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Nagoya University Graduate School of Medicine

7 ARTICLES PUBLISHED IN JoVE

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Cancer Research

Murine Experimental Model of Original Tumor Development and Peritoneal Metastasis via Orthotopic Inoculation with Ovarian Carcinoma Cells
Yoshihiro Koya 1,2, Hiroaki Kajiyama 2, Wenting Liu 1,2, Kiyosumi Shibata 2, Takeshi Senga 3, Fumitaka Kikkawa 2
1Bell Research Center for Reproductive Health and Cancer, 2Department of Obstetrics and Gynecology, Nagoya University Graduate School of Medicine, 3Department of Cancer Biology, Nagoya University Graduate School of Medicine

To clarify the multistep process of peritoneal dissemination of ovarian carcinoma, the current study presents a murine experimental model of original tumor development and peritoneal metastasis via orthotopic inoculation with these tumor cells.

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Biology

Use of Anti-phospho-girdin Antibodies to Visualize Intestinal Tuft Cells in Free-Floating Mouse Jejunum Cryosections
Yuka Mizutani 1, Daisuke Kuga 2, Machiko Iida 1, Kaori Ushida 3, Tsuyoshi Takagi 1, Yoshihito Tokita 1, Masahide Takahashi 3, Masato Asai 1,3
1Division of Perinatology, Institute for Developmental Research, Aichi Human Service Center, 2Surgery Department, Anjo Kosei Hospital, 3Department of Pathology, Nagoya University Graduate School of Medicine

Kuga et al. discovered that phosphorylation-status specific antibodies against the actin binding protein girdin phosphorylated at tyrosine 1798 (pY1798) can be used to label tuft cells (TCs). This protocol allows robust visualization of TCs using immunofluorescent staining of free-floating jejunum cryosections with pY1798 antibodies.

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Developmental Biology

Ovarian Tissue Culture to Visualize Phenomena in Mouse Ovary
Kouji Komatsu 1, Akira Iwase 2,3, Tomohiko Murase 2, Satoru Masubuchi 1
1Department of Physiology, Aichi Medical University, 2Department of Obstetrics and Gynecology, Nagoya University Graduate School of Medicine, 3Department of Maternal and Perinatal Medicine, Nagoya University Hospital

Ovarian tissue cultures can be used as models of follicle development, ovulation, and follicle atresia and indicate regulatory mechanisms of dynamic ovarian processes.

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Developmental Biology

A Mouse Distraction Osteogenesis Model
Masahito Fujio 1, Yusuke Osawa 2, Masaki Matsushita 2, Kota Ogisu 1, Shuhei Tsuchiya 1, Hiroshi Kitoh 2, Hideharu Hibi 1
1Department of Oral and Maxillofacial Surgery, Nagoya University Graduate School of Medicine, 2Department of Orthopaedic Surgery, Nagoya University Graduate School of Medicine

We present a mouse tibial distraction osteogenesis model developed using a custom-made distractor. The use of a mouse as an analysis target is advantageous for advancing research.

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Medicine

Near Infrared Photoimmunotherapy for Mouse Models of Pleural Dissemination
Hirotoshi Yasui 1, Yuko Nishinaga 1, Shunichi Taki 1, Kazuomi Takahashi 1, Yoshitaka Isobe 1, Kazuhide Sato 1,2,3
1Respiratory Medicine, Nagoya University Graduate School of Medicine, 2Nagoya University Institute for Advanced Research, 3B3-Unit, Advanced Analytical and Diagnostic Imaging Center (AADIC)/Medical Engineering Unit (MEU), Nagoya University Institute for Advanced Research

Near-infrared photoimmunotherapy (NIR-PIT) is an emerging cancer therapeutic strategy that utilizes an antibody-photoabsorber (IR700Dye) conjugate and NIR light to destroy cancer cells. Here, we present a method to evaluate the antitumor effect of NIR-PIT in a mouse model of pleural disseminated lung cancer and malignant pleural mesothelioma using bioluminescence imaging.

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Cancer Research

Portal Vein Injection of Colorectal Cancer Organoids to Study the Liver Metastasis Stroma
Hiroki Kobayashi 1,2,3,4, Krystyna A. Gieniec 1,2, Jia Q. Ng 1,2, Jarrad Goyne 1,2, Tamsin R. M. Lannagan 1,2, Elaine M. Thomas 1,2, Georgette Radford 1,2, Tongtong Wang 1,2, Nobumi Suzuki 1,2,5, Mari Ichinose 1,2, Josephine A. Wright 2, Laura Vrbanac 1,2, Alastair D. Burt 6, Masahide Takahashi 3,4,7, Atsushi Enomoto 3, Daniel L. Worthley 2, Susan L. Woods 1,2
1Adelaide Medical School, University of Adelaide, 2South Australian Health and Medical Research Institute (SAHMRI), 3Department of Pathology, Nagoya University Graduate School of Medicine, 4Division of Molecular Pathology, Center for Neurological Disease and Cancer, Nagoya University Graduate School of Medicine, 5Department of Gastroenterology, Graduate School of Medicine, The University of Tokyo, 6Translational and Clinical Research Institute, Newcastle University, 7International Center for Cell and Gene Therapy, Fujita Health University

Portal vein injection of colorectal cancer (CRC) organoids generates stroma-rich liver metastasis. This mouse model of CRC hepatic metastasis represents a useful tool to study tumor-stroma interactions and develop novel stroma-directed therapeutics such as adeno-associated virus-mediated gene therapies.

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Neuroscience

Evaluation and Manipulation of Neural Activity Using Two-Photon Holographic Microscopy
Daisuke Kato 1,2, Xiangyu Quan 3, Yuta Tanisumi 1,2, Zhongtian Guo 1,2, Mitsuhiro Morita 4, Tetsuya Takiguchi 5, Osamu Matoba 6, Hiroaki Wake 1,2,6
1Department of Anatomy and Molecular Cell Biology, Nagoya University Graduate School of Medicine, 2Division of Multicellular Circuit Dynamics, National Institute for Physiological Sciences, National Institutes of Natural Sciences, 3Department of System Science, Kobe University Graduate School of System Informatics, 4Department of Biology, Graduate School of Sciences, Kobe University, 5Department of Information Science, Kobe University Graduate School of System Informatics, 6Center of Optical Scattering Image Science, Kobe University

We developed a two-photon holographic microscope that can visualize, assess, and manipulate neural activity using high spatiotemporal resolution, with the aim of elucidating the pathogenesis of neuropsychiatric disorders that are associated with abnormal neural activity.

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