Improving the Accuracy of Flow Cytometric Assessment of Mitochondrial Membrane Potential in Hematopoietic Stem and Progenitor Cells Through the Inhibition of Efflux Pumps

7.7K Views

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07:17 min

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July 30th, 2019

DOI :

10.3791/60057-v

July 30th, 2019

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Claudia Morganti¹^,²^,³, Massimo Bonora¹^,²^,³, Keisuke Ito¹^,²^,³^,⁴

¹Ruth L. and David S. Gottesman Institute for Stem Cell and Regenerative Medicine Research, Albert Einstein College of Medicine, ²Departments of Cell Biology and Stem Cell Institute, Albert Einstein College of Medicine, ³Department of Medicine, Albert Einstein College of Medicine, ⁴Albert Einstein Cancer Center and Diabetes Research Center, Albert Einstein College of Medicine

Summary

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Flow Cytometry

Mitochondrial Membrane Potential

Hematopoietic Stem Cells

TMRM

Efflux Pumps

Verapamil

Bone Marrow Isolation

Staining Buffer

Lineage Cocktail

Fluorophore Conjugated Antibodies

Mononuclear Cells

CD135 Biotinylated Antibodies

Chapters in this video

0:04

Title

0:43

Bone Marrow Isolation

2:22

Immunostaining for Detection of Hematopoietic Stem Cells

3:28

TMRM Staining

4:17

Flow Cytometry

5:38

Results: Flow Cytometry Analysis of Mitochondrial Membrane Potential

6:33

Conclusion

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