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Renal Subcapsular Transplantation of 2'-Deoxyguanosine-Treated Murine Embryonic Thymus in Nude Mice

DOI :

10.3791/59657-v

•

July 19th, 2019

July 19th, 2019

•

10,118 Views

Jianxue Wang^1,3,4, Guolin Chen^1,3,4, Qianwen Cui^2,3,4, Erfei Song^1,3,4, Weihua Tao¹, Wanqun Chen², Cunchuan Wang^1,3,4, Shiqi Jia^1,3,4

¹The First Affiliated Hospital, Jinan University, ²Department of Biochemistry and Molecular Biology, School of Basic Medical Science, Jinan University, ³The Institute of Clinical Medicine, Jinan University, ⁴Joint institute of Metabolic Medicine Between Jinan University and University of Hong Kong

Chapters

We provide a simple and efficient method to transplant 2’-deoxyguanosine treated E18.5 thymus into the renal capsule of a nude mouse. This method should aide in the study of both thymic epithelial cells function and T cells maturation.

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