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University Heart Center

13 ARTICLES PUBLISHED IN JoVE

Biology

Imaging In-Stent Restenosis: An Inexpensive, Reliable, and Rapid Preclinical Model

Tobias Deuse¹, Fumiaki Ikeno², Robert C. Robbins¹, Sonja Schrepfer¹

¹Department of Cardiothoracic Surgery, Stanford University School of Medicine, ²Stanford University School of Medicine

This video demonstrates how to use a preclinical inexpensive and reliable model to study pathobiological and pathophysiological processes of in-stent restenosis development. Longitudinal in vivo monitoring using OCT (Optical Coherence Tomography) and analysis of OCT images are also demonstrated.

Biology

Heterotopic and Orthotopic Tracheal Transplantation in Mice used as Models to Study the Development of Obliterative Airway Disease

Xiaoqin Hua¹, Tobias Deuse^1,2, Karis R. Tang-Quan^1,2,3, Robert C. Robbins³, Hermann Reichenspurner^1,2, Sonja Schrepfer^1,2,3

¹Transplant and Stem Cell Immunobiology Lab (TSI), University Heart Center Hamburg, ²CVRC, University Hospital Hamburg, ³Department of CT Surgery, Stanford University School of Medicine

This video shows and compares two experimental models to study the development of obliterative airway disease (OAD) in mice, the heterotopic and orthotopic tracheal transplantation model.

Biology

LAD-Ligation: A Murine Model of Myocardial Infarction

Mandy V.V. Kolk^1,2, Danja Meyberg^1,2, Tobias Deuse^1,2, Karis R. Tang-Quan^1,2,3, Robert C. Robbins³, Hermann Reichenspurner^1,2, Sonja Schrepfer^1,2,3

¹Transplant and Stem Cell Immunobiology Lab (TSI), University Heart Center Hamburg, ²CVRC, University Hospital Hamburg, ³Department of CT Surgery, Stanford University School of Medicine

This video demonstrates how to use a fast and reliable model to study pathobiological and pathophysiological processes of myocardial ischemia.

Medicine

Orthotopic Aortic Transplantation: A Rat Model to Study the Development of Chronic Vasculopathy

Mandy Stubbendorff¹, Tobias Deuse^1,2, Anna Hammel¹, Robert C. Robbins², Hermann Reichenspurner¹, Sonja Schrepfer^1,2

¹University Heart Center Hamburg, Transplant and Stem Cell Immunobiology Lab (TSI), University Hospital Hamburg, ²Stanford University School of Medicine

This video demonstrates the orthotopic aortic transplant model as a simple model to study the development of transplant vasculopathy (TVP) in rats.

Bioengineering

Bioluminescence Imaging for Assessment of Immune Responses Following Implantation of Engineered Heart Tissue (EHT)

Lenard Conradi^1,2, Christiane Pahrmann¹, Stephanie Schmidt¹, Tobias Deuse^1,3, Arne Hansen², Alexandra Eder², Hermann Reichenspurner¹, Robert C. Robbins³, Thomas Eschenhagen², Sonja Schrepfer^1,3

¹Transplant and Stem Cell Immunobiology Lab (TSI) and CVRC, University Hospital Hamburg, University Heart Center Hamburg, ²Department of Experimental and Clinical Pharmacology and Toxicology, University Heart Center Hamburg, ³CT Surgery, Stanford University School of Medicine

This video demonstrates the use of in vivo bioluminescence imaging to study immune responses after implantation of Engineered Heart Tissue (EHT) in rats.

Medicine

Human Internal Mammary Artery (IMA) Transplantation and Stenting: A Human Model to Study the Development of In-Stent Restenosis

Xiaoqin Hua^1,2, Tobias Deuse^1,2, Evangelos D. Michelakis³, Alois Haromy³, Phil S. Tsao⁴, Lars Maegdefessel⁴, Reinhold G. Erben⁵, Claudia Bergow⁵, Boris B. Behnisch⁶, Hermann Reichenspurner^1,2, Robert C. Robbins⁷, Sonja Schrepfer^1,2,7

¹University Heart Center Hamburg, TSI-Lab, Germany, ²Cardiovascular Research Center, University of Hamburg, ³Department of Medicine, Cardiology Division, Pulmonary Hypertension Program, University of Alberta, ⁴Department of Medicine, Stanford University School of Medicine , ⁵Department of Biomedical Sciences, Institute of Physiology, Pathophysiology, and Biophysics, University of Veterinary Medicine, Vienna, ⁶Translumina GmbH, Hechingen, ⁷Department of Cardiothoracic Surgery, Stanford University School of Medicine

This video shows a model to study the development of intimal hyperplasia after stent deployment using a human vessel (IMA) in an immunodeficient rat model.

Biology

Preparation of Pancreatic Acinar Cells for the Purpose of Calcium Imaging, Cell Injury Measurements, and Adenoviral Infection

Abrahim I. Orabi¹, Kamaldeen A. Muili¹, Dong Wang¹, Shunqian Jin¹, George Perides², Sohail Z. Husain¹

¹Rangos Research Center, Pediatric Gastroenterology, Hepatology, and Nutrition, Children's Hospital of Pittsburgh of UPMC, ²Department of Surgery, Tufts University Medical Center

We describe a reproducible method of preparing mouse pancreatic acinar cells from a mouse for the purpose of examining acinar cell calcium signals and cellular injury with physiologically and pathologically relevant stimuli. A method for adenoviral infection of these cells is also provided.

Medicine

Inducing Myointimal Hyperplasia Versus Atherosclerosis in Mice: An Introduction of Two Valid Models

Mandy Stubbendorff^*1,2, Xiaoqin Hua^*1,2, Tobias Deuse^1,2,3, Ziad Ali^4,5, Hermann Reichenspurner^2,3, Lars Maegdefessel⁶, Robert C. Robbins⁷, Sonja Schrepfer^1,2,3,4

¹Transplant and Stem Cell Immunobiology Lab, Cardiovascular Research Center, University Hospital Hamburg, ²Cardiovascular Research Center (CVRC) and DZHK University Hamburg, ³Department of Cardiovascular Surgery, University Heart Center Hamburg, ⁴Center for Interventional Vascular Therapy, Division of Cardiology, Columbia University, ⁵Cardiovascular Research Foundation, New York, ⁶Karolinska Institute, Stockholm, ⁷Department of Cardiothoracic Surgery, Stanford University School of Medicine, Falk Cardiovascular Research Center

This video shows two models of intimal plaque development in murine arteries and emphasizes the differences in myointimal hyperplasia and atherosclerosis.

Medicine

Catheterization of the Carotid Artery and Jugular Vein to Perform Hemodynamic Measures, Infusions and Blood Sampling in a Conscious Rat Model

Jing Feng¹, Yvonne Fitz¹, Yan Li¹, Melinda Fernandez¹, Irene Cortes Puch¹, Dong Wang¹, Stephanie Pazniokas², Brandon Bucher³, Xizhong Cui¹, Steven B. Solomon¹

¹Critical Care Medicine Department, Clinical Center, National Institutes of Health, ²Harvard Apparatus, ³ADInstruments

Vascular accesses to measure hemodynamics, provide fluids and perform blood sampling are important to any small animal model study. We present a technique for implanting catheters into the carotid artery and the common jugular vein in an anesthetized rat for connecting to a system to perform monitoring, infusions and sampling.

Medicine

Vein Interposition Model: A Suitable Model to Study Bypass Graft Patency

Dong Wang^1,2,3,4, Grigol Tediashvili^1,2,3, Simon Pecha⁴, Hermann Reichenspurner⁴, Tobias Deuse^1,2,3,4, Sonja Schrepfer^1,2,3,4

¹Transplant and Stem Cell Immunobiology Lab, University Heart Center Hamburg, ²Department of Surgery, Transplant and Stem Cell Immunobiology Lab, University of California San Francisco (UCSF), ³Cardiovascular Research Center (CVRC) and DZHK German Center for Cardiovascular Research), partner site Hamburg/Kiel/Luebeck, ⁴Cardiovascular Surgery, University Heart Center Hamburg

This video demonstrates a model to study the development of myointimal hyperplasia after venous interposition surgery in rats.

Medicine

Balloon-based Injury to Induce Myointimal Hyperplasia in the Mouse Abdominal Aorta

Grigol Tediashvili^1,2,3, Dong Wang^1,2,3,4, Hermann Reichenspurner⁴, Tobias Deuse^1,2,3,4, Sonja Schrepfer^1,2,3,4

¹Transplant and Stem Cell Immunobiology Lab, University Heart Center, ²Department of Surgery, Transplant and Stem Cell Immunobiology Lab, University of California San Francisco (UCSF), ³Cardiovascular Research Center (CVRC) and DZHK German Center for Cardiovascular Research, ⁴Cardiovascular Surgery, University Heart Center

This article demonstrates a murine model to study the development of myointimal hyperplasia (MH) after aortic balloon injury.

Medicine

Implantation of hiPSC-derived Cardiac-muscle Patches after Myocardial Injury in a Guinea Pig Model

Liesa Castro^1,2, Birgit Geertz³, Marina Reinsch^2,3, Bülent Aksehirlioglu³, Arne Hansen^2,3, Thomas Eschenhagen^2,3, Hermann Reichenspurner^1,2, Florian Weinberger^*2,3, Simon Pecha^*1,2

¹Department of Cardiovascular Surgery, University Heart Center Hamburg, ²partner site Hamburg/Kiel/Lübeck, German Centre for Cardiovascular Research (DZHK), ³Department of Experimental Pharmacology and Toxicology, Cardiovascular ResearchCenter, University Medical Center Hamburg-Eppendorf

Here we present a protocol for the induction of left ventricular cryoinjury followed by the implantation of a cardiac muscle patch, derived from human iPS-cell cardiomyocytes in a guinea pig model.

Medicine

A Cryoinjury Model to Study Myocardial Infarction in the Mouse

Dong Wang^*1,2, Grigol Tediashvili^*1,2, Xiaomeng Hu^1,2, Alessia Gravina², Sivan G. Marcus^1,2, Hao Zhang⁴, Jeffrey E Olgin⁴, Tobias Deuse^1,2,5, Sonja Schrepfer^1,2,3,5

¹Transplant and Stem Cell Immunobiology Lab, University Heart Center, ²Department of Surgery, Transplant and Stem Cell Immunobiology Lab, University of California San Francisco, ³Cardiovascular Research Center (CVRC) and DZHK German Center for Cardiovascular Research, ⁴Division of Cardiology, Cardiovascular Research Institute, University of California San Francisco, ⁵Cardiovascular Surgery, University Heart Center

This article demonstrates a model to study cardiac remodeling after myocardial cryoinjury in mice.