Name: whole vitreous humor dissection for vitreodynamic analysis
Uploaded: 2015-05-24T14:00:00.000+00:00
Duration: 4 min 41 s
Description: Watch this Scientific Journal Video about Whole Vitreous Humor Dissection for Vitreodynamic Analysis at JoVE.com

The authors acknowledge the following funding sources, Whittier Foundation, Harrington Foundation, National Institutes of Health and Research to Prevent Blindness.

すべて摘出した目は、食肉処理場から入手し、すべての実験は、機関のバイオセーフティ法に従って行いました。 <ol><li>表面に除核目を下に固定します。目の周りの過剰な組織を通じて組織ピンを配置し、発泡スチロールのボードにそれを固定することによってこれを行います。 </li><li>眼からperilimbal結膜を分析し、デタッチ。 <ol><li>輪部で結膜を切開し、ぶっきらぼう強膜からそれを分析するために微細な鉗子（0.3鉗子）とmicroscissors（ウェストコットのはさみ）を使用します。鈍的切開は、さらに切開を可能にするために進むように角膜輪部に沿って結膜をカット。 </li><li>できるだけ多くの強膜を露出させるためにすべての方法目（360度）の周りに結膜を削除します。 注：これは、残りの手順の間に外科的ピンやピンセットで目を固定容易にするために、結膜の少量を残して便利です。 </li></ol></li><li>一方に沿って十分な厚さの強膜フラップを作成目（手術用メスの刃69）の側面。 <ol><li>暗く着色された脈絡膜が表示されるまで、静かにメス（手術用メスの刃11）と強膜に切断して角膜縁に角膜​​輪部へ〜5ミリメ​​ートル強膜切開平行と3mmの後方を確認します。脈絡膜自体を切開しないように注意してください。 </li><li>それは内側に静かに脈絡膜を押すことにより、これらの組織との間の潜在的なスペースを大きくすることが可能になるまで慎重に、はさみ（鈍的切開を使用して）またはメスのいずれかで、強膜と脈絡膜の間に平面に沿って詳細に分析。 </li><li> T字型の切開を作成し、鋭いmicroscissorsとの最初の強膜切開に垂直な他の強膜切開を行います。 </li><li>そして、ぶっきらぼうに、基礎となる脈絡膜から強膜組織を除去し、穏やかに前述したように、強膜から離れて脈絡膜をプッシュする円周様式で解剖し続けています。 </li><li>必要に応じて、強膜弁を拡大。 <ol><li>静かに強膜から脈絡膜を解剖鈍プロセス全体。 </li><li>角膜輪部に垂直に切開が視神経に到達し、角膜輪部に切開製平行アイ円周（45°）の3分の1以上である。までフラップを拡大</li><li>鈍的切開中に目を操作するためのレバレッジのソースとして強膜弁を使用してください。フラップのカウンタートラクションを鈍的切開のためのそれが容易になります。 </li></ol></li><li>他の強膜弁の前の手順を繰り返します。 </li></ol></li><li>脈絡膜の大面積を露光するために強膜フラップを切り取ります。 </li><li>眼の周囲に、ステップ3で切開（360度）を続けます。 </li><li>残りの脈絡膜、網膜組織を削除します。 <ol><li>露出された領域内に、静かに残りの脈絡膜、網膜組織をはねのけるために綿の先端を使用しています。代わりに、静かに鉗子で組織を取得し、基本となる硝子体からそれを剥がします。 </li><li>必要に応じて、脈絡膜STに切開を作ります視神経からarting。その後、網膜と硝子体皮質を露出するために静かに脈絡膜を剥がします。 </li></ol></li><li>離れて強膜からの脈絡膜を解剖鈍を継続した後、全体を得るために、脈絡膜をはがし、そのまま硝子体。 </li><li>所望の位置に全体硝子体を配置するために角膜に付着した強膜リムを使用してください。この時点で、硝子体が前方強膜とレンズに取り付けられています。 </li><li>すべての強膜を除去し、レンズの周りに、強膜の内側から硝子体を解剖鈍いです。 </li><li>必要であれば離れた硝子体からのレンズをすくうために鈍いツールを使用します。様々なvitreodynamic実験用のサンプルを使用してください。 <ol><li>空気にさらされる既知の表面積を有するガラスビーカーに試料を配置します。サンプルの端に酸素感受性プローブ​​を配置します。サンプルに既知の距離（R）にプローブを移動させるためにマイクロマニピュレーターを使用してください。 </li><li>理論拡散方程式を得るためにフィックの法則を使用してください。ダとTAステップ10.1に収集等の実験拡散係数/反応項を得ます</li></ol></li></ol>

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H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Computer+modeling+of+drug+delivery+to+the+posterior+eye:+effect+of+active+transport+and+loss+to+choroidal+blood+flow.">Computer modeling of drug delivery to the posterior eye: effect of active transport and loss to choroidal blood flow.</a> Pharm Res. 25 (11), 2685-2696 (2008).</li><li>Smith, C. a., Newson, T. a., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+framework+for+modeling+ocular+drug+transport+and+flow+through+the+eye+using+micro-CT.">A framework for modeling ocular drug transport and flow through the eye using micro-CT.</a> Phys Med Biol. 57 (19), 6295-6307 (2012).</li><li>Quiram, P. A., Leverenz, V. R., Baker, R. M., Dang, L., Giblin, F. J., Trese, M. 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The authors have nothing to disclose.

慎重に硝子体解剖中に実行されなければならない2つの重要なステップがあります。全層強膜フラップを作成するステップ3は、全体の解剖に重要です。ケアは、全層強膜フラップを作成する際に脈絡膜にカットしないように注意する必要があります。他の重要なステップは、脈絡膜から強膜を離れて解剖されています。このステップは、慎重に硝子体がこぼれることができ、そこから脈絡膜?...

この方法の目的は、細部にvitreodynamic分析の目的のために、死体の眼から、硝子体コアと皮質無傷で、全体、無傷の硝子体を単離するための技術です。硝子体生理学の分野が成長してきたように、このような流体力学の研究者としての学際的研究者は、硝子体1の物理的および生体力学的特性を検討しています。そのためには、細部への学際的な研究者を支援するために、全体、無傷の硝子体を分離する技術が不可欠です。 セバーグら 2等3は、ヒト死体の目にエレガントな全体硝子体解剖を実施し、その結果の説明図を示しました。しかし、使用される技術は、詳細に記載されなかった非専門家が独立した方法を複製することはできません。他の研究は、このような吸引または部分的切開などの簡単な方法を使用して、死体眼から硝子体を収穫しましたこれらは両方とも全体、無傷の硝子体にはなりません。 Gisladottis ら 4およびXu ら 5は、死体の目から収穫硝子体液に透過性を調査します。硝子体抽出のない方法が記載されなかったので、それらはシリンジで硝子体液を吸引することを仮定しました。ワッツら 6は、外科的手法でウサギ硝子体液を単離する方法を説明することによって、さらに一歩行ってきました。しかし、この方法だけではなく、硝子体コア硝子体皮質の単離をもたらします。シェイエら 7は、後に4つのユニークな領域に硝子体を組織し、エレガントな分析のための各部分を分析する方法を説明しました。この技術は、しかしながら、全体としてそのまま硝子体をもたらさありません。 現在の技術は現在、死体の目で行われている生物物理学的実験を容易にするために開発されました。以前の方法は、記載のようにBOVEは、1）いずれも完全に全体の硝子体を分離しないため制限されている、2）収穫硝子体コアと皮質は3）硝子体の解剖学的構造が維​​持されていない、均質化されている、または4）解剖技術が十分に他の分野の研究者による複製のために詳述されていません。また、強​​膜と脈絡膜、硝子体の可視化の不透明度に起因するが、無傷の眼球に制限されています。これは、眼全体の内側にすることができる測定の精度と実現可能性を制限します。また、硝子体の周囲の解剖学的構造は、硝子体の生化学的および物理的性質の研究を混乱することができます。 近年では、硝子科学の本体は飛躍的に成長しており、全体の硝子体は、その個々の部分とは異なる特性を有することを信じる理由があります。 vitreodynamicsのresearcのための硝子体の物理的、生体力学的、および化学的特性を調査への関心の高まりがありますそのような薬物送達、硝子体内酸素8および硝子体切除などの臨床医学への応用を持っている時間、。硝子体を操作するための薬理学的物質を使用して薬理学的vitreodynamicsは、硝子体切除術の成果9を改善するために使用することができます。生体力学的特性は、硝子体内薬物送達技術10-12を改善するために使用することができる硝子体液の流れをモデル化するために使用されます。硝子体の様々なセグメントの物理的特性は、硝子体網膜酸素輸送13を理解するために重要です。提案された硝子体切開法は、無傷硝子体液の種々の特性を研究するために使用することができます。これは、ベンチトップ実験をより見やすくして全体、無傷の硝子体上で行うことを可能にします。 要約すると、硝子体の研究のための現在の方法は、いずれの適切記載されていない、または不完全な分離に硝子体コアと皮質をもたらします。したがって、Eを実行する必要があります透明な眼モデルでxperiments死体の眼に存在する硝子体の解剖学的構造を保持したまま。

<table><tbody><tr><td>0.3 forceps</td><td>Storz Opthalmics</td><td>E1793</td><td></td></tr><tr><td>Westcott Tenotomy Scissors Curved Right</td><td>Storz Opthalmics</td><td>E3320 R</td><td></td></tr><tr><td>Scalpel Handle No. 3</td><td>VWR</td><td>25607-947</td><td></td></tr><tr><td>Scalpel Blade, #11, for #3 Handle</td><td>VWR</td><td>470174-844</td><td></td></tr></tbody></table>

whole vitreous humor dissection for vitreodynamic analysis

The authors propose an effective technique to isolate whole, intact vitreous core and cortex from post mortem enucleated porcine eyes. While previous studies have shown the results of such dissections, the detailed steps have not been described, precluding researchers outside the field from replicating their methods. Other studies harvest vitreous either through aspiration, which does not maintain the vitreous structure anatomy, or through partial dissection, which only isolates the vitreous core. The proposed method isolates the whole vitreous body, with the vitreous core and cortex intact, while maintaining vitreous anatomy and structural integrity. In this method, a full thickness scleral flap in an enucleated porcine eye is first created and through this, the choroid tissue can be separated from the sclera. The scleral flap is then expanded and the choroid is completely separated from the sclera. Finally the choroid-retina tissue is peeled off the vitreous to leave an isolated intact vitreous body. The proposed vitreous dissection technique can be used to study physical properties of the vitreous humor. In particular, this method has significance for experimental studies involving drug delivery, vitreo-retinal oxygen transport, and intraocular convection.

プロトコルに続いて、コアおよび皮質（ 図3）をそのままにして、成功した硝子体切開につながります。これは、硝子体皮質に付着した網膜の残留個から明らかです。無傷の全体硝子体液は、特定のvitreodynamic実験のためのいくつかの方法で使用することができます。我々のケースでは、無傷の硝子体液中の酸素の拡散速度と、それが対応する時定数だが（ 図2）を検?...

Watch this Scientific Journal Video about Whole Vitreous Humor Dissection for Vitreodynamic Analysis at JoVE.com

Whole Vitreous Humor Dissection for Vitreodynamic Analysis

The goal of this protocol is to show an effective technique to isolate whole, intact vitreous core and cortex from post mortem enucleated porcine eyes.

Vitreodynamic分析のための全硝子体液解剖

The authors propose an effective technique to isolate whole, intact vitreous core and cortex from post mortem enucleated porcine eyes. While previous ...

whole-vitreous-humor-dissection-for-vitreodynamic-analysis

Research

JoVE Journal

Medicine

11.3K ビュー.  University of Southern California. The goal of this protocol is to show an effective technique to isolate whole, intact vitreous core and cortex from post mortem enucleated porcine eyes.

ビデオ: Whole Vitreous Humor Dissection for Vitreodynamic Analysis

Dissection of Human Vitreous Body Elements for Proteomic Analysis

The vitreous is an optically clear, collagenous extracellular matrix that fills the inside of the eye and overlies the retina. 1,2 Abnormal interactions between vitreous substructures and the retina underlie several vitreoretinal diseases, including retinal tear and detachment, macular pucker, macular hole, age-related macular degeneration, vitreomacular traction, proliferative vitreoretinopathy, proliferative diabetic retinopathy, and inherited vitreoretinopathies. 1,2 The molecular composition of the vitreous substructures is not known. Since the vitreous body is transparent with limited surgical access, it has been difficult to study its substructures at the molecular level. We developed a method to separate and preserve these tissues for proteomic and biochemical analysis. The dissection technique in this experimental video shows how to isolate vitreous base, anterior hyaloid, vitreous core, and vitreous cortex from postmortem human eyes. One-dimensional SDS-PAGE analyses of each vitreous component showed that our dissection technique resulted in four unique protein profiles corresponding to each substructure of the human vitreous body. Identification of differentially compartmentalized proteins will reveal candidate molecules underlying various vitreoretinal diseases.

This video shows an effective technique for differentiating and dissecting the various semi-transparent structures of the human vitreous body in post mortem eyes.

プロテオーム解析のための人間の硝子体の要素の解剖

The vitreous is an optically clear, collagenous extracellular matrix that fills the inside of the eye and overlies the retina. 1,2 Abnormal interactions ...

医学

無細胞DNAを用いた硝子体網膜リンパ腫の診断

Cell-Free DNA Extraction of Vitreous and Aqueous Humor Specimens for Diagnosis and Monitoring of Vitreoretinal Lymphoma

Vitreoretinal lymphoma (VRL) represents an aggressive lymphoma, often categorized as primary central nervous system diffuse large B-cell lymphoma. To diagnose VRL, specimens such as vitreous humor and, more recently, aqueous humor are collected. Diagnostic testing for VRL on these specimens includes cytology, flow cytometry, and molecular testing. However, both cytopathology and flow cytometry, along with molecular testing using cellular DNA, necessitate intact whole cells. The challenge lies in the fact that vitreous and aqueous humor typically have low cellularity, and many cells get destroyed during collection, storage, and processing. Moreover, these specimens pose additional difficulties for molecular testing due to the high viscosity of vitreous humor and the low volume of both vitreous and aqueous humor. This study proposes a method for extracting cell-free DNA from vitreous and aqueous specimens. This approach complements the extraction of cellular DNA or allows the cellular component of these specimens to be utilized for other diagnostic methods, including cytology and flow cytometry.

著者スポットライト:硝子体液からの無細胞DNA抽出を使用したVRL診断の進歩 

A procedure to extract cell-free DNA from vitreous and aqueous humor to perform molecular studies for diagnosing vitreoretinal lymphoma is established here. The method offers the ability to concurrently extract DNA from the cellular component of the sample or to reserve it for ancillary testing.

網膜硝子体リンパ腫の診断とモニタリングのための硝子体および房水検体の無細胞DNA抽出

Vitreoretinal lymphoma (VRL) represents an aggressive lymphoma, often categorized as primary central nervous system diffuse large B-cell lymphoma. To ...

がん研究

Evisceration of Mouse Vitreous and Retina for Proteomic Analyses

While the mouse retina has emerged as an important genetic model for inherited retinal disease, the mouse vitreous remains to be explored. The vitreous is a highly aqueous extracellular matrix overlying the retina where intraocular as well as extraocular proteins accumulate during disease.1-3 Abnormal interactions between vitreous and retina underlie several diseases such as retinal detachment, proliferative diabetic retinopathy, uveitis, and proliferative vitreoretinopathy.1,4 The relative mouse vitreous volume is significantly smaller than the human vitreous (Figure 1), since the mouse lens occupies nearly 75% of its eye.5 This has made biochemical studies of mouse vitreous challenging. In this video article, we present a technique to dissect and isolate the mouse vitreous from the retina, which will allow use of transgenic mouse models to more clearly define the role of this extracellular matrix in the development of vitreoretinal diseases.

The dissection technique illustrates evisceration of the vitreous, retina, and lens from the mouse eye, separation by centrifugation, and characterization with protein assays.

プロテオーム解析のためのマウスの硝子体と網膜の内臓摘出

While the mouse retina has emerged as an important genetic model for inherited retinal disease, the mouse vitreous remains to be explored. The vitreous is ...

生物学

A Step by Step Protocol for Subretinal Surgery in Rabbits

Age related macular degeneration (AMD), retinitis pigmentosa, and other RPE related diseases are the most common causes for irreversible loss of vision in adults in industrially developed countries. RPE transplantation appears to be a promising therapy, as it may replace dysfunctional RPE, restore its function, and thereby vision.
Here we describe a method for transplanting a cultured RPE monolayer on a scaffold into the subretinal space (SRS) of rabbits. After vitrectomy xenotransplants were delivered into the SRS using a custom made shooter consisting of a 20-gauge metallic nozzle with a polytetrafluoroethylene (PTFE) coated plunger. The current technique evolved in over 150 rabbit surgeries over 6 years. Post-operative follow-up can be obtained using non-invasive and repetitive in vivo imaging such as spectral domain optical coherence tomography (SD-OCT) followed by perfusion-fixed histology.
The method has well-defined steps for easy learning and high success rate. Rabbits are considered a large eye animal model useful in preclinical studies for clinical translation. In this context rabbits are a cost-efficient and perhaps convenient alternative to other large eye animal models.

Retinal pigment epithelium (RPE) replacement strategies and gene-based therapy are considered for several retinal degenerative conditions. For clinical translation, large eye animal models are required to study surgical techniques applicable in patients. Here we present a rabbit model for subretinal surgery geared towards RPE transplantation, which is versatile and cost-efficient.

ウサギにおける網膜下手術のためのステッププロトコルによってステップ

Age related macular degeneration (AMD), retinitis pigmentosa, and other RPE related diseases are the most common causes for irreversible loss of vision in ...

Microsurgical Dissection and Tissue Clearing for High Resolution Intact Whole Retina and Vitreous Imaging

Neuronal and vascular structures of the retina in physiologic and pathologic conditions can be better visualized and characterized by using intact whole retina imaging techniques compared to conventional retinal flat mount preparations and sections. However, immunofluorescent imaging of intact whole retina is hindered by the opaque coatings of the eyeball, i.e., sclera, choroid, and retinal pigment epithelium (RPE) and the light scattering properties of retinal layers that prevent full thickness high resolution optical imaging. Chemical bleaching of the pigmented layers and tissue clearing protocols have been described to address these obstacles; however, currently described methods are not suitable for imaging endogenous fluorescent molecules such as green fluorescent protein (GFP) in intact whole retina. Other approaches bypassed this limitation by surgical removal of pigmented layers and the anterior segment of the eyeball allowing intact eye imaging, though the peripheral retina and hyaloid structures were disrupted. Presented here is an intact whole retina and vitreous immunofluorescent imaging protocol that combines surgical dissection of the sclera/choroid/retina pigment epithelium (RPE) layers with a modified tissue clearing method and light sheet fluorescent microscopy (LSFM). The new approach offers an unprecedented view of&#160;unperturbed vascular and neuronal elements of the retina as well as the vitreous and hyaloid vascular system in pathologic conditions.

Presented here is a protocol for intact whole retina imaging in which the outer opaque/pigmented layers of the eyeball are surgically removed, and optical clearing is applied to render retina transparent enabling the visualization of the peripheral retina and hyaloid vasculature in intact retina using light sheet fluorescent microscopy.

Neuronal and vascular structures of the retina in physiologic and pathologic conditions can be better visualized and characterized by using intact whole ...

神経科学

Full-Circle Cauterization of Limbal Vascular Plexus for Surgically Induced Glaucoma in Rodents

Glaucoma, the second leading cause of blindness worldwide, is a heterogeneous group of ocular disorders characterized by structural damage to the optic nerve and retinal ganglion cell (RGC) degeneration, resulting in visual dysfunction by interrupting the transmission of visual information from the eye to the brain. Elevated intraocular pressure is the most important risk factor; thus, several models of ocular hypertension have been developed in rodents by either genetic or experimental approaches to investigate the causes and effects of the disease. Among those, some limitations have been reported such as surgical invasiveness, inadequate functional assessment, requirement of extensive training, and highly variable extension of retinal damage. The present work characterizes a simple, low-cost, and efficient method to induce ocular hypertension in rodents, based on low-temperature, full-circle cauterization of the limbal vascular plexus, a major component of aqueous humor drainage. The new model provides a technically easy, noninvasive, and reproducible subacute ocular hypertension, associated with progressive RGC and optic nerve degeneration, and a unique post-operative clinical recovery rate that allows in vivo functional studies by both electrophysiological and behavioral methods.

The goal of this protocol is to characterize a novel model of glaucomatous neurodegeneration based on 360&#176; thermic cauterization of limbal vascular plexus, inducing subacute ocular hypertension.

げっ歯類の外科的緑内障に対する辺縁血管神経叢の全周焼灼

Glaucoma, the second leading cause of blindness worldwide, is a heterogeneous group of ocular disorders characterized by structural damage to the optic ...

Retinal Pigment Epithelium Transplantation in a Non-human Primate Model for Degenerative Retinal Diseases

Retinal pigment epithelial (RPE) transplantation holds great promise for the treatment of inherited and acquired retinal degenerative diseases. These conditions include retinitis pigmentosa (RP) and advanced forms of age-related macular degeneration (AMD), such as geographic atrophy (GA). Together, these disorders represent a significant proportion of currently untreatable blindness globally. These unmet medical needs have generated heightened academic interest in developing methods of RPE replacement. Among the animal models commonly utilized for preclinical testing of therapeutics, the non-human primate (NHP) is the only animal model that has a macula. As it shares this anatomical similarity with the human eye, the NHP eye is an important and appropriate preclinical animal model for the development of advanced therapy medicinal products (ATMPs) such as RPE cell therapy.
This manuscript describes a method for the submacular transplantation of an RPE monolayer, cultured on a polyethylene terephthalate (PET) cell carrier, underneath the macula onto a surgically created RPE wound in immunosuppressed NHPs. The fovea-the central avascular portion of the macula-is the site of the greatest mechanical weakness during the transplantation. Foveal trauma will occur if the initial subretinal fluid injection generates an excessive force on the retina. Hence, slow injection under perfluorocarbon liquid (PFCL) vitreous tamponade is recommended with a dual-bore subretinal injection cannula at low intraocular pressure (IOP) settings to create a retinal bleb. 
Pretreatment with an intravitreal plasminogen injection to release parafoveal RPE-photoreceptor adhesions is also advised. These combined strategies can reduce the likelihood of foveal tears when compared to conventional techniques. The NHP is a key animal model in the preclinical phase of RPE cell therapy development. This protocol addresses the technical challenges associated with the delivery of RPE cellular therapy in the NHP eye.

The non-human primate (NHP) is an ideal model for studying human retinal cellular therapeutics due to the anatomical and genetic similarities. This manuscript describes a method for submacular transplantation of retinal pigment epithelial cells in the NHP eye and strategies to prevent intraoperative complications associated with macular manipulation.

ヒト以外の霊長類モデルにおける変性網膜疾患の網膜色素上皮移植

Retinal pigment epithelial (RPE) transplantation holds great promise for the treatment of inherited and acquired retinal degenerative diseases. These ...

AMD研究のためのヒトドナー眼のOCTベースマルチモーダルイメージング

Ex Vivo OCT-Based Multimodal Imaging of Human Donor Eyes for Research into Age-Related Macular Degeneration

A progression sequence for age-related macular degeneration (AMD) learned from optical coherence tomography (OCT)-based multimodal (MMI) clinical imaging could add prognostic value to laboratory findings. In this work, ex vivo OCT and MMI were applied to human donor eyes prior to retinal tissue sectioning. The eyes were recovered from non-diabetic white donors aged &#8805;80 years old, with a death-to-preservation time (DtoP) of &#8804;6 h. The globes were recovered on-site, scored with an 18 mm trephine to facilitate cornea removal, and immersed in buffered 4% paraformaldehyde. Color fundus images were acquired after anterior segment removal with a dissecting scope and an SLR camera using trans-, epi-, and flash illumination at three magnifications. The globes were placed in a buffer within a custom-designed chamber with a 60 diopter lens. They were imaged with spectral domain OCT (30&#176; macula cube, 30 &#181;m spacing, averaging = 25), near-infrared reflectance, 488 nm autofluorescence, and 787 nm autofluorescence. The AMD eyes showed a change in the retinal pigment epithelium (RPE), with drusen or subretinal drusenoid deposits (SDDs), with or without neovascularization, and without evidence of other causes. Between June 2016 and September 2017, 94 right eyes and 90 left eyes were recovered (DtoP: 3.9 &plusmn; 1.0 h). Of the 184 eyes, 40.2% had AMD, including early intermediate (22.8%), atrophic (7.6%), and neovascular (9.8%) AMD, and 39.7% had unremarkable maculas. Drusen, SDDs, hyper-reflective foci, atrophy, and fibrovascular scars were identified using OCT. Artifacts included tissue opacification, detachments (bacillary, retinal, RPE, choroidal), foveal cystic change, an undulating RPE, and mechanical damage. To guide the cryo-sectioning, OCT volumes were used to find the fovea and optic nerve head landmarks and specific pathologies. The ex vivo volumes were registered with the in vivo volumes by selecting the reference function for eye tracking. The ex vivo visibility of the pathology seen in vivo depends on the preservation quality. Within 16 months, 75 rapid DtoP donor eyes at all stages of AMD were recovered and staged using clinical MMI methods.

著者スポットライト:加齢性黄斑変性症の研究のためのヒトドナー眼のEx Vivo OCTベースのマルチモーダルイメージング  

Laboratory assays can leverage prognostic value from the longitudinal optical coherence tomography (OCT)-based multimodal imaging of age-related macular degeneration (AMD). Human donor eyes with and without AMD are imaged using OCT, color, near-infrared reflectance scanning laser ophthalmoscopy, and autofluorescence at two excitation wavelengths prior to tissue sectioning.

エクスビボ 加齢黄斑変性症研究のためのヒトドナー眼のOCTを用いたマルチモーダルイメージング

A progression sequence for age-related macular degeneration (AMD) learned from optical coherence tomography (OCT)-based multimodal (MMI) clinical imaging ...

Vitreodynamic分析のための全硝子体液解剖

要約

さらに動画を探す

この動画の章

プロテオーム解析のための人間の硝子体の要素の解剖

網膜硝子体リンパ腫の診断とモニタリングのための硝子体および房水検体の無細胞DNA抽出

網膜硝子体リンパ腫の診断とモニタリングのための硝子体および房水検体の無細胞DNA抽出

網膜硝子体リンパ腫の診断とモニタリングのための硝子体および房水検体の無細胞DNA抽出

プロテオーム解析のためのマウスの硝子体と網膜の内臓摘出

ウサギにおける網膜下手術のためのステッププロトコルによってステップ

Microsurgical Dissection and Tissue Clearing for High Resolution Intact Whole Retina and Vitreous Imaging

げっ歯類の外科的緑内障に対する辺縁血管神経叢の全周焼灼

ヒト以外の霊長類モデルにおける変性網膜疾患の網膜色素上皮移植

エクスビボ加齢黄斑変性症研究のためのヒトドナー眼のOCTを用いたマルチモーダルイメージング

Vitreodynamic分析のための全硝子体液解剖

要約

さらに動画を探す

この動画の章

プロテオーム解析のための人間の硝子体の要素の解剖

網膜硝子体リンパ腫の診断とモニタリングのための硝子体および房水検体の無細胞DNA抽出

網膜硝子体リンパ腫の診断とモニタリングのための硝子体および房水検体の無細胞DNA抽出

網膜硝子体リンパ腫の診断とモニタリングのための硝子体および房水検体の無細胞DNA抽出

プロテオーム解析のためのマウスの硝子体と網膜の内臓摘出

ウサギにおける網膜下手術のためのステッププロトコルによってステップ

Microsurgical Dissection and Tissue Clearing for High Resolution Intact Whole Retina and Vitreous Imaging

げっ歯類の外科的緑内障に対する辺縁血管神経叢の全周焼灼

ヒト以外の霊長類モデルにおける変性網膜疾患の網膜色素上皮移植

エクスビボ 加齢黄斑変性症研究のためのヒトドナー眼のOCTを用いたマルチモーダルイメージング

エクスビボ加齢黄斑変性症研究のためのヒトドナー眼のOCTを用いたマルチモーダルイメージング