Preparation of Cryosections from Electroporated Cerebella
6:22
Immunostaining the Cerebellar Cryosections
7:38
Results: Immunostained Cerebella from Rosa26-CAG-LSL-Cas9-P2A-EGFP Mouse
8:48
Conclusion
Trascrizione
The overall goal of this electroporation-based in vivo gene transfer approach is to evaluate the roles of genes of interest in differentiation of cerebellar granule cells using CRISPR-mediated loss of function analyses. This method can help answer
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Conventional loss-of-function studies of genes using knockout animals have often been costly and time-consuming. Electroporation-based CRISPR-mediated somatic mutagenesis is a powerful tool to understand gene functions in vivo. Here, we report a method to analyze knockout phenotypes in proliferating cells of the cerebellum.