreasing the inter-compartment distance, it is possible to obtain either isolated or interconnected neuronal circuits. To promote cell survival, cells are co-cultured with a supporting neuronal network which is located at the periphery of the culture dish. Electrophysiological and optical recordings of the activity of modular networks obtained respectively by using substrate embedded micro electrode arrays and calcium imaging are presented. While each module shows spontaneous global synchronizations, the occurrence of inter-module synchronization is regulated by the density of connection among the circuits.

This manuscript describes a protocol to grow in vitro modular networks consisting of spatially confined, functionally inter-connected neuronal circuits. A polymeric mask is used to pattern a protein layer to promote cellular adhesion over the culturing substrate. Plated neurons grow on coated areas establishing spontaneous connections and exhibiting electrophysiological activity.

עיצוב, משטח טיפול, סלולרי ציפוי, וCulturing של רשתות עצבי מודולרי מורכב ממעגלי קשרים בין פונקציונלי

The brain operates through the coordinated activation and the dynamic communication of neuronal assemblies. A major open question is how a vast repertoire ...

Analyzing Neural Activity and Connectivity Using Intracranial EEG Data with SPM Software

Measuring neural activity and connectivity associated with cognitive functions at high spatial and temporal resolutions is an important goal in cognitive neuroscience. Intracranial electroencephalography (EEG) can directly record electrical neural activity and has the unique potential to accomplish this goal. Traditionally, averaging analysis has been applied to analyze intracranial EEG data; however, several new techniques are available for depicting neural activity and intra- and inter-regional connectivity. Here, we introduce two analytical protocols we recently applied to analyze intracranial EEG data using the Statistical Parametric Mapping (SPM) software: time-frequency SPM analysis for neural activity and dynamic causal modeling of induced responses for intra- and inter-regional connectivity. We report our analysis of intracranial EEG data during the observation of faces as representative results. The results revealed that the inferior occipital gyrus (IOG) showed gamma-band activity at very early stages (110 ms) in response to faces, and both the IOG and amygdala showed rapid intra- and inter-regional connectivity using various types of oscillations. These analytical protocols have the potential to identify the neural mechanisms underlying cognitive functions with high spatial and temporal profiles.

We present two analytical protocols that can be used to analyze intracranial electroencephalography data using the Statistical Parametric Mapping (SPM) software: time-frequency statistical parametric mapping analysis for neural activity, and dynamic causal modeling of induced responses for intra- and inter-regional connectivity.

ניתוח פעילות עצבית וקישוריות באמצעות נתוני EEG תוך-גולגולתי בתוכנה SPM

Measuring neural activity and connectivity associated with cognitive functions at high spatial and temporal resolutions is an important goal in cognitive ...

Ex Vivo Oculomotor Slice Culture from Embryonic GFP-Expressing Mice for Time-Lapse Imaging of Oculomotor Nerve Outgrowth

Accurate eye movements are crucial for vision, but the development of the ocular motor system, especially the molecular pathways controlling axon guidance, has not been fully elucidated. This is partly due to technical limitations of traditional axon guidance assays. To identify additional axon guidance cues influencing the oculomotor nerve, an ex vivo slice assay to image the oculomotor nerve in real-time as it grows towards the eye was developed. E10.5 IslMN-GFP embryos are used to generate ex vivo slices by embedding them in agarose, slicing on a vibratome, then growing them in a microscope stage-top incubator with time-lapse photomicroscopy for 24-72 h. Control slices recapitulate the in vivo timing of outgrowth of axons from the nucleus to the orbit. Small molecule inhibitors or recombinant proteins can be added to the culture media to assess the role of different axon guidance pathways. This method has the advantages of maintaining more of the local microenvironment through which axons traverse, not axotomizing the growing axons, and assessing the axons at multiple points along their trajectory. It can also identify effects on specific subsets of axons. For example, inhibition of CXCR4 causes axons still within the midbrain to grow dorsally rather than ventrally, but axons that have already exited ventrally are not affected.

An ex vivo slice assay allows oculomotor nerve outgrowth to be imaged in real time. Slices are generated by embedding E10.5 IslMN:GFP embryos in agarose, slicing on a vibratome, and growing in a stage-top incubator. The role of axon guidance pathways is assessed by adding inhibitors to the culture media.

לשעבר Vivo Oculomotor ניע את התרבות של מעובריים GFP-הביע עכברים עבור זמן הדמיה של מניע העצבי החיצוני

Accurate eye movements are crucial for vision, but the development of the ocular motor system, especially the molecular pathways controlling axon ...

Isolation and Culture of Oculomotor, Trochlear, and Spinal Motor Neurons from Prenatal Islmn:GFP Transgenic Mice

Oculomotor neurons (CN3s) and trochlear neurons (CN4s) exhibit remarkable resistance to degenerative motor neuron diseases such as amyotrophic lateral sclerosis (ALS) when compared to spinal motor neurons (SMNs). The ability to isolate and culture primary mouse CN3s, CN4s, and SMNs would provide an approach to study mechanisms underlying this selective vulnerability. To date, most protocols use heterogeneous cell cultures, which can confound the interpretation of experimental outcomes. To minimize the problems associated with mixed-cell populations, pure cultures are indispensable. Here, the first protocol describes in detail how to efficiently purify and cultivate CN3s/CN4s alongside SMNs counterparts from the same embryos using embryonic day 11.5 (E11.5) IslMN:GFP transgenic mouse embryos. The protocol provides details on the tissue dissection and dissociation, FACS-based cell isolation, and in vitro cultivation of cells from CN3/CN4 and SMN nuclei. This protocol adds a novel in vitro CN3/CN4 culture system to existing protocols and simultaneously provides a pure species- and age-matched SMN culture for comparison. Analyses focusing on the morphological, cellular, molecular, and electrophysiological characteristics of motor neurons are feasible in this culture system. This protocol will enable research into the mechanisms that define motor neuron development, selective vulnerability, and disease.

Isolation and Culture of Oculomotor, Trochlear, and Spinal Motor Neurons from Prenatal Islmn:GFP Transgenic Mice

This work presents a protocol to yield homogeneous cell cultures of primary oculomotor, trochlear, and spinal motor neurons. These cultures can be used for comparative analyses of the morphological, cellular, molecular, and electrophysiological characteristics of ocular and spinal motor neurons.

בידוד ותרבות של והעושות, טרוליר, ושדרה מוטוריים נוירונים מ בטרום לידתי : עכברים הטרנסגניים

Oculomotor neurons (CN3s) and trochlear neurons (CN4s) exhibit remarkable resistance to degenerative motor neuron diseases such as amyotrophic lateral ...

Extracellular Electrophysiology in Modular Neuronal Cell Culture

אלקטרופיזיולוגיה חוץ-תאית בתרבית תאים עצבית מודולרית

מערכת השתלת בדיקה רב-תכליתית חסכונית לאלקטרופיזיולוגיה בעכברים

The DREAM Implant: A Lightweight, Modular, and Cost-Effective Implant System for Chronic Electrophysiology in Head-Fixed and Freely Behaving Mice

Chronic electrophysiological recordings in rodents have significantly improved our understanding of neuronal dynamics and their behavioral relevance. However, current methods for chronically implanting probes present steep trade-offs between cost, ease of use, size, adaptability, and long-term stability.
This protocol introduces a novel chronic probe implant system for mice called the DREAM (Dynamic, Recoverable, Economical, Adaptable, and Modular), designed to overcome the trade-offs associated with currently available options. The system provides a lightweight, modular and cost-effective solution with standardized hardware elements that can be combined and implanted in straightforward steps and explanted safely for recovery and multiple reuse of probes, significantly reducing experimental costs.
The DREAM implant system integrates three hardware modules: (1) a microdrive that can carry all standard silicon probes, allowing experimenters to adjust recording depth across a travel distance of up to 7 mm; (2) a three-dimensional (3D)-printable, open-source design for a wearable Faraday cage covered in copper mesh for electrical shielding, impact protection, and connector placement, and (3) a miniaturized head-fixation system for improved animal welfare and ease of use. The corresponding surgery protocol was optimized for speed (total duration: 2 h), probe safety, and animal welfare.
The implants had minimal impact on animals&#39; behavioral repertoire, were easily applicable in freely moving and head-fixed contexts, and delivered clearly identifiable spike waveforms and healthy neuronal responses for weeks of post-implant data collection. Infections and other surgery complications were extremely rare.
As such, the DREAM implant system is a versatile, cost-effective solution for chronic electrophysiology in mice, enhancing animal well-being, and enabling more ethologically sound experiments. Its design simplifies experimental procedures across various research needs, increasing accessibility of chronic electrophysiology in rodents to a wide range of research labs.

מחבר זרקור: הבנת חישוב עצבי נטורליסטי באמצעות מציאות מדומה ואלקטרופיזיולוגיה בבעלי חיים

Here, we introduce a lightweight, cost-effective probe implant system for chronic electrophysiology in rodents optimized for ease of use, probe recovery, experimental versatility, and compatibility with behavior.

שתל DREAM: מערכת שתלים קלה, מודולרית וחסכונית לאלקטרופיזיולוגיה כרונית בעכברים מקובעים ראש ומתנהגים בחופשיות

Chronic electrophysiological recordings in rodents have significantly improved our understanding of neuronal dynamics and their behavioral relevance. ...