Defining Gene Functions in Tumorigenesis by Ex vivo Ablation of Floxed Alleles in Malignant Peripheral Nerve Sheath Tumor Cells

DOI :

10.3791/62740-v

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August 25th, 2021

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1,613 Views

Jody Fromm Longo¹, Stephanie N. Brosius^2,3, Steven L. Carroll¹

¹Department of Pathology and Laboratory Medicine, Medical University of South Carolina, ²Department of Pediatrics and Neurology, Perelman School of Medicine of the University of Pennsylvania, ³Division of Child Neurology, Children's Hospital of Philadelphia

Chapters

Here, we present a protocol for performing gene knockouts that are embryonic lethal in vivo in genetically engineered mouse model-derived tumors and then assessing the effect that the knockout has on tumor growth, proliferation, survival, migration, invasion, and the transcriptome in vitro and in vivo.

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