Harvesting and Pre-digestion of Hearts from Neonatal Mice
2:43
Enzymatic Digestion and Cell Plating
6:56
Oxygen Consumption Assay Using a 96-well Format Extracellular Flux Analyzer
9:03
Results: Mitochondrial Stress Assay
10:34
Conclusion
Trascrizione
This protocol allows us to isolate and culture high bi-BBT, known only to mouse cardiomyocytes. To evaluate mitochondrial function, oxygen consumption rate of cardiomyocytes can be analyzed by an actual silver flux analyzer. One of the advantages
Accedi o inizia la tua prova gratuita per vedere questo contenuto
The goal of this protocol is to illustrate how to use mouse neonatal cardiomyocytes as a model system to examine how various factors can alter oxygen consumption in the heart.