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University Hospital Zurich

11 ARTICLES PUBLISHED IN JoVE

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Medicine

Implantation of Radiotelemetry Transmitters Yielding Data on ECG, Heart Rate, Core Body Temperature and Activity in Free-moving Laboratory Mice
Nikola Cesarovic 1, Paulin Jirkof 2, Andreas Rettich 2, Margarete Arras 1
1Division of Surgical Research, University Hospital Zurich, 2Institute of Laboratory Animal Science, University of Zurich

A surgical technique for implantation of commercially available telemetry transmitters used for continuous measurement of biopotential (one-lead ECG), heart rate, core body temperature and locomotor activity in freely moving mice is shown. Recommendations and protocols for post-operative care and pain relief, improving recovery, well being and survival rate are also presented.

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Bioengineering

Determination of the Transport Rate of Xenobiotics and Nanomaterials Across the Placenta using the ex vivo Human Placental Perfusion Model
Stefanie Grafmüller 1,2,3, Pius Manser 2, Harald F. Krug 2, Peter Wick 2, Ursula von Mandach 1
1Department of Obstetrics, Perinatal Pharmacology, University Hospital Zurich, 2Laboratory for Materials - Biology Interactions, EMPA Swiss Federal Laboratories for Materials Testing and Research, 3Graduate School for Cellular and Biomedical Sciences, University of Bern

The ex vivo dual recirculating human placental perfusion model can be used to investigate the transfer of xenobiotics and nanoparticles across the human placenta. In this video protocol we describe the equipment and techniques required for a successful execution of a placenta perfusion.

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Immunology and Infection

Intralymphatic Immunotherapy and Vaccination in Mice
Pål Johansen 1, Thomas M. Kündig 1
1Department of Dermatology, University Hospital Zurich

Prophylactic and therapeutic vaccination often fails to stimulate strong immune responses due to week drainage of the vaccine to lymph nodes and consequently poor involvement of immune cells. By direct injection of vaccine to lymph nodes, so-called intralymphatic injection, vaccine efficacy can be strongly improved and vaccine doses can be reduced.

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Bioengineering

Preparation of Light-responsive Membranes by a Combined Surface Grafting and Postmodification Process
Katrin Schöller 1, Lukas Baumann 1, Dirk Hegemann 2, Damien De Courten 3, Martin Wolf 3, René M. Rossi 1, Lukas J. Scherer 1
1Laboratory for Protection and Physiology, Empa, Swiss Federal Laboratories for Materials Science and Technology, 2Laboratory of Advanced Fibers, Empa, Swiss Federal Laboratories for Materials Science and Technology, 3Division of Neonatology, University Hospital Zurich

A plasma-induced polymerization procedure is described for the surface-initiated polymerization on polymer membranes. Further postmodification of the grafted polymer with photochromic substances is presented with a protocol of conducting permeability measurements of light-responsive membranes.

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Medicine

Ischemic Tissue Injury in the Dorsal Skinfold Chamber of the Mouse: A Skin Flap Model to Investigate Acute Persistent Ischemia
Yves Harder 1, Daniel Schmauss 1, Reto Wettstein 2, José T. Egaña 1, Fabian Weiss 1, Andrea Weinzierl 1, Anna Schuldt 1, Hans-Günther Machens 1, Michael D. Menger 3, Farid Rezaeian 4
1Department of Plastic Surgery and Hand Surgery, Klinikum rechts der Isar, Technische Universität München, 2Department of Plastic, Reconstructive, Aesthetic and Hand Surgery, University Hospital of Basel, 3Institute for Clinical and Experimental Surgery, University of Saarland, 4Division of Plastic and Hand Surgery, University Hospital Zurich

The window of the murine dorsal skinfold chamber presented visualizes a zone of acute persistent ischemia of a musculocutaneous flap. Intravital epi-fluorescence microscopy permits for direct and repetitive assessment of the microvasculature and quantification of hemodynamics. Morphologic and hemodynamic results can further be correlated with histological and molecular analyses.

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Medicine

Robotic Ablation of Atrial Fibrillation
Alexander Wutzler 1, Thomas Wolber 2, Wilhelm Haverkamp 1, Leif-Hendrik Boldt 1
1Department of Cardiology, Charité — Universitätsmedizin Berlin, Campus Virchow, 2Department of Cardiology, University Hospital Zurich

Pulmonary vein isolation (PVI) with an ablation catheter is a curative treatment for atrial fibrillation (AF). Robotic catheter systems aim to improve catheter steerability. Here, a procedure with a new robotic catheter system is presented. The goal of the procedure is electrical block between pulmonary vein and left atrium.

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Behavior

Investigating Motor Skill Learning Processes with a Robotic Manipulandum
Susan Leemburg 1, Maiko Iijima 1, Olivier Lambercy 2, Lauriane Nallet-Khosrofian 1, Roger Gassert 2, Andreas Luft 1
1Division of Vascular Neurology and Rehabilitation, Department of Neurology, University Hospital Zurich, 2Rehabilitation Engineering Laboratory, Department of Health Sciences and Technology, ETH Zurich

A paradigm is presented for training and analysis of an automated skilled reaching task in rats. Analysis of pulling attempts reveals distinct subprocesses of motor learning.

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Medicine

Stromal Vascular Fraction-enriched Fat Grafting for the Treatment of Symptomatic End-neuromata
Simon Zimmermann 1, Richard M. Fakin 1, Thomas Giesen 1, Pietro Giovanoli 1, Maurizio Calcagni 1
1Division of Plastic and Hand Surgery, University Hospital Zurich

The purpose of the study is to illustrate the technical procedure of stromal vascular fraction (SVF)-enriched fat grafting as a novel treatment of symptomatic end-neuromata. This technique provides advantages of both the mechanical barrier and the biological action at the cellular level by the processed and concentrated SVF.

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Developmental Biology

Reprogramming Primary Amniotic Fluid and Membrane Cells to Pluripotency in Xeno-free Conditions
Jaroslav Slamecka 1, Javier Laurini 2, Troy Shirley 1, Simon Philipp Hoerstrup 3, Benedikt Weber 3,4,5, Laurie Owen 1, Steven McClellan 1
1Mitchell Cancer Institute, University of South Alabama, 2College of Medicine, University of South Alabama, 3Institute for Regenerative Medicine, University of Zurich, 4Department of Dermatology, University Hospital Zurich, 5Center for Applied Biotechnology and Molecular Medicine (CABMM), University of Zurich - Irchel Campus

This protocol describes the reprogramming of primary amniotic fluid and membrane mesenchymal stem cells into induced pluripotent stem cells using a non-integrating episomal approach in fully chemically defined conditions. Procedures of extraction, culture, reprogramming, and characterization of the resulting induced pluripotent stem cells by stringent methods are detailed.

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Cancer Research

Desthiobiotin-Streptavidin-Affinity Mediated Purification of RNA-Interacting Proteins in Mesothelioma Cells
Jelena Kresoja-Rakic 1, Emanuela Felley-Bosco 1
1Laboratory of Molecular Oncology, Division of Thoracic Surgery, University Hospital Zurich

Desthiobiotin labeling of a synthetic 25-nucleotide RNA oligo, which contains an adenine-rich element (ARE) motif, allows specific binding of cytosolic ARE-binding protein.

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Genetics

Optimized High Quality DNA Extraction from Formalin-Fixed Paraffin-Embedded Human Atherosclerotic Lesions
Sabine Bauer 1,2,4, Jessica Pauli 1,2, Jaroslav Pelisek 1,3
1Department for Vascular and Endovascular Surgery, Klinikum rechts der Isar, Technical University Munich, 2partner site Munich Heart Alliance, Deutsches Zentrum für Herz-Kreislaufforschung (DZHK), 3Department of Vascular Surgery, University Hospital Zurich, 4Department of Experimental Cardiology, German Heart Centre, Technical University Munich

Here, we present a protocol for semi-automated DNA extraction from formalin-fixed paraffin-embedded lesions of human carotid arteries. The tissue lysis is performed without toxic xylene, which is followed by an automated DNA extraction protocol, including a second lysis step, binding of DNA to paramagnetic particles for cellulose based binding, washing steps, and DNA elution.

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