Accedi

Sigma Life Science

3 ARTICLES PUBLISHED IN JoVE

Biology

Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library

Matthew J. Coussens^*1, Kevin Forbes^*1, Carol Kreader^*1, Jack Sago¹, Carrie Cupp¹, John Swarthout¹

The Target ID Library is a plasmid-based, genome-wide collection of cloned cDNA used to identify miRNA targets. Here we demonstrate its use and application.

Biology

Genome Editing with CompoZr Custom Zinc Finger Nucleases (ZFNs)

Keith Hansen¹, Matthew J. Coussens¹, Jack Sago¹, Shilpi Subramanian¹, Monika Gjoka¹, Dave Briner¹

¹Emerging Technologies, Sigma Life Science

The CompoZr Custom Zinc-Finger Nuclease (ZFN) Service enables precise genome editing in any organism or cell line at any locus defined by the user. This article describes the process for the design, manufacture, validation and implementation of the CompoZr Custom ZFN Service.

Biology

MISSION LentiPlex Pooled shRNA Library Screening in Mammalian Cells

Matthew J. Coussens¹, Courtney Corman¹, Ashley L. Fischer¹, Jack Sago¹, John Swarthout¹

¹Sigma-Aldrich

Here we use a human LentiPlex pooled library and traditional sequencing methods to identify gene targets promoting cell survival. We demonstrate how to set up and deconvolute a LentiPlex screen and validate the results.