This method can help answer key questions in the cancer metastasis field about the role of lung colonization in accomplishing cancer metastasis. The main advantage of this technique is that early extravasated cancer cells can be visualized in the
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An animal model is needed to decipher the role of circulating tumor cells (CTCs) in promoting lung colonization during cancer metastasis. Here, we established and successfully performed an in vivo assay to specifically test the requirement of polymeric fibronectin (polyFN) assembly on CTCs for lung colonization.