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Shanghai Jiao Tong University School of Medicine

30 ARTICLES PUBLISHED IN JoVE

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Biology

Generation of RNA/DNA Hybrids in Genomic DNA by Transformation using RNA-containing Oligonucleotides
Ying Shen 1, Francesca Storici 1
1School of Biology, Georgia Institute of Technology

This work shows how to form an RNA/DNA hybrid at the chromosomal level and reveal transfer of genetic information from RNA to genomic DNA in yeast cells.

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Biology

Rapid and Efficient Generation of Neurons from Human Pluripotent Stem Cells in a Multititre Plate Format
Miao Zhang 1, Hans R. Schöler 1,2, Boris Greber 1
1Max Planck Institute for Molecular Biomedicine, 2Medical Faculty, University of Münster

Protocols for neuronal differentiation of pluripotent human stem cells (hPSCs) are often time-consuming and require substantial cell culture skills. Here, we have adapted a small molecule-based differentiation procedure to a multititre plate format, allowing simple, rapid, and efficient generation of human neurons in a controlled manner.

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Immunology and Infection

Generation of a Novel Dendritic-cell Vaccine Using Melanoma and Squamous Cancer Stem Cells
Qiao Li 1, Lin Lu 1, Huimin Tao 1, Carolyn Xue 1, Seagal Teitz-Tennenbaum 2, John H. Owen 3, Jeffrey S Moyer 3, Mark E.P. Prince 3, Alfred E. Chang 1, Max S. Wicha 2
1Department of Surgery, University of Michigan, 2Department of Internal Medicine, University of Michigan, 3Department of Otolaryngology, University of Michigan

Evaluated in syngeneic immunocompetent hosts, cancer stem cell (CSC) based dendritic cell (DC) vaccine demonstrated significantly higher antitumor immunity than traditional DC vaccines pulsed with heterogeneous bulk tumor cells.

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Developmental Biology

A 5-mC Dot Blot Assay Quantifying the DNA Methylation Level of Chondrocyte Dedifferentiation In Vitro
Zhaofeng Jia 1,2, Yujie Liang 3, Bin Ma 4,5, Xiao Xu 2,6, Jianyi Xiong 2, Li Duan 2, Daping Wang 1,2
1Guangzhou Medical University, 2Shenzhen Key Laboratory of Tissue Engineering, Shenzhen Laboratory of Digital Orthopeadic Engineering, Department of Orthopedics, Shenzhen Second People's Hospital (The First Hospital Affiliated to Shenzhen University), 3Department of Chemistry, The Chinese University of Hong Kong, 4School of Biomedical Engineering, Shanghai Jiao Tong University, 5Renji Hospital Clinical Stem Cell Research Center, Shanghai Jiao Tong University School of Medicine, 6Shantou University Medical College

We present a method to quantify DNA methylation based on the 5-methylcytosine (5-mC) dot blot. We determined the 5-mC levels during chondrocyte dedifferentiation. This simple technique could be used to quickly determine the chondrocyte phenotype in ACI treatment.

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Neuroscience

Live-cell Measurement of Odorant Receptor Activation Using a Real-time cAMP Assay
Yuetian Zhang *1, Yi Pan *1, Hiroaki Matsunami 2,3, Hanyi Zhuang 1,4
1Department of Pathophysiology, Key Laboratory of Cell Differentiation and Apoptosis of National Ministry of Education, Shanghai Jiao Tong University School of Medicine, 2Department of Molecular Genetics and Microbiology, Duke University Medical Center, 3Department of Neurobiology, Duke Institute for Brain Sciences, Duke University Medical Center, 4Institute of Health Science, Chinese Academy of Science/Shanghai Jiao Tong University School of Medicine

Characterizing the function of odorant receptors serves an indispensable part in the deorphanization process. We describe a method to measure the activation of odorant receptors in real time using a cAMP assay.

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Bioengineering

Microfluidic Bioprinting for Engineering Vascularized Tissues and Organoids
Yu Shrike Zhang *1, Qingmeng Pi *1,2, Anne Metje van Genderen 1,3
1Division of Engineering in Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, 2Department of Plastic and Reconstructive Surgery, Renji Hospital, Shanghai Jiao Tong University School of Medicine, 3Division of Pharmacology, Utrecht Institute for Pharmaceutical Sciences, Utrecht University

We provide a generalized protocol based on a microfluidic bioprinting strategy for engineering a microfibrous vascular bed, where a secondary cell type could be further seeded into the interstitial space of this microfibrous structure to generate vascularized tissues and organoids.

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JoVE Journal

Gene Regulation and Targeted Therapy in Gastric Cancer Peritoneal Metastasis: Radiological Findings from Dual Energy CT and PET/CT
Bowen Shi 1, Huimin Lin 1, Miao Zhang 2, Wei Lu 3, Ying Qu 4, Huan Zhang 1
1Department of Radiology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, 2Department of Nuclear Medicine, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, 3GE Healthcare China, 4Department of Surgery, Cedars-Sinai Medical Center

This protocol describes the value of dual energy CT and PET/CT imaging methods in tumor imaging and efficacy evaluation. This article demonstrates the research methods and results acquired by dual energy CT and PET/CT to evaluate the gene regulation and targeted treatment of gastric cancer peritoneal metastasis.

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Biochemistry

Capturing the Interaction Kinetics of an Ion Channel Protein with Small Molecules by the Bio-layer Interferometry Assay
Bo Han 1, Man Zhang 2, Peng Sun 1, Shangwei Hou 3
1Department of General Surgery, Tongren Hospital, Shanghai Jiao Tong University School of Medicine, 2Key Laboratory of Systems Biomedicine (Ministry of Education), Institute of Systems Biomedicine, Shanghai Jiao Tong University, 3Hongqiao International Institute of Medicine, Tongren Hospital, Shanghai Jiao Tong University School of Medicine

The protocol here describes the interactions of purified hEAG1 ion channel protein with the small molecule lipid ligand phosphatidylinositol 4, 5-bisphosphate (PIP2). The measurement demonstrates that BLI could be a potential method for novel small-molecule ion channel ligand screening.

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Laparoscopic Low Anterior Resection with Total Mesorectal Excision for Rectal Cancer
Hao Feng *1,3, Zhi-hai Mao *1, Wen-qing Feng 1, Jing-kun Zhao 1, Cong-cong Zhu 1, Xiao-Hui Shen 2, Wolfgang E. Thasler 3, Ai-guo Lu 1, Min-hua Zheng 1
1Department of Gastrointestinal Surgery, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, 2Department of exclusive medical care center, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, 3Department of General and Visceral Surgery, Red Cross Hospital of Munich

Laparoscopic low anterior resection with total mesorectum excision is a common procedure used in the treatment of stage I-III rectal cancers. The aim of this work is to describe a laparoscopic low anterior resection approach to remove the part of the rectum containing rectal cancer.

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Unicellular Selection of Living Cells in Liquid Medium Using Laser Microdissection and a Pressure Catapulting System
Diaddin Hamdan 1, Guillaume Gapihan 1, Morad El Bouchtaoui 1, Wang Li 1,2, Anne Janin *1,3, Guilhem Bousquet *1,4,5, Christophe Leboeuf *1
1U1165, Université Paris-Diderot, INSERM, 2Pôle de Recherches Sino-Français en Science du Vivant et Génomique, Shanghai Rui Jin Hospital, Shanghai Jiao Tong University School of Medicine, 3Laboratoire de Pathologie, AP-HP-Hôpital Saint-Louis, 4Service d’Oncologie Médicale, AP-HP-Hôpital Avicenne, 5Université Paris 13

Here we describe a protocol for the use of laser microdissection and pressure catapulting to isolate living stem cells in an autophagic state, in a way that preserves their viability and tumorigenesis potential.

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Biology

Using En Face Immunofluorescence Staining to Observe Vascular Endothelial Cells Directly
Chang Li *1,2, Zhu Hui Liu *1,2, Jia Wei Chen 1,2, Xin Yi Shu 1,2, Ying Shen 1, Feng Hua Ding 1, Rui Yan Zhang 1, Wei Feng Shen 1, Lin Lu 1, Xiao Qun Wang 1
1Department of Cardiology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, 2Institute of Cardiovascular Diseases, Shanghai Jiao Tong University

Here, we present a protocol for immunofluorescence staining to observe the endothelial cells of the mouse aorta directly. This technique is useful when studying the cellular and molecular phenotype of endothelial cells in different flow patterns and in the development of atherosclerosis.

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Biochemistry

A Colorimetric Assay of Citrate Synthase Activity in Drosophila Melanogaster
Ping Wei *1, Qihong Liu *2, Wen Xue 2, Jiwu Wang 2
1Shanghai Diabetes Institute, Shanghai Key Laboratory of Diabetes Mellitus, Shanghai Clinical Center for Diabetes, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, 2Department of Anatomy of Physiology, Shanghai Jiao Tong University School of Medicine

We present a protocol for a colorimetric assay of citrate synthase activity for quantification of intact mitochondrial mass in Drosophila tissue homogenates.

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Developmental Biology

Isolation, Culture, and Adipogenic Induction of Neural Crest Original Adipose-Derived Stem Cells from Periaortic Adipose Tissue
Yiding Qi *1, Xiang Miao *2, Lian Xu 3, Mengxia Fu 3, Shi Peng 4, Kailei Shi 5, Jun Li 4, Maoqing Ye 5, Ruogu Li 1
1Department of Cardiology, Shanghai Chest Hospital, Shanghai Jiao Tong University, 2Shanghai Institute of Nutrition and Health, Shanghai Institute for Biological Sciences, Chinese Academy of Sciences, 3Shanghai Key Laboratory of Hypertension, Shanghai Institute of Hypertension, Ruijin Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, 4Department of Cardiology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, 5Department of Cardiology, Shanghai Key Laboratory of Clinical Geriatric Medicine, Huadong Hospital Affiliated to Fudan University

We present a protocol for the isolation, culture, and adipogenic induction of neural crest derived adipose-derived stem cells (NCADSCs) from the periaortic adipose tissue of Wnt-1 Cre+/-;Rosa26RFP/+ mice. The NCADSCs can be an easily accessible source of ADSCs for modeling adipogenesis or lipogenesis in vitro.

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Bioengineering

Cerebral Blood Flow-Based Resting State Functional Connectivity of the Human Brain using Optical Diffuse Correlation Spectroscopy
Chien Poon 1, Ben Rinehart 1, Jun Li 1,2, Ulas Sunar 1
1Department of Biomedical, Industrial and Human Factors Engineering, Wright State University, 2South China Academy of Advanced Optoelectronics, South China Normal University

This protocol demonstrates how to measure resting state functional connectivity in the human prefrontal cortex using a custom-made diffuse correlation spectroscopy instrument. The report also discuss practical aspects of the experiment as well as detailed steps for analyzing the data.

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Immunology and Infection

Flow Cytometric Analysis of Lymphocyte Infiltration in Central Nervous System during Experimental Autoimmune Encephalomyelitis
Zhe Ji 1,2, Chenghua Zhou 1, Hongshen Niu 3, Jinshen Wang 1, Lei Shen 3
1Translational Medicine Research Center, Ruijin Hospital North, Shanghai Jiao Tong University School of Medicine, 2Department of Microbiology and Immunology, Tongji University School of Medicine, 3Shanghai Institute of Immunology, Shanghai Key Laboratory for Tumor Microenvironment and Inflammation, Shanghai Jiao Tong University School of Medicine

This manuscript presents a protocol to induce active experimental autoimmune encephalomyelitis (EAE) in mice. A method for the isolation and characterization of the infiltrated lymphocytes in the central nervous system (CNS) is also presented to show how lymphocytes are involved in the development of CNS autoimmune disease.

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Neuroscience

Primary Microglia Isolation from Postnatal Mouse Brains
Siling Du *1,2, Shanshan Xiong *3, Xiangjuan Du 4, Ti-Fei Yuan 1, Bo Peng 3,4,5, Yanxia Rao 1
1Shanghai Key Laboratory of Psychotic Disorders, Shanghai Mental Health Center, Shanghai Jiao Tong University School of Medicine, 2Division of Biology and Biomedical Science, Washington University in St. Louis, 3Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, 4Department of Neurosurgery, Huashan Hospital, Institute for Translational Brain Research, State Key Laboratory of Medical Neurobiology, MOE Frontiers Center for Brain Science, Fudan University, 5Co-innovation Center of Neuroregeneration, Nantong University

Primary cell culture is one of the primarily used approaches for studying microglial biology in vitro. Here, we developed a method for simple and rapid microglia isolation from the mouse postnatal day 1 (P1) to P4.

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JoVE Journal

Extracellular Vesicle Uptake Assay via Confocal Microscope Imaging Analysis
Chi-Ju Kim *1,2, Morgan D. Kuczler *1, Liang Dong 1,3, Junyoung Kim 2,4, Sarah R. Amend 1, Yoon-Kyoung Cho 2,4, Kenneth J. Pienta 1
1The Brady Urological Institute, Johns Hopkins University School of Medicine, 2Department of Biomedical Engineering, School of Life Sciences, Ulsan National Institute of Science and Technology (UNIST), 3Department of Urology, Renji Hospital, Shanghai Jiao Tong University School of Medicine, 4Center for Soft and Living Matter, Institute for Basic Science (IBS)

Extracellular vesicles (EVs) contribute to cellular biology and intercellular communications. There is a need for practical assays to visualize and quantify EVs uptake by the cells. The current protocol proposes the EV uptake assay by utilizing three-dimensional fluorescence imaging via confocal microscopy, following EV isolation by a nano-filtration-based microfluidic device.

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Medicine

A Rat Orthotopic Renal Transplantation Model for Renal Allograft Rejection
Hang You 1,2, Xin Mao 2, Chenyang Wang 2, Gang Huang 3, Marcus Groettrup 4,5, Jun Li 2
1School of Medicine, Chongqing University, 2Department of Urological Oncology Surgery, Chongqing University Cancer Hospital, 3Department of Biochemistry and Molecular Biology, College of Basic Medical Science, Army Medical University, 4Division of Immunology, Department of Biology, University of Konstanz, 5Biotechnology Institute Thurgau, University of Konstanz

The rat orthotopic renal transplantation model contributes to investigating the mechanism of renal allograft rejection. The current model increases the recipients' survival without interference with blood supply and venous reflux of the lower body using an end-to-end anastomosis of kidney implantation and an end-to-side "tunnel" method of ureter-bladder anastomosis.

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Medicine

A Modified Cuff Technique for Mouse Cervical Heterotopic Heart Transplantation Model
Xin Mao *1, Peng Xian *1, Hang You 1,2, Gang Huang 3, Jun Li 1
1Department of Urological Oncology Surgery, Chongqing University Cancer Hospital, 2School of Medicine, Chongqing University, 3Department of Biochemistry and Molecular Biology, College of Basic Medical Science, Army Medical University

In the present protocol, a mouse heart transplantation model is used for investigating the mechanism of cardiac allograft rejection. In this heterotopic heart transplantation model, operation efficiency is improved, and the survival of cardiac grafts is ensured by a cervical end-to-end anastomosis of heart implantation using a modified Cuff technique.

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Medicine

Microscopic Replantation of Penile Glans Amputation Due to Circumcision
Da-Chun Jin *1, Bo Zhou *1, Jun Li 2, Cong-Cong Bao 1, Yong Luo 1, Yong Zhang 1, Peng Wang 1, Gang Bi 1, Yan-Feng Li 1
1Department of Urology, Daping Hospital, Army Medical University, 2Department of Urology, Chongqing 13th People's Hospital

The present protocol describes the emergency management of microscopic replantation of penile glans amputation due to circumcision.

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Bioengineering

Construction of a Human Aorta Smooth Muscle Cell Organ-On-A-Chip Model for Recapitulating Biomechanical Strain in the Aortic Wall
Mieradilijiang Abudupataer *1, Xiujie Yin *1, Bitao Xiang *1, Nan Chen 1, Shiqiang Yan 2, Shichao Zhu 1, Yang Ming 1, Gang Liu 1, Xiaonan Zhou 1, Hao Lai 1, Chunsheng Wang 1, Kai Zhu 1, Jun Li 1
1Department of Cardiac Surgery and Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital, Fudan University, 2Institutes of Biomedical Sciences and the Shanghai Key Laboratory of Medical Epigenetics, Shanghai Medical College, Fudan University

Here, we developed a human aorta smooth muscle cell organ-on-a-chip model to replicate the in vivo biomechanical strain of smooth muscle cells in the human aortic wall.

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Medicine

Isolation of Murine Intestinal Mesenchyme Resulting in a High Yield of Telocytes
Marco Canella *1, Jianmei Tan *1,2, Bing Su 2, Michal Shoshkes-Carmel 1
1Department of Developmental Biology and Cancer Research, The Institute for Medical Research Israel-Canada, The Hebrew University - Hadassah Medical School, 2Shanghai Institute of Immunology, Department of Immunology and Microbiology, Shanghai Jiao Tong University School of Medicine

Here, we present a protocol to isolate murine intestinal mesenchyme, including telocytes. These can be used for several applications, such as co-culture with mouse or human-derived organoids, to support growth and better reflect the situation in the original tissue.

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Bioengineering

IDG-SW3 Cell Culture in a Three-Dimensional Extracellular Matrix
Kaizhe Chen *1, Haoyi Chen *1, Lianfu Deng 1, Kai Yang 1, Jin Qi 1
1Department of Orthopedics, Shanghai Key Laboratory for Prevention and Treatment of Bone and Joint Diseases, Shanghai Institute of Traumatology and Orthopaedics, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine

Here, we present a protocol for culturing IDG-SW3 cells in a three-dimensional (3D) extracellular matrix.

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Medicine

Establishment and Confirmation of a Postnatal Right Ventricular Volume Overload Mouse Model
Sijuan Sun *1, Hongbin Zhu *2, Shoubao Wang 3, Xiuxia Xu 4, Lincai Ye 2,5,6
1Department of Pediatric Intensive Care Unit, Shanghai Children’s Medical Center, School of Medicine, Shanghai Jiaotong University, 2Department of Thoracic and Cardiovascular Surgery, Shanghai Children’s Hospital, School of Medicine, Shanghai Jiaotong University, 3Department of Plastic and Reconstructive Surgery, Shanghai Ninth People’s Hospital, School of Medicine, Shanghai Jiao Tong University, 4Department of Radiology, Huangpu Branch, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, 5Institute of Pediatric Translational Medicine, Shanghai Children’s Medical Center, School of Medicine, Shanghai Jiaotong University, 6Shanghai Institute for Pediatric Congenital Heart Disease, Shanghai Children’s Medical Center, School of Medicine, Shanghai Jiaotong University

This protocol presents the establishment and confirmation of a postnatal right ventricular volume overload (VO) model in mice with abdominal arteriovenous fistula (AVF), which can be applied to investigate how VO contributes to postnatal heart development.

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Medicine

Using Inducible Osteoblastic Lineage-Specific Stat3 Knockout Mice to Study Alveolar Bone Remodeling During Orthodontic Tooth Movement
Yuanqi Liu *1,2,3,4,5, Siyuan Sun *1,2,3,4,5, Ziyi Jiang *6, Xinyi Gong 1,2,3,4,5, Yiling Yang 1,2,3,4,5, Yanfei Zhu 1,2,3,4,5, Hongyuan Xu 1,2,3,4,5, Anting Jin 1,2,3,4,5, Xiangru Huang 1,2,3,4,5, Xin Gao 1,2,3,4,5, Tingwei Lu 1,2,3,4,5, Jingyi Liu 1,2,3,4,5, Xinyu Wang 1,2,3,4,5, Qinggang Dai 2,3,4,5,7, Lingyong Jiang 1,2,3,4,5
1Center of Craniofacial Orthodontics, Department of Oral and Cranio-maxillofacial Surgery, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, 2College of Stomatology, Shanghai Jiao Tong University, 3National Center for Stomatology, 4National Clinical Research Center for Oral Diseases, 5Shanghai Key Laboratory of Stomatology, 6Shanghai Starriver Bilingual School, 7The 2nd Dental Center, Ninth People’s Hospital, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine

This study provides a protocol for using inducible osteoblast lineage-specific Stat3 knockout mice to study bone remodeling under orthodontic force and describes methods for analyzing alveolar bone remodeling during orthodontic tooth movement, thus shedding light on skeletal mechanical biology.

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Biology

Isolation, Culture, and Adipogenic Induction of Stromal Vascular Fraction-derived Preadipocytes from Mouse Periaortic Adipose Tissue
Min Liang *1, Yijie Huang *1, Yangjing Jiang 1, Yunwen Hu 1, Zhaohua Cai 1, Ben He 1
1Department of Cardiology, Shanghai Chest Hospital, Shanghai Jiao Tong University School of Medicine

Here, we describe the isolation, culture, and adipogenic induction of stromal vascular fraction-derived preadipocytes from mouse periaortic adipose tissue, allowing for the study of perivascular adipose tissue function and its relationship with vascular cells.

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Biology

A 3-D Visualization Technique for Bone Remodeling in a Suture Expansion Mouse Model
Ziduan Ding 1,2, Ruomei Li 1,2, Yufeng Duan 1,2, Zhenxia Li 1,2, Bing Fang 1,2, Dian Jing 1,2
1Department of Orthodontics, China Shanghai Ninth People’s Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine, 2National Center for Stomatology, National Clinical Research Center for Oral Diseases, Shanghai Key Laboratory of Stomatology, Shanghai Jiao Tong University

This protocol presents a standardized suture expansion mouse model and a 3-D visualization method to study the mechanobiological changes of the suture and bone remodeling under tensile force loading.

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Medicine

Cryopreservation of Human Embryonic Stem Cell-Derived Retinal Pigment Epithelial Cells at the Optimal Stage
Xinyue Bai *1,2,3,4,5,6,7, Ting Zhang *1,2,3,4,5,6,7, Xinyue Zhu 1,2,3,4,5,6,7, Xianyu Huang 1,2,3,4,5,6,7, Haiyun Liu 1,2,3,4,5,6,7, Xiaoyan Ding 8, Mei Jiang 1,2,3,4,5,6,7, Xiaodong Sun 1,2,3,4,5,6,7
1Department of Ophthalmology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, 2National Clinical Research Center for Eye Diseases, 3Shanghai Clinical Research Center for Eye Diseases, 4Shanghai Key Clinical Specialty, 5Shanghai Key Laboratory of Ocular Fundus Diseases, 6Shanghai Engineering Center for Visual Science and Photomedicine, 7Shanghai Engineering Center for Precise Diagnosis and Treatment of Eye Diseases, 8Stem Cell Core Facility, CAS Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences

This study provides a detailed protocol for the efficient cryopreservation of human stem cell-derived retinal pigment epithelial cells.

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Medicine

Determining Gender-Based Differences in Retinal and Choroidal Thickness in Underweight Individuals via Swept-Source Optical Coherence Tomography
Jin Wang *1, Qianlin Ji *2, Sijie Lin *3, Yang Zhang 2, Jing Jiang 2, Yu Zhang 2, Yiwen Qian 2, Xin Che 2, Yan Liu 2, Zhiliang Wang 2, Qingjian Li 2
1Department of Cardiology, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, 2Department of Ophthalmology, Huashan Hospital, Fudan University, 3Eye Institute of Xiamen University, Xiamen University School of Medicine

Here, swept-source optical coherence tomography (SS-OCT) is used to compare retinal and choroidal thickness in adults with and without malnutrition, contributing to a better understanding of the pathogenesis of ocular diseases in malnourished individuals.

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Medicine

Early Weight-Bearing Rehabilitation Protocol After Anterior Cruciate Ligament Reconstruction
Renren Du *1, Wenjiang Sun *2, Fei He 2, Liming Jiang 3, Wengao Cheng 4, Bo Yu 2, Jiwu Chen 5
1Rehabilitation center, Shanghai Second Rehabilitation Hospital, 2Department of Rehabilitation Medicine, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, 3Rehabilitation center, The Seventh People's Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, 4Rehabilitation center, Shanghai Lanhai Rehabilitation Hospital, 5Department of Sports Medicine, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine

Based on the safety and feasibility, this article presents an early weight-bearing rehabilitation protocol after anterior cruciate ligament reconstruction. The protocol is clear and easy to operate, which is helpful to promote its use in clinical practice and accelerate the functional recovery of patients.

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