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Journal - genetics

CRISPR Epigenome Editing in Human Cells using Plasmid DNA Transfection and mRNA Nucleofection Delivery

CRISPR Epigenome Editing in Human Cells using Plasmid DNA Transfection and mRNA Nucleofection Delivery

350 views

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2025

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Anna E. Christenson^1*, Nikita S. Divekar^1*, Mitzi G. Hernández Zamora¹, Carolyn D. Nguyen¹, James K. Nuñez^1,2

¹Department of Molecular and Cell Biology, University of California, Berkeley, ²Chan Zuckerberg Biohub - San Francisco

<em>In Vivo</em> Monitoring of Transcriptional Activity During Metabolic Transition Using a Bioluminescent Reporter in Yeast

In Vivo Monitoring of Transcriptional Activity During Metabolic Transition Using a Bioluminescent Reporter in Yeast

319 views

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2025

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Felipe Muñoz-Guzmán^1,2,3, Pablo Quintrel^1,2, José Benavides-Parra^1,2, Catalina Muñoz-Tapia^2,3,4, Luis F. Larrondo^2,4, Francisco A. Cubillos^1,2,5

¹Facultad de Química y Biología, Departamento de Biología, Universidad de Santiago de Chile, ²ANID-Millennium Science Initiative-Millennium Institute for Integrative Biology (iBio), ³Centro Científico y Tecnológico de Excelencia Ciencia & Vida, Fundación Ciencia & Vida, ⁴Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, ⁵ANID-Millennium Nucleus of Patagonian Limit of Life (LiLi)

Novel And Efficient Method for <em>Drosophila</em> Heart Fluorescence Staining with Cryosectioning

Novel And Efficient Method for Drosophila Heart Fluorescence Staining with Cryosectioning

311 views

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2025

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John Watson¹, Sachin Budhathoki¹, Girish C. Melkani^1,2

¹Department of Pathology, Division of Molecular and Cellular Pathology, Heersink School of Medicine, The University of Alabama at Birmingham, ²UAB Nathan Shock Center, The University of Alabama at Birmingham

Single Nucleotide Polymorphism-sensitive FISH Detection of Locus-specific Ribosomal RNA Transcription in <em>Drosophila melanogaster</em>

Single Nucleotide Polymorphism-sensitive FISH Detection of Locus-specific Ribosomal RNA Transcription in Drosophila melanogaster

494 views

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2025

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Jackson Chen¹, Jonathan O. Nelson¹

¹Department of Biochemistry and Cell Biology, Stony Brook University

Fluorescence Labeling to Visualize Low-Expressed Proteins in Zebrafish

Fluorescence Labeling to Visualize Low-Expressed Proteins in Zebrafish

510 views

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2025

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Xuepu Jin^1*, Binghuang Zhang^1*, Yu Sun^1*, Yahan Duan¹, Junchen Lu¹, Jiannan Liu², Jiahuai Han^1,3,4, Yingying Zhang¹

¹State Key Laboratory of Cellular Stress Biology, School of Life Sciences, Faculty of Medicine and Life Sciences, Xiamen University, ²Technology Center, Shanxi Xinghuacun Fenjiu Distillery Co., Ltd., ³Research Unit of Cellular Stress of CAMS, Cancer Research Center of Xiamen University, Xiang'an Hospital of Xiamen University, School of Medicine, Xiamen University, ⁴Laboratory Animal Center, Xiamen University

Genome Editing in the Yellow Fever Mosquito <em>Aedes aegypti</em> using CRISPR-Cas9

Genome Editing in the Yellow Fever Mosquito Aedes aegypti using CRISPR-Cas9

761 views

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2025

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Iliano V. Coutinho-Abreu^1*, Fangying Chen^1*, Hsing-Han Li^1*, Noah H. Rose², Omar S. Akbari¹

¹School of Biological Sciences, Section of Cell and Developmental Biology, University of California, San Diego, ²School of Biological Sciences, Department of Ecology, Behavior, and Evolution, University of California, San Diego

Identification of Functionally-Relevant Lentivirus Integration Sites in an Insertional Mutagenesis Cell Library

Identification of Functionally-Relevant Lentivirus Integration Sites in an Insertional Mutagenesis Cell Library

236 views

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2025

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Dongyang Xu¹, Lu Tang¹, Philipp Kapranov²

¹Institute of Genomics, School of Medicine, Huaqiao University, ²State Key Laboratory of Cellular Stress Biology, School of Life Sciences, Xiamen University

Introductory Analysis and Validation of CUT&RUN Sequencing Data

Introductory Analysis and Validation of CUT&RUN Sequencing Data

2.2K views

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2024

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Junwoo Lee¹, Biji Chatterjee^1,2, Nakyung Oh¹, Dhurjhoti Saha¹, Yue Lu¹, Blaine Bartholomew¹, Charles A. Ishak^1,3

¹Department of Epigenetics and Molecular Carcinogenesis, University of Texas MD Anderson Cancer Center, ²Department of Genomic Medicine, University of Texas MD Anderson Cancer Center, ³Department of Gynecologic Oncology and Reproductive Medicine, University of Texas MD Anderson Cancer Center

Application of DNA Barcoding to Identify Medicinal Plants

Application of DNA Barcoding to Identify Medicinal Plants

1.4K views

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2024

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Shuai Yang^1,2, Shilin Chen^1,2, Jing Mo², Junfei Liu², Qi Pan², Chi Song^2,3

¹School of Pharmacy, Guizhou University of Traditional Chinese Medicine, ²School of Pharmacy/School of Modern Chinese Medicine Industry, Chengdu University of Traditional Chinese Medicine, ³Institute of Herbgenomics, Chengdu University of Traditional Chinese Medicine

CIRCLE-Seq for Interrogation of Off-Target Gene Editing

CIRCLE-Seq for Interrogation of Off-Target Gene Editing

536 views

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2024

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Jeffrey Inen^1,2, Chann Makara Han^1,2, David M. Farrel³, Ganna Bilousova^1,2, Igor Kogut^1,2

¹Department of Dermatology, University of Colorado School of Medicine, Anschutz Medical Campus, ²Gates Institute, University of Colorado School of Medicine, Anschutz Medical Campus, ³Department of Biochemistry and Molecular Genetics, University of Colorado School of Medicine, Anschutz Medical Campus

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