Pooled shRNA Screen for Reactivation of MeCP2 on the Inactive X Chromosome

1:17

Selecting Single Cell Clones and Characterizing MeCP2 Reporter Expression

4:31

Screening the Library for Reactivation Using Hygromycin B Selection

6:44

Validating the Identified Hairpins ( Hits )

9:27

Results: Isolation of Clones with the Reporter on the Inactive X Chromosome and Characterization of Hygromycin Resistance

10:17

Conclusion