Immobilization of Multi-biocatalysts in Alginate Beads for Cofactor Regeneration and Improved Reusability

17.0K Views

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09:27 min

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April 22nd, 2016

DOI :

10.3791/53944-v

April 22nd, 2016

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16,960 Views

Hui Gao¹, Eshita Khera², Jung-Kul Lee¹, Fei Wen²

¹Department of Chemical Engineering, Konkuk University, ²Department of Chemical Engineering, University of Michigan

Capitoli

0:05

Title

0:45

Whole-cell Biocatalysts Preparation

2:05

Biosynthesis of L-xylulose by Coupling E. coli_HjLAD and E. coli_SpNOx for Cofactor Regeneration

3:51

Immobilization of Recombinant Whole-cell Catalysts in Calcium Alginate Beads

6:25

Stability Assay of Immobilized Biocatalysts for L-xylulose Production

7:15

Results: Co-immobilization of Whole-cell Biocatalysts Improves Reusability

8:55

Conclusion

Trascrizione

The overall goal of this experimental procedure is to demonstrate the use of a coupled whole cell biocatalytic system immobilized in alginate beads for improved production yield and reusability. This is my subject, I have answered key questions in

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Riepilogo

Presented is the protocol for co-immobilizing whole-cell biocatalysts for cofactor regeneration and improved reusability, using the production of L-xylulose as an example. The cofactor regeneration is achieved by coupling two Escherichia coli strains expressing functionally complementary enzymes; the whole-cell biocatalyst immobilization is achieved by cell encapsulation in calcium alginate beads.

Esplora Altri Video

Keywords Immobilization

Multi biocatalysts

Alginate Beads

Cofactor Regeneration

Reusability

Whole Cell Biocatalytic System

Biocatalysts

Stability

Cofactor Regenerating

E Coli

SpNOx