Sample Preparation for Light-sheet Fluorescence Microscopy Using Capillaries
4:08
Image Acquisition Using Light-sheet Microscopy
5:07
Fixation and Immunofluorescence Staining of Cells in Collagen Matrices
7:17
Results: Visualization of Human CTL Cells by Light-sheet Microscopy
8:32
Conclusion
Transcription
This method can help answer key questions in the immunology field such as how exactly immune cells behave in a physiologically relevant context like how to search target cells efficiently in a three-dimensional environment. The main advantage of t
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Here, we present a protocol to visualize immune cells embedded in a three-dimensional (3D) collagen matrix using light-sheet microscopy. This protocol also elaborates how to track cell migration in 3D. This protocol can be employed for other types of suspension cells in the 3D matrix.