Probing Mitochondrial Energy Metabolism of Single Spheroids Using XF Technology
2:44
Preparation of XF Assay Medium
3:08
Preparation of the Sensor Cartridge
4:25
Seeding Single Spheroids into the Spheroid Assay Microplate
5:04
Preparation and Loading of Compounds into the Sensor Cartridge for XF Assays
5:31
Results: Mitochondrial Energy Metabolism in 3D Cancer Cell-Line-Derived Spheroids
7:35
Conclusion
Trascrizione
Our protocol allows researchers to probe the mitochondrial energy metabolism and seamless spheroids to allow comparisons to be made between several different cell lines, cell numbers and phenotypes, as well as drug treatments in a of field drug di
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These protocols will help users probe mitochondrial energy metabolism in 3D cancer cell-line-derived spheroids using Seahorse extracellular flux analysis.