Improving the Accuracy of Flow Cytometric Assessment of Mitochondrial Membrane Potential in Hematopoietic Stem and Progenitor Cells Through the Inhibition of Efflux Pumps
Immunostaining for Detection of Hematopoietic Stem Cells
3:28
TMRM Staining
4:17
Flow Cytometry
5:38
Results: Flow Cytometry Analysis of Mitochondrial Membrane Potential
6:33
Conclusion
Trascrizione
Mitochondrial function is critical for hematopoietic stem cell self renewal. Mitochondrial activity is a reflector of the inner mitochondrial membrane potential, which can be measured by TMRM, a cell fluorescent, cell-permeant cationic dye. Xenobi
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Xenobiotic efflux pumps are highly active in hematopoietic stem and progenitor cells (HSPCs) and cause extrusion of TMRM, a mitochondrial membrane potential fluorescent dye. Here, we present a protocol to accurately measure mitochondrial membrane potential in HSPCs by TMRM in the presence of Verapamil, an efflux pump inhibitor.