すべての同僚の貢献と (説明のため) この原稿に呼ばれている、結果が私たちの元の研究に協力したいと思います。作家の作品は、国立歯科・頭蓋顔面研究、国立衛生研究所 (1R01DE026043) ab の AB (California Tobacco-Related 病研究プログラムの大学からの補助金によってサポートされてTRDRP-26IR-0015) および ST (TRDRP-25IP-0001)。研究のスポンサーには、研究デザイン、データ収集、データ分析、データ解釈、レポートの執筆、出版のために提出する決定の役割はなかった。

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Λ 選択cIIアッセイは、3BB の齧歯動物の臓器・組織由来細胞のゲノム DNA から回復したcII遺伝子変異の検出に使用されます。これらの形質転換動物ゲノムには染色体統合 λLIZ シャトル ベクトルは、 cIIを運ぶ複数タンデム コピーが含まれます (294 bp) とラッチ(1,080 bp) 遺伝子変異レポーター遺伝子1,2,</su...

トランスジェニック動物モデルおよび突然変異検出システムの広い範囲は、哺乳類細胞における発がん性物質の変異原性試験のために開発されています。これらの大きなブルー (以下 bb) トランスジェニック マウスと λ 選択cII変異検出システムで採用されている変異原性実験のためこのグループは、多くの他研究グループ世界中1,の2、 3,4,5,6,7,8,9。過去 16 年間、これらのトランスジェニック動物を用いた化学的および/または物理的な剤の変異原性の効果を調べたか、対応する胚性線維芽細胞培養テスト混合物と扱われ、その後の分析、λ 選択cIIアッセイおよび DNA の配列、それぞれ10、11,12,の13,14によってcII遺伝子の遺伝子型と表現型,15,16,17,18,19,20,21,22,23,24. これらのトランスジェニック動物のゲノムに複数コピー頭-尾コンカテマー1,2,25, 染色体 4 統合バクテリオファージ λ シャトル ベクトル (λLIZ) が含まれています。ΛLIZ シャトルベクターを運ぶ 2 つの変異レポーター遺伝子、すなわち、ラッチとcII遺伝子1,2,25,26,27、 28,29,30,31,32,33,34,35,36、 37,38,39,40,41,42,43,44,45,46,47. λ 選択cIIアッセイに基づいて λLIZ シャトル ベクトルのトランスジェニック動物1,2,25 の臓器・組織由来細胞のゲノム DNA からの回復.回復 λLIZ シャトル ベクトル、インジケーター ホスト大腸菌に感染可能な λ ファージの頭にパッケージ化されます。その後、感染した細菌は、得点とcII遺伝子1,3における変異解析を可能にする選択的な条件のもとで栽培しています。
ここ、体外治療トランスジェニック動物の細胞・臓器からゲノム DNA の隔離から成っている λ 選択cIIの試金のための詳しいプロトコルについて述べる/シャトル生体内でλLIZ の化合物は、取得テスト ベクトルgenomic DNA から、バクテリオファージ、 cIIの同定とホストエシェリヒア属大腸菌の感染感染 λ ファージにベクトルの包装- cIIの突然変異頻度を決定する選択的な条件の下で突然変異体とCII変異スペクトルを確立する dna 塩基配列解析。トランスジェニック マウス ・ ラット細胞培養体外興味の化学/物理エージェントに適用できるプロトコルまたは対応する動物の組織/臓器治療体内テスト化学/エージェント1、 2,4,48,49,50,51,52。Λ 選択cIIアッセイの図式を図 1に示します。

<table><tbody><tr><td>Agar</td><td>MO Bio Laboratories, Inc.</td><td>12112-05</td><td>Bacteriological grade</td></tr><tr><td>BigDye Terminator v3.1 Cycle Sequencing Kit</td><td>Thermo Fisher Scientific</td><td>4337455</td><td>None</td></tr><tr><td>Casein Peptone</td><td>Alfa Aesar</td><td>H26557</td><td>None</td></tr><tr><td>Gelatine</td><td>J. T. Baker</td><td>2124-01</td><td>Powder</td></tr><tr><td>Glycerol</td><td>Fisher Scientific</td><td>BP 229-1 / M-13750</td><td>None</td></tr><tr><td>LB Agar</td><td>Fisher Scientific</td><td>BP 9724-500</td><td>None</td></tr><tr><td>QIAquick PCR purification kit</td><td>Qiagen</td><td>8104</td><td>50 PCR purification reactions</td></tr><tr><td>Sodium Acetate Trihydrate</td><td>Fisher Scientific</td><td>M-15756</td><td>None</td></tr><tr><td>&lt;em&gt;Taq5000&lt;/em&gt; DNA Polymerase&amp;nbsp;</td><td>Qiagen</td><td>201207</td><td>None</td></tr><tr><td>Thiamine Hydrochloride</td><td>Macron Fine Chemicals</td><td>2722-57</td><td>None</td></tr><tr><td>Transpack Packaging Extract</td><td>Stratagene Corp., Acquired by BioReliance | Sigma-Aldrich Corp.</td><td>200223</td><td>50 packaging reactions</td></tr><tr><td>Tris Base</td><td>Fisher Scientific</td><td>BP 152-1 / EC 201-064-4</td><td>None</td></tr><tr><td>Trypton</td><td>Biosciences</td><td>RC-110</td><td>None</td></tr></tbody></table>

the lambda select cii mutation detection system

トランスジェニック動物モデルおよび突然変異検出システムの数は、哺乳類細胞における発がん性物質の変異原性試験のために開発されています。これらのトランスジェニック マウスとラムダ (λ) の選択cII変異検出システム世界中の多くの研究グループによって変異原性実験のため採用されています。ここでは、ラムダを選択cII突然変異試験は、トランスジェニック マウス/ラットの培養細胞に適用することができるための詳しいプロトコルについて述べるまたは興味の化学/物理エージェントに対応する動物が扱われます。プロトコルは、次の手順で構成されます: (1) 分離細胞からゲノム dna やトランスジェニック動物の臓器・組織生体外でまたは生体内で、それぞれで治療テスト混合物;(ゲノム DNA からの (すなわち、 cII遺伝子) の変異レポーター遺伝子を運ぶラムダ シャトルベクターの回復 2)(伝染性のバクテリオファージに救助されたベクトルの包装 3)(4) ホストの細菌に感染して誘導cII突然変異の伝播を許可する選択的な条件下で培養(5) 得点cII-変異体と DNA シーケンスをそれぞれcII突然変異頻度および突然変異スペクトルを分析します。

データ ディストリビューションによってパラメトリックまたはノンパラのテストを使用して、処置および制御グループ (すなわち、自発の突然変異体の周波数対誘導) のcIIの突然変異頻度の違いの重要性を判断します.誘導cII変異周波数別の治療グループ間での比較が行われます各種 (一対)、適用可能な統計的テスト。Χ2テストや分散分析 (...

Watch this Scientific Journal Video about The Lambda Select cII Mutation Detection System at JoVE.com

The Lambda Select cII Mutation Detection System

ラムダを選択cII変異検出システム

トランスジェニックげっ歯類の培養細胞でラムダを選択cII突然変異試験または興味の化学/物理エージェントで治療対応する動物のための詳しいプロトコルについて述べる。このアプローチは、哺乳類細胞における発がん性物質の変異原性試験のため広く使用されています。

A number of transgenic animal models and mutation detection systems have been developed for mutagenicity testing of carcinogens in mammalian cells. Of ...

the-lambda-select-cii-mutation-detection-system

Research

JoVE Journal

Immunology and Infection

The Lambda Select cII Mutation Detection System

7.9K ビュー.  University of Southern California. トランスジェニックげっ歯類の培養細胞でラムダを選択cII突然変異試験または興味の化学/物理エージェントで治療対応する動物のための詳しいプロトコルについて述べる。このアプローチは、哺乳類細胞における発がん性物質の変異原性試験のため広く使用されています。

ビデオ: The Lambda Select cII Mutation Detection System

Rare Event Detection Using Error-corrected DNA and RNA Sequencing

Conventional next-generation sequencing techniques (NGS) have allowed for immense genomic characterization for over a decade. Specifically, NGS has been used to analyze the spectrum of clonal mutations in malignancy. Though far more efficient than traditional Sanger methods, NGS struggles with identifying rare clonal and subclonal mutations due to its high error rate of ~0.5&#8211;2.0%. Thus, standard NGS has a limit of detection for mutations that are &#62;0.02 variant allele fraction (VAF). While the clinical significance for mutations this rare in patients without known disease remains unclear, patients treated for leukemia have significantly improved outcomes when residual disease is &#60;0.0001 by flow cytometry. In order to mitigate this artefactual background of NGS, numerous methods have been developed. Here we describe a method for Error-corrected DNA and RNA Sequencing (ECS), which involves tagging individual molecules with both a 16 bp random index for error-correction and an 8 bp patient-specific index for multiplexing. Our method can detect and track clonal mutations at variant allele fractions (VAFs) two orders of magnitude lower than the detection limit of NGS and as rare as 0.0001 VAF.

Next-generation sequencing (NGS) is a powerful tool for genomic characterization that is limited by the high error rate of the platform (~0.5&#8211;2.0%). We describe our methods of error-corrected sequencing that allow us to obviate the NGS error rate and detect mutations at variant allele fractions as rare as 0.0001.

エラー修正 DNA ・ RNA シーケンスを用いた珍しいイベント検出

Conventional next-generation sequencing techniques (NGS) have allowed for immense genomic characterization for over a decade. Specifically, NGS has been ...

遺伝学

Site Directed Spin Labeling and EPR Spectroscopic Studies of Pentameric Ligand-Gated Ion Channels

Ion channel gating is a stimulus-driven orchestration of protein motions that leads to transitions between closed, open, and desensitized states. Fundamental to these transitions is the intrinsic flexibility of the protein, which is critically modulated by membrane lipid-composition. To better understand the structural basis of channel function, it is necessary to study protein dynamics in a physiological membrane environment. Electron Paramagnetic Resonance (EPR) spectroscopy is an important tool to characterize conformational transitions between functional states. In comparison to NMR and X-ray crystallography, the information obtained from EPR is intrinsically of lower resolution. However, unlike in other techniques, in EPR there is no upper-limit to the molecular weight of the protein, the sample requirements are significantly lower, and more importantly the protein is not constrained by the crystal lattice forces. Therefore, EPR is uniquely suited for studying large protein complexes and proteins in reconstituted systems. In this article, we will discuss general protocols for site-directed spin labeling and membrane reconstitution using a prokaryotic proton-gated pentameric Ligand-Gated Ion Channel (pLGIC) from Gloeobacter violaceus (GLIC) as an example. A combination of steady-state Continuous Wave (CW) and Pulsed (Double Electron Electron Resonance-DEER) EPR approaches will be described that will enable a complete quantitative characterization of channel dynamics.

This article describes methods for site-directed spin labeling and reconstitution of pentameric ligand-gated channels for Electron Paramagnetic Resonance studies. This protocol can be adapted for any membrane protein. The reconstitution method described here can also be used for patch-clamp measurements of macroscopic and single-channel currents in a defined lipid system.

サイト監督スピン標識および五量体リガンド依存性イオンチャネルのEPR分光研究

Ion channel gating is a stimulus-driven orchestration of protein motions that leads to transitions between closed, open, and desensitized states. ...

化学

qKAT: Quantitative Semi-automated Typing of Killer-cell Immunoglobulin-like Receptor Genes

Killer cell immunoglobulin-like receptors (KIRs) are a set of inhibitory and activating immune receptors, on natural killer (NK) and T cells, encoded by a polymorphic cluster of genes on chromosome 19. Their best-characterized ligands are the human leukocyte antigen (HLA) molecules that are encoded within the major histocompatibility complex (MHC) locus on chromosome 6. There is substantial evidence that they play a significant role in immunity, reproduction, and transplantation, making it crucial to have techniques that can accurately genotype them. However, high-sequence homology, as well as allelic and copy number variation, make it difficult to design methods that can accurately and efficiently genotype all KIR genes. Traditional methods are usually limited in the resolution of data obtained, throughput, cost-effectiveness, and the time taken for setting up and running the experiments. We describe a method called quantitative KIR semi-automated typing (qKAT), which is a high-throughput multiplex real-time polymerase chain reaction method that can determine the gene copy numbers for all genes in the KIR locus. qKAT is a simple high-throughput method that can provide high-resolution KIR copy number data, which can be further used to infer the variations in the structurally polymorphic haplotypes that encompass them. This copy number and haplotype data can be beneficial for studies on large-scale disease associations, population genetics, as well as investigations on expression and functional interactions between KIR and HLA.

Quantitative killer cell immunoglobulin-like receptor (KIR) semi-automated typing (qKAT) is a simple, high-throughput, and cost-effective method to copy number type KIR genes for their application in population and disease association studies.

qKAT: キラー細胞免疫グロブリン様受容体遺伝子の定量的半自動化された入力

Killer cell immunoglobulin-like receptors (KIRs) are a set of inhibitory and activating immune receptors, on natural killer (NK) and T cells, encoded by a ...

Identifying Mutations by High Resolution Melting in a TILLING Population of Rice

Target Induced Local Lesions In Genomes (TILLING) is a strategy of reverse genetics for the high-throughput screening of induced mutations. However, the TILLING system has less applicability for insertion/deletion (Indel) detection and traditional TILLING needs more complex steps, like CEL I nuclease digestion and gel electrophoresis. To improve the throughput and selection efficiency, and to make the screening of both Indels and single base substitions (SBSs) possible, a new high-resolution melting (HRM)-based TILLING system is developed. Here, we present a detailed HRM-TILLING protocol and show its application in mutation screening. This method can analyze the mutations of PCR amplicons by measuring the denaturation of double-stranded DNA at high temperatures. HRM analysis is directly performed post-PCR without additional processing. Moreover, a simple, safe and fast (SSF) DNA extraction method is integrated with HRM-TILLING to identify both Indels and SBSs. Its simplicity, robustness and high throughput make it potentially useful for mutation scanning in rice and other crops.

In this article, we present the protocol that is described as high-resolution melting analysis (HRM)-based Target Induced Local Lesions In Genomes (TILLING). This method utilizes fluorescence changes during the melting of the DNA duplex and is suitable for high-throughput screening of both insertion/deletion (Indel) and single base substition (SBS).

米の耕作集団における高解像度溶融による変異の同定

Target Induced Local Lesions In Genomes (TILLING) is a strategy of reverse genetics for the high-throughput screening of induced mutations. However, the ...

Identifying DNA Mutations in Purified Hematopoietic Stem/Progenitor Cells

In recent years, it has become apparent that genomic instability is tightly related to many developmental disorders, cancers, and aging. Given that stem cells are responsible for ensuring tissue homeostasis and repair throughout life, it is reasonable to hypothesize that the stem cell population is critical for preserving genomic integrity of tissues. Therefore, significant interest has arisen in assessing the impact of endogenous and environmental factors on genomic integrity in stem cells and their progeny, aiming to understand the etiology of stem-cell based diseases.
LacI transgenic mice carry a recoverable &#955;&#160;phage vector encoding the LacI reporter system, in which the LacI gene serves as the mutation reporter. The result of a mutated LacI gene is the production of &#946;-galactosidase that cleaves a chromogenic substrate, turning it blue. The LacI reporter system is carried in all cells, including stem/progenitor cells and can easily be recovered and used to subsequently infect E. coli. After incubating infected E. coli on agarose that contains the correct substrate, plaques can be scored; blue plaques indicate a mutant LacI gene, while clear plaques harbor wild-type. The frequency of blue (among clear) plaques indicates the mutant frequency in the original cell population the DNA was extracted from. Sequencing the mutant LacI gene will show the location of the mutations in the gene and the type of mutation.
The LacI transgenic mouse model is well-established as an in vivo mutagenesis assay. Moreover, the mice and the reagents for the assay are commercially available. Here we describe in detail how this model can be adapted to measure the frequency of spontaneously occurring DNA mutants in stem cell-enriched Lin-IL7R-Sca-1+cKit++(LSK) cells and other subpopulations of the hematopoietic system.

Here we describe an in vivo mutagenesis assay for small numbers of purified hematopoietic cells using the LacI transgenic mouse model.&#160;The LacI gene can be isolated to determine the frequency, location, and type of DNA mutants&#160;spontaneously arisen or after exposure to genotoxins.

精製造血幹細胞/前駆細胞のDNAの変異を特定する

In recent years, it has become apparent that genomic instability is tightly related to many developmental disorders, cancers, and aging. Given that stem ...

免疫・感染学

InSET: A Novel Tool for Unbiased Genome-Wide Functional Element Discovery

Identification of Functionally-Relevant Lentivirus Integration Sites in an Insertional Mutagenesis Cell Library

The extent of functional sequences within the human genome is a pivotal yet debated topic in biology. Although high-throughput reverse genetic screens have made strides in exploring this, they often limit their scope to known genomic elements and may introduce non-specific effects. This underscores the urgent need for novel functional genomics tools that enable a deeper, unbiased understanding of genome functionality. This protocol introduces the Insertion-based Screen for functional Elements and Transcripts (InSET), a method for identifying lentivirus integration sites within a lentivirus-based insertional mutagenesis cell library. InSET facilitates the capture of genome-wide lentiviral integration sites, with next-generation sequencing used to detect and quantify flanking sequences. InSET's design enables the analysis of integration site abundance variations in phenotypic screens on a large scale, establishing it as a robust tool for forward genetics and for identifying functional genomic elements. A key benefit of InSET is its capacity to reveal previously unidentified genomic elements, including novel functional exons of both protein-coding and non-coding RNAs, independent of prior annotation. Overall, InSET holds significant value in studying the intricate complexity of the human genome and transcriptome, where many genomic elements await functional characterization.

Insertional mutagenesis is an essential tool in forward genetics for identifying functional genomic elements. Here, we describe the Insertion-based Screen for functional Elements and Transcripts (InSET), a method for detecting lentivirus integration sites within a lentivirus-based insertional mutagenesis cell library.

The extent of functional sequences within the human genome is a pivotal yet debated topic in biology. Although high-throughput reverse genetic screens ...

Mutagenesis and Functional Selection Protocols for Directed Evolution of Proteins in E. coli

The efficient generation of genetic diversity represents an invaluable molecular tool that can be used to label DNA synthesis, to create unique molecular signatures, or to evolve proteins in the laboratory. Here, we present a protocol that allows the generation of large (>1011) mutant libraries for a given target sequence. This method is based on replication of a ColE1 plasmid encoding the desired sequence by a low-fidelity variant of DNA polymerase I (LF-Pol I). The target plasmid is transformed into a mutator strain of E. coli and plated on solid media, yielding between 0.2 and 1 mutations/kb, depending on the location of the target gene. Higher mutation frequencies are achieved by iterating this process of mutagenesis. Compared to alternative methods of mutagenesis, our protocol stands out for its simplicity, as no cloning or PCR are involved. Thus, our method is ideal for mutational labeling of plasmids or other Pol I templates or to explore large sections of sequence space for the evolution of activities not present in the original target. The tight spatial control that PCR or randomized oligonucleotide-based methods offer can also be achieved through subsequent cloning of specific sections of the library. Here we provide protocols showing how to create a random mutant library and how to establish drug-based selections in E. coli to identify mutants exhibiting new biochemical activities.

Mutagenesis and Functional Selection Protocols for Directed Evolution of Proteins in E. coli

Here we demonstrate a simple protocol to create a random mutant library for a given target sequence. We show how this method, which is performed in vivo in Escherichia coli, can be coupled with functional selections to evolve new enzymatic activities.

内タンパク質の定方向進化のための変異導入と機能選択プロトコル E.大腸菌</em

The efficient generation of genetic diversity represents an invaluable molecular tool that can be used to label DNA synthesis, to create unique molecular ...

生物学

Field-Deployable Candidatus Liberibacter asiaticus Detection Using Recombinase Polymerase Amplification Combined with CRISPR-Cas12a

The early detection of Candidatus Liberibacter asiaticus (CLas) by citrus growers facilitates early intervention and prevents the spread of disease. A simple method for rapid and portable Huanglongbing (HLB) diagnosis is presented here that combines recombinase polymerase amplification and a fluorescent reporter utilizing the nuclease activity of the clustered regularly interspaced short palindromic repeats/CRISPR-associated 12a (CRISPR-Cas12a) system. The sensitivity of this technique is much higher than PCR. Furthermore, this method showed similar results to qPCR when leaf samples were used. Compared with conventional CLas detection methods, the detection method presented here can be completed in 90 min and works in an isothermal condition that does not require the use of PCR machines. In addition, the results can be visualized through a handheld fluorescent detection device in the field.

Field-Deployable Candidatus Liberibacter asiaticus Detection Using Recombinase Polymerase Amplification Combined with CRISPR-Cas12a

In this work, a rapid, sensitive, and portable detection method for Candidatus Liberibacter asiaticus based on recombinase polymerase amplification combined with CRISPR-Cas12a was developed.

CRISPR-Cas12aと組み合わせたリコンビナーゼポリメラーゼ増幅を用いたフィールド展開可能な カンディダトゥス ・リベリバクター・アジアティカス検出(英語)

The early detection of Candidatus Liberibacter asiaticus (CLas) by citrus growers facilitates early intervention and prevents the spread of disease. A ...

Following the Dynamics of Structural Variants in Experimentally Evolved Populations

Structural variants (SVs) (i.e., deletions, insertions, duplications, and inversions) are now known to play an important role in phenotypic variation, and consequently in processes such as disease determination or adaptation to a new environment. However, single-nucleotide variants receive much more attention than SVs, probably because they are easier to detect, and their phenotypic effects are easier to predict. The development of short- and long-read deep sequencing technologies have strongly improved the detection of SVs, but the quantification of their frequency from pooled sequencing (poolseq) data is still technically complex and expensive.
Here, we present a rather simple and inexpensive method, which allows researchers to follow the dynamics of SV allele frequency. As an example of application, we follow the frequency of an insertion sequence (IS) insertion in experimental evolution populations of bacteria. This method is based on the design of triplets of primers around the structural variant borders, such that the amplicons produced by amplification of the wild-type (WT) and derived alleles differ in size by at least 5%, and that their amplification efficiency is similar. The quantity of each amplicon is then determined by parallel capillary electrophoresis and normalized to a calibration curve. This method can be easily extended to the quantification of the frequency of other structural variants (deletions, duplications, and inversions) and to pool-seq approaches of natural populations, including within-patient pathogen populations.

We developed a cost-effective method to follow non-single nucleotide polymorphism allele dynamics that can easily be adapted to experimental evolution frozen archives. A triplet PCR technique was coupled with automated parallel capillary electrophoresis to quantify the relative frequency of an insertion allele over the course of experimental evolution.

実験的に進化した集団における構造変異のダイナミクスの追跡

Structural variants (SVs) (i.e., deletions, insertions, duplications, and inversions) are now known to play an important role in phenotypic variation, and ...

DNA Virus Detection System Based on RPA-CRISPR/Cas12a-SPM and Deep Learning

We report a fast, easy-to-implement, highly sensitive, sequence-specific, and point-of-care (POC) DNA virus detection system, which combines recombinase polymerase amplification (RPA) and CRISPR/Cas12a system for trace detection of DNA viruses. Target DNA is amplified and recognized by RPA and CRISPR/Cas12a separately, which triggers the collateral cleavage activity of Cas12a that cleaves a fluorophore-quencher labeled DNA reporter and generalizes fluorescence. For POC detection, portable smartphone microscopy is built to take fluorescent images. Besides, deep learning models for binary classification of positive or negative samples, achieving high accuracy, are deployed within the system. Frog virus 3 (FV3, genera Ranavirus, family Iridoviridae) was tested as an example for this DNA virus POC detection system, and the limits of detection (LoD) can achieve 10 aM within 40 min. Without skilled operators and bulky instruments, the portable and miniature RPA-CRISPR/Cas12a-SPM with artificial intelligence (AI) assisted classification shows great potential for POC DNA virus detection and can help prevent the spread of such viruses.

We present a protocol that combines recombinase polymerase amplification with a CRISPR/Cas12a system for trace detection of DNA viruses and builds portable smartphone microscopy with an artificial intelligence-assisted classification for point-of-care DNA virus detection.

RPA-CRISPR/Cas12A-SPMとディープラーニングに基づくDNAウイルス検出システム

We report a fast, easy-to-implement, highly sensitive, sequence-specific, and point-of-care (POC) DNA virus detection system, which combines recombinase ...

ラムダを選択cII変異検出システム

要約

さらに動画を探す

エラー修正 DNA ・ RNA シーケンスを用いた珍しいイベント検出

サイト監督スピン標識および五量体リガンド依存性イオンチャネルのEPR分光研究

qKAT: キラー細胞免疫グロブリン様受容体遺伝子の定量的半自動化された入力

米の耕作集団における高解像度溶融による変異の同定

精製造血幹細胞/前駆細胞のDNAの変異を特定する

Identification of Functionally-Relevant Lentivirus Integration Sites in an Insertional Mutagenesis Cell Library

内タンパク質の定方向進化のための変異導入と機能選択プロトコル E.大腸菌

CRISPR-Cas12aと組み合わせたリコンビナーゼポリメラーゼ増幅を用いたフィールド展開可能なカンディダトゥス・リベリバクター・アジアティカス検出(英語)

実験的に進化した集団における構造変異のダイナミクスの追跡

RPA-CRISPR/Cas12A-SPMとディープラーニングに基づくDNAウイルス検出システム

ラムダを選択cII変異検出システム

要約

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エラー修正 DNA ・ RNA シーケンスを用いた珍しいイベント検出

サイト監督スピン標識および五量体リガンド依存性イオンチャネルのEPR分光研究

qKAT: キラー細胞免疫グロブリン様受容体遺伝子の定量的半自動化された入力

米の耕作集団における高解像度溶融による変異の同定

精製造血幹細胞/前駆細胞のDNAの変異を特定する

Identification of Functionally-Relevant Lentivirus Integration Sites in an Insertional Mutagenesis Cell Library

内タンパク質の定方向進化のための変異導入と機能選択プロトコル E.大腸菌

CRISPR-Cas12aと組み合わせたリコンビナーゼポリメラーゼ増幅を用いたフィールド展開可能な カンディダトゥス ・リベリバクター・アジアティカス検出(英語)

実験的に進化した集団における構造変異のダイナミクスの追跡

RPA-CRISPR/Cas12A-SPMとディープラーニングに基づくDNAウイルス検出システム

CRISPR-Cas12aと組み合わせたリコンビナーゼポリメラーゼ増幅を用いたフィールド展開可能なカンディダトゥス・リベリバクター・アジアティカス検出(英語)