Analisi completa della metilazione del DNA usando un metodo basato sulla cattura di dominio di metile-CpG in pazienti con leucemia linfocitica cronica

11:26

Conclusion

10:09

Results: Identification of Differentially Methylated CpG Rich Regions in Chronic Lymphocytic Leukemia (CLL) Samples

0:49

Genomic DNA Extraction and Sonication

2:06

Bead Preparation Prior to Binding with Methyl-CpG-binding Domain (MBD)-biotin Protein

3:35

Binding the MBD-biotin Protein to the Washed Beads

6:22

Removing the Unbound DNA and Eluting the Methylated DNA from the Beads

8:10

Ethanol Precipitation and the Enrichment of Methylated DNA

4:59

Binding MBD-biotin Beads with Fragmented Genomic DNA