Seeding and Differentiation of Airway Epithelial Cells and Maintenance of Differentiated ALI Models
4:12
Three-Dimensional Airway Epithelial Organoids
5:54
Imaging Cilia Beat Frequency
7:18
Data Analysis and Quantification of Cilia Beat Frequency
9:20
Results: Cilia Beat Frequency in Airway Epithelial Cell ALI Models of Cystic Fibrosis Patients and Healthy Participants
10:36
Conclusion
Trascrizione
We describe the collection of airway stem cells from nasal mucosa. These cells are expanded and differentiated into a pseudostratified epithelium in the lab. This is in vitro expansion and differentiation, which is similar to our airways in vivo,
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This protocol describes nasal epithelial cell collection, expansion, and differentiation to organotypic airway epithelial cell models and quantification of cilia beat frequency via live-cell imaging and custom-built scripts.