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Light-sheet Microscopy for Three-dimensional Visualization of Human Immune Cells

DOI :

10.3791/57651-v

June 13th, 2018

June 13th, 2018

7,910 Views

1Department of Biophysics, Center for Integrative Physiology and Molecular Medicine (CIPMM), School of Medicine, Saarland University

Here, we present a protocol to visualize immune cells embedded in a three-dimensional (3D) collagen matrix using light-sheet microscopy. This protocol also elaborates how to track cell migration in 3D. This protocol can be employed for other types of suspension cells in the 3D matrix.

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Light sheet Microscopy

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