German Centre for Cardiovascular Research (DZHK)

13 ARTICLES PUBLISHED IN JoVE

Biology

Heterotopic and Orthotopic Tracheal Transplantation in Mice used as Models to Study the Development of Obliterative Airway Disease

Xiaoqin Hua¹, Tobias Deuse^1,2, Karis R. Tang-Quan^1,2,3, Robert C. Robbins³, Hermann Reichenspurner^1,2, Sonja Schrepfer^1,2,3

¹Transplant and Stem Cell Immunobiology Lab (TSI), University Heart Center Hamburg, ²CVRC, University Hospital Hamburg, ³Department of CT Surgery, Stanford University School of Medicine

This video shows and compares two experimental models to study the development of obliterative airway disease (OAD) in mice, the heterotopic and orthotopic tracheal transplantation model.

Biology

LAD-Ligation: A Murine Model of Myocardial Infarction

Mandy V.V. Kolk^1,2, Danja Meyberg^1,2, Tobias Deuse^1,2, Karis R. Tang-Quan^1,2,3, Robert C. Robbins³, Hermann Reichenspurner^1,2, Sonja Schrepfer^1,2,3

¹Transplant and Stem Cell Immunobiology Lab (TSI), University Heart Center Hamburg, ²CVRC, University Hospital Hamburg, ³Department of CT Surgery, Stanford University School of Medicine

This video demonstrates how to use a fast and reliable model to study pathobiological and pathophysiological processes of myocardial ischemia.

Medicine

Orthotopic Aortic Transplantation: A Rat Model to Study the Development of Chronic Vasculopathy

Mandy Stubbendorff¹, Tobias Deuse^1,2, Anna Hammel¹, Robert C. Robbins², Hermann Reichenspurner¹, Sonja Schrepfer^1,2

¹University Heart Center Hamburg, Transplant and Stem Cell Immunobiology Lab (TSI), University Hospital Hamburg, ²Stanford University School of Medicine

This video demonstrates the orthotopic aortic transplant model as a simple model to study the development of transplant vasculopathy (TVP) in rats.

Bioengineering

Bioluminescence Imaging for Assessment of Immune Responses Following Implantation of Engineered Heart Tissue (EHT)

Lenard Conradi^1,2, Christiane Pahrmann¹, Stephanie Schmidt¹, Tobias Deuse^1,3, Arne Hansen², Alexandra Eder², Hermann Reichenspurner¹, Robert C. Robbins³, Thomas Eschenhagen², Sonja Schrepfer^1,3

¹Transplant and Stem Cell Immunobiology Lab (TSI) and CVRC, University Hospital Hamburg, University Heart Center Hamburg, ²Department of Experimental and Clinical Pharmacology and Toxicology, University Heart Center Hamburg, ³CT Surgery, Stanford University School of Medicine

This video demonstrates the use of in vivo bioluminescence imaging to study immune responses after implantation of Engineered Heart Tissue (EHT) in rats.

Medicine

Human Internal Mammary Artery (IMA) Transplantation and Stenting: A Human Model to Study the Development of In-Stent Restenosis

Xiaoqin Hua^1,2, Tobias Deuse^1,2, Evangelos D. Michelakis³, Alois Haromy³, Phil S. Tsao⁴, Lars Maegdefessel⁴, Reinhold G. Erben⁵, Claudia Bergow⁵, Boris B. Behnisch⁶, Hermann Reichenspurner^1,2, Robert C. Robbins⁷, Sonja Schrepfer^1,2,7

¹University Heart Center Hamburg, TSI-Lab, Germany, ²Cardiovascular Research Center, University of Hamburg, ³Department of Medicine, Cardiology Division, Pulmonary Hypertension Program, University of Alberta, ⁴Department of Medicine, Stanford University School of Medicine , ⁵Department of Biomedical Sciences, Institute of Physiology, Pathophysiology, and Biophysics, University of Veterinary Medicine, Vienna, ⁶Translumina GmbH, Hechingen, ⁷Department of Cardiothoracic Surgery, Stanford University School of Medicine

This video shows a model to study the development of intimal hyperplasia after stent deployment using a human vessel (IMA) in an immunodeficient rat model.

Medicine

Inducing Myointimal Hyperplasia Versus Atherosclerosis in Mice: An Introduction of Two Valid Models

Mandy Stubbendorff^*1,2, Xiaoqin Hua^*1,2, Tobias Deuse^1,2,3, Ziad Ali^4,5, Hermann Reichenspurner^2,3, Lars Maegdefessel⁶, Robert C. Robbins⁷, Sonja Schrepfer^1,2,3,4

¹Transplant and Stem Cell Immunobiology Lab, Cardiovascular Research Center, University Hospital Hamburg, ²Cardiovascular Research Center (CVRC) and DZHK University Hamburg, ³Department of Cardiovascular Surgery, University Heart Center Hamburg, ⁴Center for Interventional Vascular Therapy, Division of Cardiology, Columbia University, ⁵Cardiovascular Research Foundation, New York, ⁶Karolinska Institute, Stockholm, ⁷Department of Cardiothoracic Surgery, Stanford University School of Medicine, Falk Cardiovascular Research Center

This video shows two models of intimal plaque development in murine arteries and emphasizes the differences in myointimal hyperplasia and atherosclerosis.

Medicine

Vein Interposition Model: A Suitable Model to Study Bypass Graft Patency

Dong Wang^1,2,3,4, Grigol Tediashvili^1,2,3, Simon Pecha⁴, Hermann Reichenspurner⁴, Tobias Deuse^1,2,3,4, Sonja Schrepfer^1,2,3,4

¹Transplant and Stem Cell Immunobiology Lab, University Heart Center Hamburg, ²Department of Surgery, Transplant and Stem Cell Immunobiology Lab, University of California San Francisco (UCSF), ³Cardiovascular Research Center (CVRC) and DZHK German Center for Cardiovascular Research), partner site Hamburg/Kiel/Luebeck, ⁴Cardiovascular Surgery, University Heart Center Hamburg

This video demonstrates a model to study the development of myointimal hyperplasia after venous interposition surgery in rats.

JoVE Core

Automated Contraction Analysis of Human Engineered Heart Tissue for Cardiac Drug Safety Screening

Ingra Mannhardt¹, Umber Saleem¹, Anika Benzin¹, Thomas Schulze¹, Birgit Klampe¹, Thomas Eschenhagen¹, Arne Hansen¹

¹Department of Experimental Pharmacology and Toxicology, Cardiovascular Research Center, University Medical Center Hamburg-Eppendorf and DZHK (German Center for Cardiovascular Research)

Here, we show the generation of human engineered heart tissue from induced pluripotent stem cells (hiPSC)-derived cardiomyocytes. We present a method to analyze contraction force and exemplary alteration of contraction pattern by the hERG channel inhibitor E-4031. This method shows high level of robustness and suitability for cardiac drug screening.

Medicine

Balloon-based Injury to Induce Myointimal Hyperplasia in the Mouse Abdominal Aorta

Grigol Tediashvili^1,2,3, Dong Wang^1,2,3,4, Hermann Reichenspurner⁴, Tobias Deuse^1,2,3,4, Sonja Schrepfer^1,2,3,4

¹Transplant and Stem Cell Immunobiology Lab, University Heart Center, ²Department of Surgery, Transplant and Stem Cell Immunobiology Lab, University of California San Francisco (UCSF), ³Cardiovascular Research Center (CVRC) and DZHK German Center for Cardiovascular Research, ⁴Cardiovascular Surgery, University Heart Center

This article demonstrates a murine model to study the development of myointimal hyperplasia (MH) after aortic balloon injury.

Biology

Miniaturized Sample Preparation for Transmission Electron Microscopy

Claudio Schmidli^*1,2, Luca Rima^*1, Stefan A. Arnold^*1,2, Thomas Stohler¹, Anastasia Syntychaki¹, Andrej Bieri¹, Stefan Albiez¹, Kenneth N. Goldie¹, Mohamed Chami³, Henning Stahlberg¹, Thomas Braun¹

¹Center for Cellular Imaging and NanoAnalytics (C-CINA), Biozentrum, University of Basel, ²Swiss Nanoscience Institute, University of Basel, ³BioEM lab, Biozentrum, University of Basel

An instrument and methods for the preparation of nanoliter-sized sample volumes for transmission electron microscopy is presented. No paper-blotting steps are required, thus avoiding the detrimental consequences this can have for proteins, significantly reducing sample loss and enabling the analysis of single cell lysate for visual proteomics.

Biochemistry

Identification of Functional Protein Regions Through Chimeric Protein Construction

Juan M. Adrian-Segarra¹, Holger Lörchner^1,2, Thomas Braun^1,2, Jochen Pöling^1,2

¹Department of Cardiac Development and Remodelling, Max Planck Institute for Heart and Lung Research, ²Partner site Rhein-Main, German Centre for Cardiovascular Research (DZHK)

Structurally related proteins frequently exert distinct biological functions. The exchange of equivalent regions of these proteins in order to create chimeric proteins constitutes an innovative approach to identify critical protein regions that are responsible for their functional divergence.

Medicine

Implantation of hiPSC-derived Cardiac-muscle Patches after Myocardial Injury in a Guinea Pig Model

Liesa Castro^1,2, Birgit Geertz³, Marina Reinsch^2,3, Bülent Aksehirlioglu³, Arne Hansen^2,3, Thomas Eschenhagen^2,3, Hermann Reichenspurner^1,2, Florian Weinberger^*2,3, Simon Pecha^*1,2

¹Department of Cardiovascular Surgery, University Heart Center Hamburg, ²partner site Hamburg/Kiel/Lübeck, German Centre for Cardiovascular Research (DZHK), ³Department of Experimental Pharmacology and Toxicology, Cardiovascular ResearchCenter, University Medical Center Hamburg-Eppendorf

Here we present a protocol for the induction of left ventricular cryoinjury followed by the implantation of a cardiac muscle patch, derived from human iPS-cell cardiomyocytes in a guinea pig model.

Developmental Biology

Magnetic Adjustment of Afterload in Engineered Heart Tissues

Benjamin Becker^*1,2, Marita L. Rodriguez^*1,2, Tessa R. Werner^1,2, Justus Stenzig^1,2, Thomas Eschenhagen^1,2, Marc N. Hirt^1,2

¹Institute of Experimental Pharmacology and Toxicology, University Medical Center Hamburg-Eppendorf, ²DZHK (German Centre for Cardiovascular Research)

This protocol provides detailed methods describing the fabrication and implementation of a magnetics-based afterload tuning platform for engineered heart tissues.