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Primer Extension Capture: Targeted Sequence Retrieval from Heavily Degraded DNA Sources

19.8K Views

15:28 min

September 3rd, 2009

September 3rd, 2009

19,844 Views

0:00

Title

1:03

Introduction

1:39

Amplification of Primary DNA LIbrary by PCR

4:41

Primer Extension

8:13

Extension Product Capture

12:17

Capture Product Amplification

12:52

Representative Results: PEC Retrieval of Neanderthal Mitochondrial Genomes

14:45

Conclusion

We present a method of targeted ancient DNA sequence retrieval, which we used to reconstruct the complete mitochondrial genomes of five Neandertal individuals. Comparison of these sequences with present day humans suggests that Neandertals had a long term low effective population size.

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