Name: micro/nano-scale strain distribution measurement from sampling moiré fringes
Uploaded: 2017-05-23T15:00:00.000+00:00
Duration: 6 min 56 s
Description: Watch this Scientific Journal Video about Micro/Nano-scale Strain Distribution Measurement from Sampling MoirÃ© Fringes at JoVE.com

Questo lavoro è stato sostenuto da JSPS KAKENHI, i numeri di sovvenzione JP16K17988 e JP16K05996 e dal Programma di promozione strategica dell&#39;Innovazione Strategica Cross-Ministerial Unit D66, Misurazione Innovativa e Analisi per i Materiali Strutturali (SIP-IMASM), gestito dall&#39;ufficio del mobile. Gli autori sono anche grati al Dott. Satoshi Kishimoto e Kimiyoshi Naito a NIMS per il loro materiale CFRP.

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Nella tecnica descritta, un passo impegnativo è la realizzazione della griglia di micro / nano scala o di griglia (abbreviata come griglia) 26 se sul modello non esiste un pattern periodico. Il passo della griglia deve essere uniforme prima della deformazione perché è un parametro importante per la misura della deformazione. Se il materiale è un metallo, una litografia in metallo o una ceramica, la litografia a nano d&#39;impronta UV o riscaldamento (NIL) 27 , la litog...

Le misure di deformazione a scala micro / nano sono fondamentali per valutare le proprietà meccaniche, i comportamenti di instabilità, le sollecitazioni residue e gli eventi di creazione di materiali avanzati. Poiché le tecniche ottiche sono non-contatti, a campo pieno e non distruttivo, sono stati sviluppati diversi metodi ottici per la misura della deformazione negli ultimi decenni. Negli ultimi anni, le tecniche di misura della deformazione a scala micro / nano includono principalmente i metodi moiré 1 , 2 , 3 , 4 , l&#39;analisi di fase geometrica (GPA) 5 , 6 , la trasformazione di Fourier (FT), la correlazione digitale di immagini (DIC) e Interferometria elettronica a speckle (ESPI). Tra queste tecniche, GPA e FT non sono adatti per misure di deformazione complesse perché esistono più frequenze. Il metodo DIC è simMa impotente contro il rumore, perché il vettore di deformazione è speckle casuale. Infine, ESPI è fortemente sensibile alla vibrazione. Tra i metodi di moiré micro / nano-scala, i metodi più comunemente utilizzati sono attualmente i metodi di scansione del microscopio moiré come moiré 7 , 8 , 9 , scansione laser moiré 10 , 11 e microscopio a forza atomica moiré 12 , E alcuni metodi di moiré basati sul microscopio, come il metodo moiré digitale / sovrapposto 13 , 14 , 15 e il metodo di moltiplicazione / frazionamento moiré 16 , 17 . Il metodo di moiré di scansione ha molti vantaggi, come un ampio campo visivo, alto rendimentoL&#39;insensibilità al rumore casuale. Tuttavia, il metodo di moiré tradizionale di scansione è scomodo per le misurazioni di ceppo 2D perché è necessario ruotare la fase di campionamento o la direzione di scansione per 90 ° e scansionare due volte per generare le frange in moire in due direzioni 18 . La rotazione ei processi di scansione doppio introducono errori di rotazione e richiedono molto tempo, influenzando seriamente l&#39;accuratezza della misurazione del ceppo 2D, in particolare per il ceppo di taglio. Anche se la tecnica temporale di spostamento di fase 19 , 20 può migliorare l&#39;accuratezza della misurazione della deformazione, richiede tempo e un dispositivo di spostamento di fase speciale non adatto per test dinamici. Il metodo di moiré di campionamento 21 , 22 ha un&#39;alta precisione nelle misure di spostamento ed è ora utilizzato principalmente per misure di deflessione sui ponti quando le automobili pculo. Per estendere il metodo del moiré di campionamento a misure di tensione 2D a micro / nano scala, è stato recentemente sviluppato un metodo di moiré di moltiplicazione ricostituito 23 da due frange di moiré di campionamento a due pixel, in cui le misure sono due volte sensibili e l&#39;ampio campo visivo della Viene mantenuto il metodo di scansione del moiré. Inoltre, il metodo di moiré di campionamento spostamento di fase è sviluppato anche dai fringoni moiré a campionamento multi-pixel, consentendo misure di strain ad alta precisione. Questo protocollo introdurrà la procedura dettagliata di misurazione del ceppo e dovrebbe aiutare i ricercatori e gli ingegneri a imparare a misurare la deformazione, migliorando i processi di produzione dei materiali e dei prodotti.

<table><tbody><tr><td>Automatic Polishing Machine</td><td>Marumoto Struers K.K.</td><td>LaboPol-30, Labor Force-100</td><td></td></tr><tr><td>Carbon Fiber Reinforced Plastic</td><td>Mitsubishi Plastics, Inc.&amp;nbsp;</td><td>HYEJ16M95DHX1</td><td></td></tr><tr><td>Computer</td><td>DELL Japan</td><td>VOSTRO</td><td>Can be replaced with another computer with C++ programming language</td></tr><tr><td>Image Recording Software</td><td>Lasertec Corporation</td><td>LMEYE7</td><td>Installed in a laser scanning microscope</td></tr><tr><td>Ion Coater</td><td>Japan Electron Optics Laboratory Ltd.</td><td>JEC3000F</td><td></td></tr><tr><td>Laser Scanning Microscope</td><td>Lasertec Corporation</td><td>OPTELICS HYBRID</td><td></td></tr><tr><td>Nanoimprint Device</td><td>Japan Laser Corporation&amp;nbsp;</td><td>EUN-4200</td><td>Can be replaced with a electron beam lithography device or a focused ion beam milling device</td></tr><tr><td>Nanoimprint Mold</td><td>SCIVAX Corporation</td><td>3.0&amp;mu;m pitch</td><td>Customized</td></tr><tr><td>Nanoimprint Resist</td><td>Toyo Gosei Co., Ltd&amp;nbsp;</td><td>PAK01</td><td></td></tr><tr><td>Polishing Solution</td><td>Marumoto Struers K.K.</td><td>DP-Spray P 15&amp;mu;m, 1&amp;mu;m, 0.25&amp;mu;m</td><td>Use from coarse to fine</td></tr><tr><td>Pipet</td><td>AS ONE Corporation</td><td>10mL</td><td></td></tr><tr><td>Sand Paper</td><td>Marumoto Struers K.K.</td><td>SiC Foil #320, #800</td><td>Use from coarse to fine</td></tr><tr><td>Spin Coater</td><td>MIKASA Corporation</td><td>MS-A100</td><td></td></tr></tbody></table>

micro/nano-scale strain distribution measurement from sampling moir&#233; fringes

Questo lavoro descrive la procedura di misurazione ei principi di una tecnica di moiré di campionamento per le misurazioni di deformazione a scala micro / nano a campo pieno. La tecnica sviluppata può essere eseguita in due modi: utilizzando il metodo moiré di moltiplicazione ricostruito o il metodo moiré di campionamento spostamento di fase. Quando l&#39;intensità della griglia del campione è di circa 2 pixel, vengono creati frange di moiré di campionamento a 2 pixel per ricostruire un modello di moiré di moltiplicazione per una misura di deformazione. Entrambe le sensibilità di spostamento e di tendenza sono due volte più elevate rispetto al metodo di moiré tradizionale di scansione nello stesso ampio campo visivo. Quando l&#39;intensità della griglia del campione è di circa o maggiore di 3 pixel, vengono creati fringere di moiré di campionamento a più pixel e una combinazione di tecniche di spostamento di fase per una misura di deformazione a campo pieno. L&#39;accuratezza della misurazione del ceppo è notevolmente migliorata e la misura automatica del batch è facilmente raggiungibile.Entrambi i metodi possono misurare le distribuzioni bidimensionali (2D) di una singola immagine di griglia senza ruotare il campione o le linee di scansione, come nelle tecniche moiré tradizionali. Come esempi, le distribuzioni di spostamento e deformazione 2D, inclusi i ceppi di taglio di due esemplari di plastica rinforzati con fibra di carbonio, sono stati misurati in prove di flessione a tre punti. Si prevede che la tecnica proposta gioca un ruolo importante nelle valutazioni quantitative non distruttive delle proprietà meccaniche, fenomeni di crepe e sollecitazioni residue di una varietà di materiali.

Le distribuzioni di spostamento e deformazione 2D di due campioni in plastica rinforzata con fibre di carbonio (CFRP) (n. 1 e n. 2) sono state misurate in base al principio di formazione del moiré 23 e al processo di misurazione ( Figura 1 ). I campioni CFRP sono stati costituiti da fibre di carbonio K13D di diametro 10-11 μm e resine epossidiche. La deformazione del CFRP # 1 è stata determinata utilizzando il metodo moiré di molt...

Watch this Scientific Journal Video about Micro/Nano-scale Strain Distribution Measurement from Sampling MoirÃ© Fringes at JoVE.com

Micro/Nano-scale Strain Distribution Measurement from Sampling Moir&#233; Fringes

Qui viene presentata una tecnica di moiré di campionamento con metodi di campionamento a pixel da 2 pixel e multispagine per misure di distribuzione di strain ad alta precisione a micro / nano scala.

Misura di distribuzione di sforzo micro / nano-scala da campionature Moiré Fringes

This work describes the measurement procedure and principles of a sampling moir&#233; technique for full-field micro/nano-scale deformation measurements. ...

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12.2K Views.  National Institute of Advanced Industrial Science and Technology (AIST).  Qui viene presentata una tecnica di moiré di campionamento con metodi di campionamento a pixel da 2 pixel e multispagine per misure di distribuzione di strain ad alta precisione a micro / nano scala. 

Video: Micro/Nano-scale Strain Distribution Measurement from Sampling Moiré Fringes

Measuring Material Microstructure Under Flow Using 1-2 Plane Flow-Small Angle Neutron Scattering

A new small-angle neutron scattering (SANS) sample environment optimized for studying the microstructure of complex fluids under simple shear flow is presented. The SANS shear cell consists of a concentric cylinder Couette geometry that is sealed and rotating about a horizontal axis so that the vorticity direction of the flow field is aligned with the neutron beam enabling scattering from the 1-2 plane of shear (velocity-velocity gradient, respectively). This approach is an advance over previous shear cell sample environments as there is a strong coupling between the bulk rheology and microstructural features in the 1-2 plane of shear. Flow-instabilities, such as shear banding, can also be studied by spatially resolved measurements. This is accomplished in this sample environment by using a narrow aperture for the neutron beam and scanning along the velocity gradient direction. Time resolved experiments, such as flow start-ups and large amplitude oscillatory shear flow are also possible by synchronization of the shear motion and time-resolved detection of scattered neutrons. Representative results using the methods outlined here demonstrate the useful nature of spatial resolution for measuring the microstructure of a wormlike micelle solution that exhibits shear banding, a phenomenon that can only be investigated by resolving the structure along the velocity gradient direction. Finally, potential improvements to the current design are discussed along with suggestions for supplementary experiments as motivation for future experiments on a broad range of complex fluids in a variety of shear motions.

A shear cell is developed for small-angle neutron scattering measurements in the velocity-velocity gradient plane of shear and is used to characterize complex fluids. Spatially resolved measurements in the velocity gradient direction are possible for studying shear-banding materials. Applications include investigations of colloidal dispersions, polymer solutions, and self-assembled structures.

Misurare Materiale microstruttura Under flusso Utilizzo di 1-2 aereo Flow-Small Angle Neutron Scattering

A new small-angle neutron scattering (SANS) sample environment optimized for studying the microstructure of complex fluids under simple shear flow is ...

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Implementation of a Reference Interferometer for Nanodetection

A thermally and mechanically stabilized fiber interferometer suited for examining ultra-high quality factor microcavities is fashioned. After assessing its free spectral range (FSR), the module is put in parallel with a fiber taper-microcavity system and then calibrated through isolating and eliminating random shifts in the laser frequency (i.e. laser jitter noise). To realize the taper-microcavity junction and to maximize the optical power that is transferred to the resonator, a single-mode optical fiber waveguide is pulled. Solutions containing polystyrene nanobeads are then prepared and flown to the microcavity in order to demonstrate the system&#8217;s ability to sense binding to the surface of the microcavity. Data is post-processed via adaptive curve fitting, which allows for high-resolution measurements of the quality factor as well as the plotting of time-dependent parameters, such as resonant wavelength and split frequency shifts. By carefully inspecting steps in the time-domain response and shifting in the frequency-domain response, this instrument can quantify discrete binding events.

A reference interferometer technique, which is designed to remove undesirable laser jitter noise for nanodetection, is utilized for probing an ultra-high quality factor microcavity. Instructions for assembly, setup, and data acquisition are provided, alongside the measurement process for specifying the cavity quality factor.

Realizzazione di un interferometro di riferimento per Nanodetection

A thermally and mechanically stabilized fiber interferometer suited for examining ultra-high quality factor microcavities is fashioned. After assessing ...

Stress Distribution During Cold Compression of Rocks and Mineral Aggregates Using Synchrotron-based X-Ray Diffraction

We report detailed procedures for performing compression experiments on rocks and mineral aggregates within a multi-anvil deformation apparatus (D-DIA) coupled with synchrotron X-radiation. A cube-shaped sample assembly is prepared and compressed, at room temperature, by a set of four X-ray transparent sintered diamond anvils and two tungsten carbide anvils, in the lateral and the vertical planes, respectively. All six anvils are housed within a 250-ton hydraulic press and driven inward simultaneously by two wedged guide blocks. A horizontal energy dispersive X-ray beam is projected through and diffracted by the sample assembly. The beam is commonly in the mode of either white or monochromatic X-ray. In the case of white X-ray, the diffracted X-rays are detected by a solid-state detector array that collects the resulting energy dispersive diffraction pattern. In the case of monochromatic X-ray, the diffracted pattern is recorded using a two-dimensional (2-D) detector, such as an imaging plate or a charge-coupled device (CCD) detector. The 2-D diffraction patterns are analyzed to derive lattice spacings. The elastic strains of the sample are derived from the atomic lattice spacing within grains. The stress is then calculated using the predetermined elastic modulus and the elastic strain. Furthermore, the stress distribution in two-dimensions allow for understanding how stress is distributed in different orientations. In addition, a scintillator in the X-ray path yields a visible light image of the sample environment, which allows for the precise measurement of sample length changes during the experiment, yielding a direct measurement of volume strain on the sample. This type of experiment can quantify the stress distribution within geomaterials, which can ultimately shed light on the mechanism responsible for compaction. Such knowledge has the potential to significantly improve our understanding of key processes in rock mechanics, geotechnical engineering, mineral physics, and material science applications where compactive processes are important.

We report detailed procedures for compression experiments on rocks and mineral aggregates within a multi-anvil deformation apparatus coupled with synchrotron X-radiation. Such experiments allow quantification of the stress distribution within samples, that ultimately sheds light on compaction processes in geomaterials.

Distribuzione delle sollecitazioni durante la compressione a freddo di rocce e inerti minerali mediante diffrazione di raggi x di sincrotrone-basato

We report detailed procedures for performing compression experiments on rocks and mineral aggregates within a multi-anvil deformation apparatus (D-DIA) ...

Ambiente

Observing and Quantifying Fibroblast-mediated Fibrin Gel Compaction

Cells embedded in collagen and fibrin gels attach and exert traction forces on the fibers of the gel. These forces can lead to local and global reorganization and realignment of the gel microstructure. This process proceeds in a complex manner that is dependent in part on the interplay between the location of the cells, the geometry of the gel, and the mechanical constraints on the gel. To better understand how these variables produce global fiber alignment patterns, we use time-lapse differential interference contrast (DIC) microscopy coupled with an environmentally controlled bioreactor to observe the compaction process between geometrically spaced explants (clusters of fibroblasts). The images are then analyzed with a custom image processing algorithm to obtain maps of the strain. The information obtained from this technique can be used to probe the mechanobiology of various cell-matrix interactions, which has important implications for understanding processes in wound healing, disease development, and tissue engineering applications.

Time-lapse microscopy and image processing techniques were used to observe and analyze fibroblast-mediated gel compaction and fibrin fiber realignment in an environmentally controlled bioreactor over a 48 hr&#160;period.

Osservazione e quantificazione della compattazione del gel di fibrina mediata da fibroblasti

Cells embedded in collagen and fibrin gels attach and exert traction forces on the fibers of the gel. These forces can lead to local and global ...

Bioingegneria

Synchrotron X-ray Microdiffraction and Fluorescence Imaging of Mineral and Rock Samples

In this report, we describe a detailed procedure for acquiring and processing&#160;x-ray microfluorescence (&#956;XRF), and Laue and powder microdiffraction two-dimensional (2D) maps at beamline 12.3.2 of the Advanced Light Source (ALS), Lawrence Berkeley National Laboratory. Measurements can be performed on any sample that is less than 10 cm x 10 cm x 5 cm, with a flat exposed surface. The experimental geometry is calibrated using standard materials (elemental standards for XRF, and crystalline samples such as Si, quartz, or Al2O3 for diffraction). Samples are aligned to the focal point of the x-ray microbeam, and raster scans are performed, where each pixel of a map corresponds to one measurement, e.g., one XRF spectrum or one diffraction pattern. The data are then processed using the in-house developed software XMAS, which outputs text files, where each row corresponds to a pixel position. Representative data from moissanite and an olive snail shell are presented to demonstrate data quality, collection, and analysis strategies.

We describe a beamline setup meant to carry out rapid two-dimensional x-ray fluorescence and x-ray microdiffraction mapping of single crystal or powder samples using either Laue (polychromatic radiation) or powder (monochromatic radiation) diffraction. The resulting maps give information about strain, orientation, phase distribution, and plastic deformation.

Microdiffrazione dei raggi x di sincrotrone e formazione immagine di fluorescenza del minerale e campioni di roccia

In this report, we describe a detailed procedure for acquiring and processing&#160;x-ray microfluorescence (&#956;XRF), and Laue and powder ...

Full-field Strain Measurements for Microstructurally Small Fatigue Crack Propagation Using Digital Image Correlation Method

A novel measurement approach is used to reveal the cumulative deformation field at a sub-grain level and to study the influence of microstructure on the growth of microstructurally small fatigue cracks. The proposed strain field analysis methodology is based on the use of a unique pattering technique with a characteristic speckle size of approximately 10 &#181;m. The developed methodology is applied to study the small fatigue crack behavior in body centered cubic (bcc) Fe-Cr ferritic stainless steel with a relatively large grain size allowing a high spatial measurement accuracy at the sub-grain level. This methodology allows the measurement of small fatigue crack growth retardation events and associated intermittent shear strain localization zones ahead of the crack tip. In addition, this can be correlated with the grain orientation and size. Thus, the developed methodology can provide a deeper fundamental understanding of the small fatigue crack growth behavior, required for the development of robust theoretical models for the small fatigue crack&#160;propagation in polycrystalline materials.

Microstructurally small fatigue crack growth behavior is investigated using a novel methodological approach combining crack growth rate measurement and strain-field analysis to reveal the cumulative deformation field at sub-grain level.

Misure estensimetriche a pieno campo per affaticamento Microstructurally piccola crepa propagazione utilizzando il metodo di correlazione di immagini digitali

A novel measurement approach is used to reveal the cumulative deformation field at a sub-grain level and to study the influence of microstructure on the ...

Spectral and Angle-Resolved Magneto-Optical Characterization of Photonic Nanostructures

Photonic crystals are periodic nanostructures that can support a variety of confined electromagnetic modes. Such confined modes are usually accompanied by local enhancement of electric field intensity that strengthens light-matter interactions, enabling applications such as surface-enhanced Raman scattering (SERS) and surface plasmon enhanced sensing. In the presence of magneto-optically active materials, the local field enhancement gives rise to anomalous magneto-optical activity. Typically, the confined modes of a given photonic crystal depend strongly on the wavelength and incidence angle of the incident electromagnetic radiation. Thus, spectral and angular-resolved measurements are needed to fully identify them as well as to establish their relationship with the magneto-optical activity of the crystal. In this article, we describe how to use a Fourier-plane (back focal plane) microscope to characterize magneto-optically active samples. As a model system, here we use a plasmonic grating built out of magneto-optically active Au/Co/Au multilayer. In the experiments, we apply a magnetic field on the grating in situ and measure its reciprocal space response, obtaining the magneto-optical response of the grating over a range of wavelengths and incident angles. This information enables us to build a complete map of the plasmonic band structure of the grating and the angle and wavelength dependent magneto-optical activity. These two images allow us to pinpoint the effect that the plasmon resonances have on the magneto-optical response of the grating. The relatively small magnitude of magneto-optical effects requires a careful treatment of the acquired optical signals. To this end, an image processing protocol for obtaining magneto-optical response from the acquired raw data is laid out.

Photonic band structure enables understanding how confined electromagnetic modes propagate within a photonic crystal. In photonic crystals that incorporate magnetic elements, such confined and resonant optical modes are accompanied by enhanced and modified magneto-optical activity. We describe a measurement procedure to extract the magneto-optical band structure by Fourier space microscopy.

Caratterizzazione magneto-ottica spectrale e sistemata con angolo di nanostrutture fotoniche

Photonic crystals are periodic nanostructures that can support a variety of confined electromagnetic modes. Such confined modes are usually accompanied by ...

Production of a Strain-Measuring Device with an Improved 3D Printer

A traditional strain measurement sensor needs to be electrified and is susceptible to electromagnetic interference. In order to solve the fluctuations in the analog electrical signal in a traditional strain gauge operation, a new strain measurement method is presented here. It uses a photographic technique to display the strain change by amplifying the change of the pointer displacement of the mechanism. A visual polydimethylsiloxane (PDMS) lens with a focal length of 7.16 mm was added to a smartphone camera to generate a lens group acting as a microscope to capture images. It had an equivalent focal length of 5.74 mm. Acrylonitrile butadiene styrene (ABS) and nylon amplifiers were used to test the influence of different materials on the sensor performance. The production of the amplifiers and PDMS lens is based on improved 3D printing technology. The data obtained were compared with the results from finite element analysis (FEA) to verify their validity. The sensitivity of the ABS amplifier was 36.03 &plusmn; 1.34 &#181;&epsilon;/&#181;m, and the sensitivity of the nylon amplifier was 36.55 &plusmn; 0.53 &#181;&epsilon;/&#181;m.

This work presents a strain measurement sensor consisting of an amplification mechanism and a polydimethylsiloxane microscope manufactured using an improved 3D printer.

Produzione di un dispositivo di misurazione della deformazione con una stampante 3D migliorata

A traditional strain measurement sensor needs to be electrified and is susceptible to electromagnetic interference. In order to solve the fluctuations in ...

Microtensiometer for Confocal Microscopy Visualization of Dynamic Interfaces

Adsorption of surface-active molecules to fluid-fluid interfaces is ubiquitous in nature. Characterizing these interfaces requires measuring surfactant adsorption rates, evaluating equilibrium surface tensions as a function of bulk surfactant concentration, and relating how surface tension changes with changes in the interfacial area following equilibration. Simultaneous visualization of the interface using fluorescence imaging with a high-speed confocal microscope allows the direct evaluation of structure-function relationships. In the capillary pressure microtensiometer (CPM), a hemispherical air bubble is pinned at the end of the capillary in a 1 mL volume liquid reservoir. The capillary pressure across the bubble interface is controlled via a commercial microfluidic flow controller that allows for model-based pressure, bubble curvature, or bubble area control based on the Laplace equation. Compared to previous techniques such as the Langmuir trough and pendant drop, the measurement and control precision and response time are greatly enhanced; capillary pressure variations can be applied and controlled in milliseconds. The dynamic response of the bubble interface is visualized via a second optical lens as the bubble expands and contracts. The bubble contour is fit to a circular profile to determine the bubble curvature radius, R, as well as any deviations from circularity that would invalidate the results. The Laplace equation is used to determine the dynamic surface tension of the interface. Following equilibration, small pressure oscillations can be imposed by the computer-controlled microfluidic pump to oscillate the bubble radius (frequencies of 0.001-100 cycles/min) to determine the dilatational modulus The overall dimensions of the system are sufficiently small that the microtensiometer fits under the lens of a high-speed confocal microscope allowing fluorescently tagged chemical species to be quantitatively tracked with submicron lateral resolution.

This manuscript describes the design and operation of a microtensiometer/confocal microscope to do simultaneous measurements of interfacial tension and surface dilatational rheology while visualizing the interfacial morphology. This provides the real-time construction of structure-property relationships of interfaces important in technology and physiology.

Microtensiometro per microscopia confocale Visualizzazione di interfacce dinamiche

Adsorption of surface-active molecules to fluid-fluid interfaces is ubiquitous in nature. Characterizing these interfaces requires measuring surfactant ...

Controlled Strain of 3D Hydrogels under Live Microscopy Imaging

External forces are an important factor in tissue formation, development, and maintenance. The effects of these forces are often studied using specialized in vitro stretching methods. Various available systems use 2D substrate-based stretchers, while the accessibility of 3D techniques to strain soft hydrogels, is more restricted. Here, we describe a method that allows external stretching of soft hydrogels from their circumference, using an elastic silicone strip as the sample carrier. The stretching system utilized in this protocol is constructed from 3D-printed parts and low-cost electronics, making it simple and easy to replicate in other labs. The experimental process begins with polymerizing thick (&#62;100 &#956;m) soft fibrin hydrogels (Elastic Modulus of ~100 Pa) in a cut-out at the center of a silicone strip. Silicone-gel constructs are then attached to the printed-stretching device and placed on the confocal microscope stage. Under live microscopy the stretching device is activated, and the gels are imaged at various stretch magnitudes. Image processing is then used to quantify the resulting gel deformations, demonstrating relatively homogenous strains and fiber alignment throughout the gel&#8217;s 3D thickness (Z-axis). Advantages of this method include the ability to strain extremely soft hydrogels in 3D while executing in situ microscopy, and the freedom to manipulate the geometry and size of the sample according to the user&#8217;s needs. Additionally, with proper adaptation, this method can be used to stretch other types of hydrogels (e.g., collagen, polyacrylamide or polyethylene glycol) and can allow for analysis of cells and tissue response to external forces under more biomimetic 3D conditions.

The presented method involves uniaxial stretching of 3D soft hydrogels embedded in silicone rubber while allowing live confocal microscopy. Characterization of the external and internal hydrogel strains as well as fiber alignment are demonstrated. The device and protocol developed can assess the response of cells to various strain regimes.

Ceppo controllato di idrogel 3D in imaging a microscopia dal vivo

External forces are an important factor in tissue formation, development, and maintenance. The effects of these forces are often studied using specialized ...