We would like to acknowledge the funding contributions of the Canadian Surgical Research Fund, the Edmonton Civic Employees Research Assistance Fund, and the Alberta IBD Consortium through a grant from Alberta Innovates.

動物使用プロトコルはアルバータ大学の健康科学動物管理使用委員会によって承認された。 楽器、動物および手術のセットアップの調製<ol><li>手続きの前に、すべての固形食24 hrの新しい、クリーンなケージ不在に動物を転送します。彼らは、手続きの時間になるまで水に自由にアクセスし、液体ダイエットアドリブを持っている場合があります。 </li><li>手続きに必要なすべての楽器をオートクレーブ。クリーン操作面、70％エタノールで麻酔ノーズコーン。 </li><li>執刀医のための快適な方法で手術用顕微鏡、麻酔器や電源で操作面を設定します。外科医の肘、手と用具を遮るもののない腕で、手術台の上で快適休ませなければならない。楽器、縫合糸、綿棒、および10ミリリットルの注射器は、手順の間に簡単にアクセスを許可した場所に配置する必要があります。 </li><li>セットアップオーバーヘッド加熱ランプは、操作面の手順と光の間の動物のための暖かさの両方を提供する。 </li><li> 0.9％食塩水で50ml円錐管、および操作面の近くにワセリンと場所との1.5mlチューブを埋める。 注：すべての楽器や外科用品を滅菌する。腸が離断されているので、手続き自体は無菌ではありません。これは、クリーンで汚染されたと考えられている。感染の外因性源の導入を回避するための措置をとる。 </li></ol>吻合2.回結腸切除術<ol><li>動物の呼吸数が約30〜40回/分に減速するまで、イソフルラン気化器からノーズコーンを介して、2リットル/分の酸素流量で4％イソフルランを投与することにより麻酔を誘導する。手順を開始する前に、痛みの応答がないことを確認するには、マウスの後肢足に適度な圧力を適用します。この時点で、0.5L /分に2％イソフルランと酸素の流れを下げてください。断続的に処置中に痛みの応答を確認し、それに応じて、イソフルラン流量を調節。 </li><li>手術中の乾燥を防止し、透明テープを用いて固定手足と仰臥位でマウスを固定化するために眼にワセリンを適用する。 </li><li> povodine /ヨウ素溶液で腹部をきれいにし、新しい滅菌手袋に着替える。 </li><li>筋膜と腹膜を露出するためにハサミを解剖鋭いポイントを使用して腹部の上部正中1.5センチの皮膚切開を行います。腹膜内容を公開する白線を通して同様の方法で筋膜/腹膜層を開きます。 </li><li>ヒトとは対照的に、マウスの盲腸は、典型的には、腹部の左上腹部に見られる。特定されたら、優しく鉗子で盲腸を把握し、切開を通してそれを実現します。腹部の表面にドレープ滅菌ガーゼ（ 図1A）上で盲腸から延びるファンアウトに回腸末端の約3cmを湿らせた綿棒を使用してください。暴露腸がプロシージャの全体の間に0.9％生理食塩水で湿った保たれていることを確認。 </li><li>手術用顕微鏡を用いて、コロン（ 図1A）に沿って上腸間膜動脈から分岐回盲動脈を識別します。回盲動脈に隣接する無血管組織を切開、5-0絹のネクタイで動脈を包囲し、連結する。次に、回腸末端に地域の血液供給を見つけ、回盲部に離断点1.5-2センチ近位の順に選択します。上記のように回腸のこのセクションに枝を結紮。はさみを解剖ミクロと動脈を分割します。 </li><li>離断終了する（ 図1B）に十分な血液供給があるの確保回腸および結腸の虚血性部分を分ける。それはより密接にコロンと一致するように内腔の直径を増加させるために30度の角度で分割して回腸をへらすることがしばしば有用である。腸の回盲部が除去されて、一度、整列各腸間膜の国境を確保ガーゼ上の回腸および結腸の離断端は、整列している。 </li><li> 8-0ポリプロピレン縫合糸（ 図1C）を使用して中断し、コロンの離断最後に回腸の離断終わりを近似することにより吻合を構築します。最初のステッチは、回結腸吻合水密になるまで、すべての0.5ミリメートルを置いその後の縫合糸で、腸間膜の国境に配置されます。回腸および結腸を通して縫合針を渡すとき、切り口がロールバックされていないことを確認し、針に刺されは、腸の切断端から0.5ミリメートルである。典型的な吻合は14から16に中断縫合が必要になります。吻合を通じて内容を強制的に回腸遠位の上、綿棒の近位を圧延することにより完了時に吻合の整合性と開存性をテストします。小腸の内容は自由に吻合漏れなく結腸に渡す必要があります。 </li><li> 10ミリリットルの注射器から0.9％食塩水3〜4 mlの暴露腸をすすぐ腸の表面から糞便を洗い流し、および腹腔内に戻って腸を提供します。 0.9％生理食塩水2 mlを用いて、腹膜腔を洗浄し、次いで横方向に腹壁に穏やかな圧力を適用することによって、この流体を排出する。 </li><li> 3-0絹ランニング縫合糸で切開を閉じ、イソフルランの流れを中止してください。術後疼痛管理のために-buprenorphine-皮下に長時間作用性アヘン剤の0.1 mg / kgをを管理します。 </li><li>彼らがモバイルになるまで継続的に温めてケージに転送した後、熱ランプの下で動物を観察します。 </li></ol> 3.術後ケアとモニタリング<ol><li>その日の残りのための苦痛の徴候のために継続的に温めケージに動物を監視します。背中流動食と水アドリブへのアクセス権を持つ新たな無菌ケージ内の動物飼育施設に動物を転送します。動物は3-4の群で収容することができる。 </li><li>翌朝術後動物のチェックを実行し、動物は苦痛には表示されないように。唯一の流動食を供給。彼らは不快に表示された場合（ すなわち、猫背の姿勢、または最小活性）皮下ブプレノルフィンの追加の用量を投与。術後1日目の午後に一回より多くの動物に確認してください。 </li><li>術後2日目の朝に動物が完全に回復し表示されるはずです。食料消費とstoolingの証拠は回復の明るい兆しである。さて、動物用固形飼料食を再開します。 注：苦痛の徴候は​​猫背の姿勢、貧しいグルーミング、および最小限のアクティビティが含まれます。苦痛の徴候が顕著である場合には、動物を安楽死させなければならない。 </li><li>動物が足圧に反応しないようになるまで2 L / minのO 2流量で4％イソフルランで深く麻酔を誘発することによって動物を安楽死させる。頸椎脱臼を行い、適切な安楽死の兆候を観察する。 </li><li>ガイドラインは異なりますので、適応症に関する機関の勧告を参照してください。用とマウスでの安楽死の適切な方法。 </li></ol>

<ol>
	<li>Hallet, J., Zih, F. S., Lemke, M., Milot, L., Smith, A. J., Wong, C. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Neo-adjuvant+chemoradiotherapy+and+multivisceral+resection+to+optimize+R0+resection+of+locally+recurrent+adherent+colon+cancer.">Neo-adjuvant chemoradiotherapy and multivisceral resection to optimize R0 resection of locally recurrent adherent colon cancer.</a> European Journal of Surgical Oncology. 40 (6), (2014).</li><li>Rutgeerts, P., Geboes, K., Vantrappen, G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Natural+history+of+recurrent+Crohn&#39;s+disease+at+the+ileocolonic+anastomosis+after+curative+surgery.">Natural history of recurrent Crohn&#39;s disease at the ileocolonic anastomosis after curative surgery.</a> Gut. 25 (6), 665-672 (1984).</li><li>Davis, D., Rivadeneira, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Complications+of+colorectal+anastomoses:+leaks,+strictures,+and+bleeding.">Complications of colorectal anastomoses: leaks, strictures, and bleeding.</a> The Surgical clinics of North America. 93 (1), 61-87 (2013).</li><li>Helmrath, M. A., VanderKolk, W. E., Can, G., Erwin, C. R., Warner, B. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Intestinal+adaptation+following+massive+small+bowel+resection+in+the+mouse.">Intestinal adaptation following massive small bowel resection in the mouse.</a> Journal of the American College of Surgeons. 183 (5), 441-449 (1996).</li><li>Dekaney, C. M., Fong, J. J., Rigby, R. J., Lund, P. K., Henning, S. J., Helmrath, M. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Expansion+of+intestinal+stem+cells+associated+with+long-term+adaptation+following+ileocecal+resection+in+mice.+American+journal+of+physiology.">Expansion of intestinal stem cells associated with long-term adaptation following ileocecal resection in mice. American journal of physiology.</a> Gastrointestinal and liver physiology. 293 (5), (2007).</li><li>Speck, K. E., De Cruz, P., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Inflammation+enhances+resection-induced+intestinal+adaptive+growth+in+IL-10+null+mice.">Inflammation enhances resection-induced intestinal adaptive growth in IL-10 null mice.</a> The Journal of surgical research. 168 (1), 62-69 (2011).</li><li>Rigby, R. J., Hunt, M. R., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+new+animal+model+of+postsurgical+bowel+inflammation+and+fibrosis:+the+effect+of+commensal+microflora.">A new animal model of postsurgical bowel inflammation and fibrosis: the effect of commensal microflora.</a> Gut. 58 (8), 1104-1112 (2009).</li><li>Kostic, A. D., Xavier, R. J., Gevers, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+Microbiome+in+Inflammatory+Bowel+Disease:+Current+Status+and+the+Future+Ahead.">The Microbiome in Inflammatory Bowel Disease: Current Status and the Future Ahead.</a> Gastroenterology. , 1-11 (2014).</li><li>Gevers, D., Kugathasan, S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+Treatment-Naive+Microbiome+in+New-Onset+Crohn&#39;s+Disease.">The Treatment-Naive Microbiome in New-Onset Crohn&#39;s Disease.</a> Cell host &amp; microbe. 15 (3), 382-392 (2014).</li><li>Ahmed, T., Rieder, F., Fiocchi, C., Achkar, J. P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Pathogenesis+of+postoperative+recurrence+in+Crohn&#39;s+disease.">Pathogenesis of postoperative recurrence in Crohn&#39;s disease.</a> Gut. 60 (4), 553-562 (2011).</li><li>Borowiec, A., Sydora, B., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Small+bowel+fibrosis+and+systemic+inflammatory+response+after+ileocolonic+anastomosis+in+IL-10+null+mice.">Small bowel fibrosis and systemic inflammatory response after ileocolonic anastomosis in IL-10 null mice.</a> Journal of Surgical Research. 178 (1), 147-154 (2012).</li><li>Devine, A. A., Gonzalez, A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> Impact of Ileocecal Resection and Concomitant Antibiotics on the Microbiome of the Murine Jejunum and Colon. 8 (8), (2013).</li><li>Bij, G. J., Oosterling, S. J., Beelen, R. H. J., Meijer, S., Coffey, J. C., van Egmond, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+perioperative+period+is+an+underutilized+window+of+therapeutic+opportunity+in+patients+with+colorectal+cancer.">The perioperative period is an underutilized window of therapeutic opportunity in patients with colorectal cancer.</a> Annals of surgery. 249 (5), (2009).</li><li>Scott, A. D., Uff, C., Phillips, R. K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Suppression+of+macrophage+function+by+suture+materials+and+anastomotic+recurrence+of+Crohn&#39;s+disease.">Suppression of macrophage function by suture materials and anastomotic recurrence of Crohn&#39;s disease.</a> The British journal of surgery. 80 (3), (1993).</li><li>Kiernan, J. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Intestinal+anastomosis+in+the+rat+facilitated+by+a+rapidly+digested+internal+splint+and+indigestible+but+absorbable+sutures.">Intestinal anastomosis in the rat facilitated by a rapidly digested internal splint and indigestible but absorbable sutures.</a> Journal of Surgical Research. 45, 427-431 (1988).</li><li>Andersen, T. L., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Action+of+matrix+metalloproteinases+at+restricted+sites+in+colon+anastomosis+repair:+an+immunohistochemical+and+biochemical+study.">Action of matrix metalloproteinases at restricted sites in colon anastomosis repair: an immunohistochemical and biochemical study.</a> Surgery. 140 (1), 72-82 (2006).</li><li>Thompson, S. K., Chang, E. Y., Jobe, B. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Clinical+review:+Healing+in+gastrointestinal+anastomoses,+Part+I.">Clinical review: Healing in gastrointestinal anastomoses, Part I.</a> Microsurgery. 26 (3), 131-136 (2006).</li></ol>

The authors have nothing to disclose

ICRマウスは、腸疾患における手術の効果を研究するために使用できる強力なモデルである。この記事では、95％の成功率と28日後の手順まで安定重みによって反映されるように成長障害と問題なくマウスではICRを行う方法を説明しています。成功したICRへの最も重大な課題は、吻合と吻合リークで腸閉塞を避けることが含まれる。 閉塞を防ぐための手術への技術要素は吻...

Ileocolic resection (ICR) is a common procedure performed in both emergent and elective situations for a variety of illnesses. Crohn&#8217;s disease and colon cancers are the two most common indications for ICR. In both illnesses, recurrence in the bowel at the site of surgery represents a major clinical problem. Local recurrence rates for colon cancer remain an issue even with the most aggressive resections1. Following ICR in Crohn&#8217;s disease, the disease most frequently (in up to 80%) recurs in the neo-terminal ileum at 1 year after surgery2. Given the impact of these two illnesses and their recurrence after surgery, it is important to understand local intestinal factors after ICR, which may have intrinsic influences on the natural history of these diseases. Further, it is also important to consider anastomotic healing after ICR. In the early post-operative period, anastomotic leaks can have devastating consequences for patients resulting in repeat surgeries, stoma creations, significant morbidity, and even mortality3. Despite the importance of this topic, our current understanding of anastomotic healing remains in its infancy as a subject of research. Animal models of ICR, in particular mice, are an excellent platform for studying the intestinal and anastomotic healing following surgery.
A mouse model of ICR was initially developed by Helmrath et al. to be used as a model of short gut syndrome4. The authors experimented with various diet regiments and suture sizes to optimize animal survival following ICR. They concluded that feeding with liquid diet in the perioperative period and using 9-0 monofilament sutures resulted in an optimal post-operative survival of 88%. Since this initial publication, ICR in mice removing 50% of the small bowel has been used in several studies to explore the dynamics of massive small bowel resection and the adaptive growth response in attempt to develop new therapies for short gut syndrome5,6.
The first application of the ICR mouse model to Crohn&#8217;s disease used the IL-10-/- mouse model, which spontaneously develops colitis7. The authors found that after ICR these animals developed inflammation in the neo-terminal ileum similar to that seen in post-operative Crohn&#8217;s disease patients, and that this inflammation was dependent on the presence of bacteria7. More recently, this model was used to explore bacterial changes induced by ICR. In Crohn&#8217;s disease there is an associated dysbiosis with relative decreases in bacteria known to have anti-inflammatory properties and increases in invasive species of bacteria8,9. The association holds true in cases of post-operative recurrence10. Two studies sought to identify microbial changes resulting from ICR. The first used IL-10 null mice, and performed denaturing gel electrophoresis to compare bacterial similarity between the small bowel and colon after ICR11. This study demonstrated that bacterial populations became similar in the small intestine and colon following ICR. A subsequent study used wild type mice and 16s pyrosequencing for phylogenetic classification of bacterial species post-operatively. This study demonstrated a marked shift in bacterial species resulting from surgery alone with Clostridium species becoming dominant as well as an increase in &#978;-proteobacteria. The results also confirmed the findings of the previous study with similar populations found in the small bowel and colon after ICR12. 
ICR is a common procedure for patients with colon cancer involving the cecum and ascending colon, and it is becoming increasingly recognized that the host-response to surgery likely contributes to both local and distant tumor recurrence13. Despite this observation, models of ICR have not been utilized for the study of colorectal cancer and post-operative recurrence. Understanding both the systemic and local immunologic changes resulting from ICR will be important in investigating future therapies. Potential pathways involved in cancer recurrence post ICR include up regulation of growth factors, which may rescue cells from apoptosis and stimulate proliferation, mechanical tumor disruption with cell shedding, and loss of immune surveillance through post-operative immunosuppression13,14. 
Mouse models of ICR have the potential to be a powerful tool for the investigation of short bowel syndrome, Crohn&#8217;s disease, and colon cancer. They may also provide lessons on how to prevent early post-operative anastomotic complications by further defining the cellular and biochemical pathways involved in healing the newly constructed anastomosis. A major barrier in utilizing the murine ICR model is the technical difficulty. The intestinal anastomosis requires the use of 8-0 or 9-0 suture, an operating microscope, and training in microsurgical techniques. The goal of this article is to provide clear instructions on how to perform ICR in mice with the goal of utilizing this procedure in models of disease.

<table border="0" cellpadding="0" cellspacing="0" width="665">
	<tbody>
		<tr height="42">
			<td height="42" style="height:42px;width:216px;">Name of Material/ Equipment</td>
			<td style="width:163px;">Company</td>
			<td style="width:119px;">Catalog Number</td>
			<td style="width:167px;">Comments/Description</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">LD101 liquid rodent diet</td>
			<td style="width:163px;">testdiet.com</td>
			<td style="width:119px;"></td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">0.9% NaCl</td>
			<td>Baxter</td>
			<td>FKE1324</td>
			<td>Injection quality saline</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Operating Microscope</td>
			<td>Ziess</td>
			<td></td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Isoflurane Anesthetic Vaporizer</td>
			<td>Harvard Apparatus</td>
			<td>34-0483</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Isoflurane</td>
			<td>Abbott&#160;</td>
			<td>05260-05</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Glass plate</td>
			<td></td>
			<td></td>
			<td>For operating surface</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Cotton swabs</td>
			<td></td>
			<td></td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Micro Castroviejo Needle holder, curved</td>
			<td>World Precision Instruments</td>
			<td align="right">503377</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Castroviejo straight scissors</td>
			<td>World Precision Instruments</td>
			<td>555530S</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Dissecting Scissors</td>
			<td>World Precision Instruments</td>
			<td align="right">15922</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Dressing Forceps x 2</td>
			<td>World Precision Instruments</td>
			<td align="right">500363</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">5-0 silk pre-cut sutures</td>
			<td>Ethicon</td>
			<td>A182H</td>
			<td>For vessel ligation</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">8-0 Prolene on BV130-5 needle</td>
			<td>Ethicon</td>
			<td>8732H</td>
			<td>For anastomosis</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">3-0 Silk on FS-2 needls</td>
			<td>Ethicon</td>
			<td>8665G</td>
			<td>For abdominal wall closure</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Petroleum Jelly</td>
			<td>Vaseline</td>
			<td></td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">10 ml syringe</td>
			<td>BD biosciences</td>
			<td></td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Povidone-iodine 7.5% surgical Scrub</td>
			<td><a href="http://betadine.com">betadine.com</a></td>
			<td></td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Heat lamps</td>
			<td></td>
			<td></td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">buprenorphine 0.3 mg/ml</td>
			<td>Reckitt Benckiser Healthcare Ltd.&#160;</td>
			<td>PL36699/0006</td>
			<td></td>
		</tr>
	</tbody>
</table>

murine ileocolic bowel resection with primary anastomosis

Intestinal resections are frequently required for treatment of diseases involving the gastrointestinal tract, with Crohn&#8217;s disease and colon cancer being two common examples. Despite the frequency of these procedures, a significant knowledge gap remains in describing the inherent effects of intestinal resection on host physiology and disease pathophysiology. This article provides detailed instructions for an ileocolic resection with primary end-to-end anastomosis in mice, as well as essential aspects of peri-operative care to maximize post-operative success. When followed closely, this procedure yields a 95% long-term survival rate, no failure to thrive, and minimizes post-operative complications of bowel obstruction and anastomotic leak. The technical challenges of performing the procedure in mice are a barrier to its wide spread use in research. The skills described in this article can be acquired without previous surgical experience. Once mastered, the murine ileocolic resection procedure will provide a reproducible tool for studying the effects of intestinal resection in models of human disease.

死亡率と体重変化術後。 129S1野生型マウスをICRに続く死亡率は〜5％、一般的である。道徳の最も一般的な原因は、吻合で腸閉塞である。死亡率の他の原因は、吻合部の漏れや閉塞を腸につながる内部ヘルニアが含まれています。 体重減少は術後14日まで見られるが、一般的に非有意であることができます。マウスが完全に術後28日目（ 図2）によ...

Watch this Scientific Journal Video about Murine Ileocolic Bowel Resection with Primary Anastomosis at JoVE.com

Murine Ileocolic Bowel Resection with Primary Anastomosis

Ileocolic resection is commonly performed in several human diseases; however, little is known regarding the impact of intestinal resection on surgical illnesses. This article provides instruction on executing the procedure in mice with high success, providing a means to study the effects of ileocolic resection in models of disease.

プライマリ吻合とネズミ回結腸腸切除

Intestinal resections are frequently required for treatment of diseases involving the gastrointestinal tract, with Crohn&#8217;s disease and colon cancer ...

murine-ileocolic-bowel-resection-with-primary-anastomosis

Research

JoVE Journal

Medicine

16.2K ビュー.  University of Alberta. Ileocolic resection is commonly performed in several human diseases; however, little is known regarding the impact of intestinal resection on surgical illnesses. This article provides instruction on executing the procedure in mice with high success, providing a means to study the effects of ileocolic resection in models of disease.