The goal of this protocol is to use chemiluminescence to quantify nitric-oxide in biological samples by quantifying levels of nitrite and nitrate, using triiodide and vanadium-chloride reducing solutions. This protocol provides a powerful tool for
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Nitric oxide (NO) is an important signaling molecule in vascular homeostasis. NO production in vivo is too low for direct measurement. Chemiluminescence provides useful insight into NO cycle via measuring its precursors and oxidation products, nitrite and nitrate. Nitrite / nitrate determination in body tissues and fluids is explained.