Nagoya University Graduate School of Medicine

7 ARTICLES PUBLISHED IN JoVE

Cancer Research

Murine Experimental Model of Original Tumor Development and Peritoneal Metastasis via Orthotopic Inoculation with Ovarian Carcinoma Cells

Yoshihiro Koya^1,2, Hiroaki Kajiyama², Wenting Liu^1,2, Kiyosumi Shibata², Takeshi Senga³, Fumitaka Kikkawa²

¹Bell Research Center for Reproductive Health and Cancer, ²Department of Obstetrics and Gynecology, Nagoya University Graduate School of Medicine, ³Department of Cancer Biology, Nagoya University Graduate School of Medicine

To clarify the multistep process of peritoneal dissemination of ovarian carcinoma, the current study presents a murine experimental model of original tumor development and peritoneal metastasis via orthotopic inoculation with these tumor cells.

Biology

Use of Anti-phospho-girdin Antibodies to Visualize Intestinal Tuft Cells in Free-Floating Mouse Jejunum Cryosections

Yuka Mizutani¹, Daisuke Kuga², Machiko Iida¹, Kaori Ushida³, Tsuyoshi Takagi¹, Yoshihito Tokita¹, Masahide Takahashi³, Masato Asai^1,3

¹Division of Perinatology, Institute for Developmental Research, Aichi Human Service Center, ²Surgery Department, Anjo Kosei Hospital, ³Department of Pathology, Nagoya University Graduate School of Medicine

Kuga et al. discovered that phosphorylation-status specific antibodies against the actin binding protein girdin phosphorylated at tyrosine 1798 (pY1798) can be used to label tuft cells (TCs). This protocol allows robust visualization of TCs using immunofluorescent staining of free-floating jejunum cryosections with pY1798 antibodies.

Developmental Biology

Ovarian Tissue Culture to Visualize Phenomena in Mouse Ovary

Kouji Komatsu¹, Akira Iwase^2,3, Tomohiko Murase², Satoru Masubuchi¹

¹Department of Physiology, Aichi Medical University, ²Department of Obstetrics and Gynecology, Nagoya University Graduate School of Medicine, ³Department of Maternal and Perinatal Medicine, Nagoya University Hospital

Ovarian tissue cultures can be used as models of follicle development, ovulation, and follicle atresia and indicate regulatory mechanisms of dynamic ovarian processes.

Developmental Biology

A Mouse Distraction Osteogenesis Model

Masahito Fujio¹, Yusuke Osawa², Masaki Matsushita², Kota Ogisu¹, Shuhei Tsuchiya¹, Hiroshi Kitoh², Hideharu Hibi¹

¹Department of Oral and Maxillofacial Surgery, Nagoya University Graduate School of Medicine, ²Department of Orthopaedic Surgery, Nagoya University Graduate School of Medicine

We present a mouse tibial distraction osteogenesis model developed using a custom-made distractor. The use of a mouse as an analysis target is advantageous for advancing research.

Medicine

Near Infrared Photoimmunotherapy for Mouse Models of Pleural Dissemination

Hirotoshi Yasui¹, Yuko Nishinaga¹, Shunichi Taki¹, Kazuomi Takahashi¹, Yoshitaka Isobe¹, Kazuhide Sato^1,2,3

¹Respiratory Medicine, Nagoya University Graduate School of Medicine, ²Nagoya University Institute for Advanced Research, ³B3-Unit, Advanced Analytical and Diagnostic Imaging Center (AADIC)/Medical Engineering Unit (MEU), Nagoya University Institute for Advanced Research

Near-infrared photoimmunotherapy (NIR-PIT) is an emerging cancer therapeutic strategy that utilizes an antibody-photoabsorber (IR700Dye) conjugate and NIR light to destroy cancer cells. Here, we present a method to evaluate the antitumor effect of NIR-PIT in a mouse model of pleural disseminated lung cancer and malignant pleural mesothelioma using bioluminescence imaging.

Cancer Research

Portal Vein Injection of Colorectal Cancer Organoids to Study the Liver Metastasis Stroma

Hiroki Kobayashi^1,2,3,4, Krystyna A. Gieniec^1,2, Jia Q. Ng^1,2, Jarrad Goyne^1,2, Tamsin R. M. Lannagan^1,2, Elaine M. Thomas^1,2, Georgette Radford^1,2, Tongtong Wang^1,2, Nobumi Suzuki^1,2,5, Mari Ichinose^1,2, Josephine A. Wright², Laura Vrbanac^1,2, Alastair D. Burt⁶, Masahide Takahashi^3,4,7, Atsushi Enomoto³, Daniel L. Worthley², Susan L. Woods^1,2

¹Adelaide Medical School, University of Adelaide, ²South Australian Health and Medical Research Institute (SAHMRI), ³Department of Pathology, Nagoya University Graduate School of Medicine, ⁴Division of Molecular Pathology, Center for Neurological Disease and Cancer, Nagoya University Graduate School of Medicine, ⁵Department of Gastroenterology, Graduate School of Medicine, The University of Tokyo, ⁶Translational and Clinical Research Institute, Newcastle University, ⁷International Center for Cell and Gene Therapy, Fujita Health University

Portal vein injection of colorectal cancer (CRC) organoids generates stroma-rich liver metastasis. This mouse model of CRC hepatic metastasis represents a useful tool to study tumor-stroma interactions and develop novel stroma-directed therapeutics such as adeno-associated virus-mediated gene therapies.

Neuroscience

Evaluation and Manipulation of Neural Activity Using Two-Photon Holographic Microscopy

Daisuke Kato^1,2, Xiangyu Quan³, Yuta Tanisumi^1,2, Zhongtian Guo^1,2, Mitsuhiro Morita⁴, Tetsuya Takiguchi⁵, Osamu Matoba⁶, Hiroaki Wake^1,2,6

¹Department of Anatomy and Molecular Cell Biology, Nagoya University Graduate School of Medicine, ²Division of Multicellular Circuit Dynamics, National Institute for Physiological Sciences, National Institutes of Natural Sciences, ³Department of System Science, Kobe University Graduate School of System Informatics, ⁴Department of Biology, Graduate School of Sciences, Kobe University, ⁵Department of Information Science, Kobe University Graduate School of System Informatics, ⁶Center of Optical Scattering Image Science, Kobe University

We developed a two-photon holographic microscope that can visualize, assess, and manipulate neural activity using high spatiotemporal resolution, with the aim of elucidating the pathogenesis of neuropsychiatric disorders that are associated with abnormal neural activity.