Pretreatment of Slides for Preparation of RNA Probe Hybridization
3:03
Hybridization of the RNA Probe
4:07
Visualization of the RNA Probe
6:18
Results: Adult Corals Exposed to Heat Stress Show Spatial Expression of RNA Probes
7:07
Conclusion
Trascrizione
This method can help answer key questions in coral cell biology, such as what types of tissues and cells express specific types of genes. The main advantage of this technique is that you have visual information on RNA expression. Though this metho
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The goal of this protocol is to perform in situ hybridization on adult coral samples that have been embedded in paraffin and sectioned onto glass slides. This is a qualitative method used to visualize the spatial expression of an RNA anti-sense probe in paraffin-embedded tissues.