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Beijing Normal University

17 ARTICLES PUBLISHED IN JoVE

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Medicine

Immunohistochemical Staining of B7-H1 (PD-L1) on Paraffin-embedded Slides of Pancreatic Adenocarcinoma Tissue
Elaine Bigelow 1,2, Katherine M. Bever 1,2, Haiying Xu 1,2,3, Allison Yager 1, Annie Wu 1,2,4, Janis Taube 3,5, Lieping Chen 6, Elizabeth M. Jaffee 1,2,5,7,8, Robert A. Anders 1,2,5,8, Lei Zheng 1,2,4,5,7
1The Sidney Kimmel Comprehensive Cancer Center, The Johns Hopkins University School of Medicine, 2Department of Oncology, The Johns Hopkins University School of Medicine, 3Department of Dermatology, The Johns Hopkins University School of Medicine, 4Department of Surgery, Johns Hopkins University School of Medicine, 5The Sol Goldman Pancreatic Cancer Center, The Johns Hopkins University School of Medicine, 6Yale Cancer Center, Yale School of Medicine, 7The Skip Viragh Center for Pancreatic Cancer, The Johns Hopkins University School of Medicine, 8Department of Pathology, The Johns Hopkins University School of Medicine

B7-H1 (PD-L1) and its binding to PD-1 provide a major tumor-induced immunosuppressive signal in the tumor’s microenvironment. An immunohistochemical staining technique to characterize the expression and localization of B7-H1 in pancreatic adenocarcinoma is described here.

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Neuroscience

Promotion of Survival and Differentiation of Neural Stem Cells with Fibrin and Growth Factor Cocktails after Severe Spinal Cord Injury
Paul Lu 1,2, Lori Graham 2, Yaozhi Wang 2, Di Wu 2, Mark Tuszynski 1,2
1Veterans Administration Medical Center, San Diego, 2Department of Neurosciences, University of California, San Diego

Fibrin matrices containing growth factors were used to retain grafted neural stem cells into sites of complete spinal cord transection. Grafted cells completely filled the lesion cavity and differentiated into multiple neural cell types, including neurons that extended axons into host spinal cord over long distances.

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Medicine

A Preclinical Murine Model of Hepatic Metastases
Kevin C. Soares 1, Kelly Foley 2, Kelly Olino 1, Ashley Leubner 2, Skye C. Mayo 1, Ajay Jain 1, Elizabeth Jaffee 2, Richard D. Schulick 3, Kiyoshi Yoshimura 1,2, Barish Edil 1,2, Lei Zheng 1,2
1Department of Surgery, The Johns Hopkins University School of Medicine, 2Department of Oncology, The Sidney Kimmel Comprehensive Cancer Center, The Johns Hopkins University School of Medicine, 3Department of Surgery, University of Colorado Anschutz Medical Campus

A preclinical, murine model of hepatic metastases performed via a hemispleen injection technique.

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Neuroscience

Electrophysiological Method for Recording Intracellular Voltage Responses of Drosophila Photoreceptors and Interneurons to Light Stimuli In Vivo
Mikko Juusola 1,2, An Dau 2, Lei Zheng 1, Diana Rien 1,2
1National Key Laboratory of Cognitive Neuroscience and Learning, Beijing Normal University, 2Department of Biomedical Science, The University of Sheffield

Sharp microelectrodes enable accurate electrophysiological characterization of photoreceptor and visual interneuron output in living Drosophila. Here we show how to use this method to record high-quality voltage responses of individual cells to controlled light stimulation. This method is ideal for studying neural information processing in insect compound eyes.

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Behavior

Dissociation of the Confounding Influences of Expectancy and Integrative Difficulty Residing in Anomalous Sentences in Event-related Potential Studies
Yunlong Huang 1, Minghu Jiang 1, Qian Guo 2, Yuling Wang 1, Fan-Pei Gloria Yang 3
1Center for Psychology and Cognitive Science, School of Humanities, Tsinghua University, 2Department of Foreign Languages and Literatures, School of Humanities, Tsinghua University, 3Center for Cognition and Mind Sciences, Lab for Language and Cognitive Neurology, National TsingHua University

We present a protocol to dissociate the intertwining factors of integrative difficulty and unexpectedness in semantically anomalous sentences by applying multiple repetitions to enhance participant's expectancy for anomalous sentences. The dissociation helps to investigate the major contributor of elicited event-related potentials (ERP) effects such as N400 in language studies.

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JoVE Journal

Thermocapillary Convection Space Experiment on the SJ-10 Recoverable Satellite
Li Duan *1,2, Yongli Yin *3, Jia Wang *1, Qi Kang 1,2, Di Wu 1, Huan Jiang 1, Pu Zhang 1, Liang Hu 1
1National Microgravity Laboratory, Institute of Mechanics, Chinese Academy of Sciences, 2School of Engineering Sciences, University of Chinese Academy of Sciences, 3China Astronaut Research and Training Center

A protocol for the space payload design, the space experiment on thermocapillary convection, and analyses of experimental data and images are presented in this paper.

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Neuroscience

Stimulation Location Determination using a 3D Digitizer with High-Definition Transcranial Direct Current Stimulation
Wanting Chen 1, Rui Chen 1, Qinghua He 1,2,3,4,5
1Faculty of Psychology, Southwest University, 2Key Laboratory of Cognition and Personality, Ministry of Education, Southwest University, 3Southwest University Branch, Collaborative Innovation Center of Assessment toward Basic Education Quality, Beijing Normal University, 4Key Laboratory of Mental Health, Institute of Psychology, Chinese Academy of Sciences, 5Chongqing Collaborative Innovation Center for Brain Science

Presented here is a protocol to achieve higher accuracy in determination of stimulation location combining a 3D digitizer with high-definition transcranial direct current stimulation.

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Cancer Research

Comparing Metastatic Clear Cell Renal Cell Carcinoma Model Established in Mouse Kidney and on Chicken Chorioallantoic Membrane
Moe Ishihara *1, Junhui Hu *1, Xiaoyu Zhang 2, YongHyeon Choi 3, Anthony Wong 4, Celine Cano-Ruiz 5, Rongwei Zhao 6, Ping Tan 7, Jonathan L. Tso 1, Lily Wu 1,8
1Department of Molecular and Medical Pharmacology, David Geffen School of Medicine, University of California, Los Angeles, 2Department of Molecular, Cell, and Developmental Biology, University of California, Los Angeles, 3Department of Bioengineering, Hanyang University, 4Department of Ecology and Evolutionary Biology, University of California, Los Angeles, 5Department of Microbiology, Immunology, and Molecular Genetics, University of California, Los Angeles, 6School of Life Sciences, Beijing Normal University, 7Department of Urology, West China Hospital, Sichuan University, 8Department of Urology, David Geffen School of Medicine, University of California, Los Angeles

Metastatic clear cell renal cell carcinoma is a disease without a comprehensive animal model for thorough preclinical investigation. This protocol illustrates two novel animal models for the disease: the orthotopically implanted mouse model and the chicken chorioallantoic membrane model, both of which demonstrate lung metastasis resembling clinical cases.

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Behavior

Quantitative Static and Dynamic Assessment of Balance Control in Stroke Patients
Qiang Lin *1,2,3,4, Yuxin Zheng *1,2,3,4, Peixi Lian 3,4, Yuanyuan Guo 1,3,4, Haojie Huang 5, Zhiwei Luo 1,2, Wanying Li 1,2, Hongxin Chen 1,2,3,4, Sufen Li 1, Shijuan Lang 1,2,4, Yumei Chen 6, Haining Ou *1,2,3,4, Junjie Liang *1,2,3,4
1Department of Rehabilitation, The Fifth Affiliated Hospital of Guangzhou Medical University, 2The Rehabilitation Medicine Lab of Guangzhou Medical University, 3Experiment Education Model Center of Rehabilitation Medicine, Guangzhou Medical University, 4Department of Rehabilitation Therapy, Guangzhou Medical University, 5College of Physical Education and Sports, Beijing Normal University, 6Department of Rehabilitation, Nanfang Hospital, Southern Medical University

Presented here is a quantitative, clinical balance assessment method suitable for stroke patients with balance disorders.

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JoVE Journal

Rapid Determination of Antibody-Antigen Affinity by Mass Photometry
Di Wu 1, Grzegorz Piszczek 1
1Biophysics Core Facility, National Heart, Lung, and Blood Institute, National Institutes of Health

We describe a single-molecule approach to antigen-antibody affinity measurements using mass photometry (MP). The MP-based protocol is fast, accurate, uses a very small amount of material, and does not require protein modification.

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Behavior

An Assessment Method and Toolkit to Evaluate Keyboard Design on Smartphones
Yincheng Wang 1, Ke Wang 1, Yuqi Huang 1, Di Wu 2, Jian Wu 3, Jibo He 1
1Department of Psychology, School of Social Sciences, Tsinghua University, 2Department of Computer Science, Beijing Normal University, 3Haier Innovation Design Center, Haier Company

The presented protocol integrates various evaluation methods and demonstrates a method to evaluate the keyboard design on smartphones. Pairs matched by English characters are proposed as the input material, and the transition time between two keys is used as the dependent variable.

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Biology

Chemical Dimerization-Induced Protein Condensates on Telomeres
Rongwei Zhao 1, David M. Chenoweth 2, Huaiying Zhang 1
1Department of Biological Sciences, Mellon College of Science, Carnegie Mellon University, 2Department of Chemistry, School of Arts and Sciences, University of Pennsylvania

This protocol illustrates a chemically induced protein dimerization system to create condensates on chromatin.  The formation of promyelocytic leukemia (PML) nuclear body on telomeres with chemical dimerizers is demonstrated. Droplet growth, dissolution, localization and composition are monitored with live cell imaging, immunofluorescence (IF) and fluorescence in situ hybridization (FISH).

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Behavior

Interaction between Phonological and Semantic Processes in Visual Word Recognition using Electrophysiology
Yuling Wang 1, Minghu Jiang 1, Xinyi Xu 1, Yunlong Huang 2
1Center for Psychology and Cognitive Science, Lab of Computational Linguistics, Tsinghua University, 2Advanced Innovation Center for Future Education, Beijing Normal University

We present a protocol to explore the relative activation sequence of phonology and semantics in visual word recognition. The results show that consistent with interactive accounts, semantic and phonological representations may be processed interactively, and higher-level linguistic representations may affect early processing.

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JoVE Core

Measurement of the Directional Information Flow in fNIRS-Hyperscanning Data using the Partial Wavelet Transform Coherence Method
Siyuan Zhou *1, Yuhang Long *2, Chunming Lu 1
1State Key Laboratory of Cognitive Neuroscience and Learning & IDG/McGovern Institute for Brain Research, Beijing Normal University, 2Institute of Developmental Psychology, Faculty of Psychology, Beijing Normal University

This protocol describes partial wavelet transform coherence (pWTC) for calculating the time-lagged pattern of interpersonal neural synchronization (INS) to infer the direction and temporal pattern of information flow during social interaction. The effectiveness of pWTC in removing the confounds of signal autocorrelation on INS was proved by two experiments.

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Biochemistry

Purification of Ubiquitinated p53 Proteins from Mammalian Cells
Di Wu 1, Mengfan He 1, Dawei Li 1
1Center for Translational Medicine, The Affiliated Zhangjiagang Hospital of Soochow University

The protocol describes a step-by-step method to purify ubiquitinated proteins from mammalian cells using the p53 tumor suppressor protein as an example. Ubiquitinated p53 proteins were purified from cells under stringent nondenaturing and denaturing conditions.

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Medicine

Augmented Reality Navigation-Guided Core Decompression for Osteonecrosis of Femoral Head
Qi Wang *1,3, Qiuyuan Wang *2,3, Ran Ding 3, Youjie Yao 4, Junjun Pan 5, Weiguo Wang 3
1China-Japan Friendship School of Clinical Medicine, Peking University, 2Graduate School of Beijing University of Chinese Medicine, 3Department of Orthopedic Surgery, Beijing Key Lab of Immunes-Mediated Inflammatory Disease, China-Japan Friendship Hospital, 4Beijing Normal University, 5State Key Laboratory of Virtual Reality Technology and Systems, Beihang University

Augmented reality technology was applied to core decompression for osteonecrosis of the femoral head to realize real-time visualization of this surgical procedure. This method can effectively improve the safety and precision of core decompression.

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Behavior

Evaluating Flight Performance and Eye Movement Patterns Using Virtual Reality Flight Simulator
Luoma Ke 1, Zhejun Zhang 2, Yidan Ma 1, Yi Xiao 3, Shengjun Wu 4, Xiuchao Wang 4, Xufeng Liu 4, Jibo He 1
1Psychology Department, School of Social Sciences, Tsinghua University, 2Faculty of Psychology, Beijing Normal University, 3National Key Laboratory of Human Factors Engineering, the China Astronaut Research and Training Center, 4Department of Military Medical Psychology, Air Force Medical University

A new virtual reality flight simulator was built, which enables efficient and low-cost evaluation of flight performance and eye movement patterns. It also provides a high-potential research tool for ergonomics and other research.

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